The dsDNA Quantification Broad Range Kit and dsDNA Quantification High Sensitivity Kit are developed for double stranded DNA quantification using microplate readers. The DNA Quantification BR kit includes BR Dye, BR Dilution Buffer, and BR DNA Standards. The DNA Quantification HS kit includes HS Dye, HS Dilution Buffer, and HS DNA Standards. Simply dilute the Dye with the Dilution Buffer, add DNA sample, then read the concentration using microplate reader. The BR assay is accurate in the linear range from 4 to 1000 ng and the HS assay is accurate in the linear range from 0.2 to 100 ng. Both kits are highly selective for double-stranded DNA over RNA.
The Quantification Kits have several advantages over traditional DNA quantitation such as sensitivity, stability, and tolerance of contaminants.
Both kits reduce the cost of DNA quantification by 60% as compared to other brands.
Selectivity and sensitivity of the kits
Save 60% of the costs.
The performance of the kit is nearly identical to that of Thermo Fisher’s kit (figure below).
Comparison of BioDynami dsDNA Quantification Broad Range Kit with Thermo Fisher kit.
Comparison of BioDynami dsDNA Quantification High Sensitivity Kit with Thermo Fisher kit.
Common contaminants such as salts, free nucleotides, solvents, detergents, RNA, single stranded DNA, or protein are well tolerated in the assay (Table 1).
Other Products
endo-BCN-PEG4-t-butyl ester
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Product Info
endo-BCN-PEG4-t-butyl ester is a click chemistry linker containing a BCN group and a t-butyl protected carboxyl group. The BCN group can react with azide-tagged molecules. The protected carboxyl group (COOH) prevents self coupling or polymerization under standard acid/amine or acid/hydroxyl coupling conditions. The t-butyl ester can be converted to free acid under acidc condition. Reagent grade, for research purpose. Please contact us for GMP-grade inquiries.
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endo-BCN-PEG4-t-butyl ester is a click chemistry linker containing a BCN group and a t-butyl protected carboxyl group. The BCN group can react with azide-tagged molecules. The protected carboxyl group (COOH) prevents self coupling or polymerization under standard acid/amine or acid/hydroxyl coupling conditions. The t-butyl ester can be converted to free acid under acidc condition. Reagent grade, for research purpose. Please contact us for GMP-grade inquiries.
NGS Cell Free DNA Library Prep Kit (illumina and MGI Platforms)
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The NGS Cell Free DNA Library Prep Kit (illumina and MGI Platforms) was developed for the construction of high quality cell-free DNA (cfDNA) libraries using 1 ng to 50 ng of cell-free DNA as input. The kit has a simple work flow and a fast procedure. Multiplexing of the cell free DNA library is possible based on the index type.
NGS Cell Free DNA Library Prep Kit Workflow
The main source of cell-free DNA (cfDNA) is derived from apoptotic hematopoietic cells in blood and found in the plasma. The length of the cfDNA is about 150-200 bp in length. Circulating tumor DNA (ctDNA) derived from malignant tumors is a part of cfDNA. Both cfDNA and ctDNA can be used as a noninvasive biomarker since it offers a better approach in comparison to invasive tissue biopsies.
NGS has been used for cfDNA and ctDNA sequencing in the field of liquid biopsy as it provides a whole genome level of molecular profiling. One of the hurdles for cfDNA sequencing is the difficulty of library preparation from the limited amount of cfDNA obtained from plasma. Our cell-free NGS kit makes it easy to get enough libraries from limited input in just 1.5 hours.
Three index types are available for the NGS Cell Free DNA Library Prep Kit of the illumina platform:
Non-index (Cat.# 30029): Libraries do not have index.
Index(Cat.# 30031): Each of our index primers contains a unique 6-base index sequence that can be used for sample identification. Total 48 library multiplexing is possible. Index information can be downloaded here.
Unique dual index (Cat.# 30033): Cell-free DNA library multiplexing up to 96 samples is possible with the unique dual indexes. We have developed a Four-Base Difference Index System. The system have at least 4 bases different from each other in the 8 bases index length. The primers effectively minimize sequencing errors such as mis-assignment, index hopping, index contamination etc. Index information can be downloaded here.
Indexes are available for the MGI platform kits (Cat.# 34031).
Kit advantages:
Fast and simple protocol
Hands-on time is around 10 minutes
The total protocol time is only 1.5 hours
Easy procedure based on:
Ready-to-use master mix for easy setup of reactions
Less reaction components to simplify bench work
Less magnetic beads used for cleanup procedures: This can reduce more than 50% of the beads consumption
Guaranteed high library conversion efficiency
Low input cell-free DNA: From 1 ng to 50 ng
Comparison of library conversion efficiency with different samples under the same condition.
Comparison of library yield with different samples under the same condition.
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The NGS Cell Free DNA Library Prep Kit (illumina and MGI Platforms) was developed for the construction of high quality cell-free DNA (cfDNA) libraries using 1 ng to 50 ng of cell-free DNA as input. The kit has a simple work flow and a fast procedure. Multiplexing of the cell free DNA library is possible based on the index type.
Attogene has developed the first of its kind and patent pending technology that uses a novel RNA/DNA substrate tagged on the 5′ and 3′ end with a Biotin that is attached to our streptavidin colloidal gold reporter molecules and a test line tag. In the absence of RNases H, the gold tagged RNA/DNA molecule will flow up the lateral flow strip and bind to the test line using an anti tag antibody (INDICATING that NO RNase H is detected). When RNases H is present or added, however, the RNA strand of the RNA/DNA substrate is cleaved, and the Biotin tagged 5′ and 3′ tags are spatially separated in solution cauing the test line to disapear.
This test can be used to rapidly and efficiently detect ribonucleases H activity and is a perfect tool for monitoring unit activity or residual RNAse H activity.
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RNase H activity in a convenient and sensitive colormetric assay that delivers results in real time. Great for Quality Testing for RNase H activity in enzyme preparations and determination of RNase H unit activity.
