The kit includes HS Dye, HS Dilution Buffer, and two DNA Standards. The assay is accurate for DNA concentrations from 10 pg/µL to 100 ng/µL, and is highly selective for double-stranded DNA over RNA.
Features
Optimized condition for using with the Qubit® Fluorometer
Uses the Qubit® dsDNA High Sensitivity assay setting
Linear range: 0.2-100 ng dsDNA
Cuts costs by 60%
DNA selectivity and sensitivity of the HS kit. A series of input DNA (20, 40, 60, 80, 100, and 120 ng) was used with or without the contamination of RNA.
Comparison of dsDNA HS kits. The performance of the BioDynami dsDNA HS kit is nearly identical to that of Thermo Fisher’s Qubit dsDNA HS kit.
Other Products
Propargyl-PEG4-Maleimide
Product Info
Document
Product Info
Propargyl-PEG4-Maleimide has a propargyl group and a maleimide group. The propargyl group reacts with azide compounds or biomolecules under the catalyzation of copper in Click Chemistry reactions. The maleimide group enables the formation of a covalent bond with biomolecules containing a thiol group. Reagent grade, for research purpose. Please contact us for GMP-grade inquiries.
Document
Propargyl-PEG4-Maleimide has a propargyl group and a maleimide group. The propargyl group reacts with azide compounds or biomolecules under the catalyzation of copper in Click Chemistry reactions. The maleimide group enables the formation of a covalent bond with biomolecules containing a thiol group. Reagent grade, for research purpose. Please contact us for GMP-grade inquiries.
This product is suitable for rapid extraction of DNA from plant and fungal samples. The kit is based on silica gel column purification technology. The whole extraction process only takes 60-90 minutes. DNA can be used directly for PCR, quantitative PCR, southern Blot, test of virus DNA and so on.
Details
Specifications
Features
Specifications
Main Functions
Isolation total DNA from 50mg simple plant using 96 well bind plate
Plantmaterial is first mechanically disrupted and then lysed by addition of lysis buffer and incubation. RNase A in the lysis buffer digests the RNA in the sample. After lysis, proteins and polysaccharides are salt-precipitated. Binding buffer and ethanol are added to the cleared lysate to promote binding of the DNA to the HiPure membrane. The sample is then applied to a column and then centrifuged. DNA binds to the membrane, while contaminants such as proteins and polysaccharides are efficiently removed by 2 wash steps. Pure DNA is eluted in a small volume of low-salt buffer or water.
Advantages
High quality DNA – meet a variety of downstream applications, including PCR, qPCR, enzyme digestion, blot hybridization, etc.
High throughput – 96 samples can be processed simultaneously by 96 well plate
High permeability – using composite membrane technology, no hole plugging
Kit Contents
Contents
D316702
D316703
Purification Times
4 x 96 Preps
20 x 96 Preps
RNase Solution
5 ml
22 ml
Elution Buffer
120 ml
500 ml
Buffer SPL
200 ml
2 x 500 ml
Buffer PS
100 ml
400 ml
Buffer GW1
220 ml
5 x 220 ml
Buffer GW2
100 ml
3 x 100 ml
1.6ml Collection Plate
4
20
HiPure gDNA Plate
4
20
2.2ml Collection Plate(DW Plate)
8
40
0.8ml Collection Plate(DW Plate)
4
20
Sealing Film
20
100
Storage and Stability
RNase solution should be stored at 2-8°C upon arrival. However, short-term storage (up to 24 weeks) at room temperature (15-25°C) does not affect its performance. The remaining kit components can be stored dry at room temperature (15-25°C) and are stable for at least 18 months under these conditions.
This product is suitable for rapid extraction of DNA from plant and fungal samples. The kit is based on silica gel column purification technology. The whole extraction process only takes 60-90 minutes. DNA can be used directly for PCR, quantitative PCR, southern Blot, test of virus DNA and so on.
