The dsDNA Quantification Broad Range Kit and dsDNA Quantification High Sensitivity Kit are developed for double stranded DNA quantification using microplate readers. The DNA Quantification BR kit includes BR Dye, BR Dilution Buffer, and BR DNA Standards. The DNA Quantification HS kit includes HS Dye, HS Dilution Buffer, and HS DNA Standards. Simply dilute the Dye with the Dilution Buffer, add DNA sample, then read the concentration using microplate reader. The BR assay is accurate in the linear range from 4 to 1000 ng and the HS assay is accurate in the linear range from 0.2 to 100 ng. Both kits are highly selective for double-stranded DNA over RNA.
The Quantification Kits have several advantages over traditional DNA quantitation such as sensitivity, stability, and tolerance of contaminants.
Both kits reduce the cost of DNA quantification by 60% as compared to other brands.
Selectivity and sensitivity of the kits
Save 60% of the costs.
The performance of the kit is nearly identical to that of Thermo Fisher’s kit (figure below).
Comparison of BioDynami dsDNA Quantification Broad Range Kit with Thermo Fisher kit.
Comparison of BioDynami dsDNA Quantification High Sensitivity Kit with Thermo Fisher kit.
Common contaminants such as salts, free nucleotides, solvents, detergents, RNA, single stranded DNA, or protein are well tolerated in the assay (Table 1).
Other Products
Total Dietary Fiber Assay Kit
Product Info
Document
Product Info
K-TDFR-200A
SKU: 700004347
Content:
100 assays / 200 assays
Shipping Temperature:
Ambient
Storage Temperature:
Short term stability: 2-8oC, Long term stability: See individual component labels
Stability:
> 2 years under recommended storage conditions
Analyte:
Dietary Fiber
Assay Format:
Enzymatic
Detection Method:
Gravimetric
Signal Response:
Increase
Limit of Detection:
0.5 g/100 g
Total Assay Time:
~ 100 min
Application examples:
Food ingredients, food products and other materials.
Method recognition:
AACC Method 32-05.01, AACC Method 32-06.01, AACC Method 32-07.01, AACC Method 32-21.01, AOAC Method 985.29, AOAC Method 991.42, AOAC Method 991.43, AOAC Method 993.19, CODEX Method Type I and GB Standard 5009.88-2014
The Total Dietary Fiber Assay Kit for the analysis of Total, Soluble and Insoluble Dietary Fiberaccording to AOAC and AACC approved methods.
Dietary fiber can generally be described as the carbohydrate content of food that is not digested in the human small intestine. It passes into the large intestine where it is partially or fully fermented. These characteristics of dietary fiber are associated with its numerous well documented health benefits.
Dietary Fiber is a mixture of complex organic substances, including hydrophilic compounds, such as soluble and insoluble polysaccharides and non-digestable oligosaccharides, as well as a range of non-swellable, more or less hydrophobic, compounds such as cutins, suberins and lignins. The procedures for the determination and analysis of total dietary fiber as outlined in our assay protocol are based on the methods of Lee et al.1 and Prosky et al.2,3 (AOAC 991.43, AOAC 985.29, AACC 32-07.01 and AACC 32-05.01). However, the enzymes in the Megazyme Total Dietary Fiber Kit can also be used in other dietary fiber analytical methods such as AACC Method 32-21.01 and AACC Method 32-06.01.
1. Association of Official Analytical Chemists. (1985). Official Methods of Analysis, 14th ed., 1st suppl. Secs. 43, A14-43, A20, p.399. 2. Association of Official Analytical Chemists. (1986). Changes in methods. J. Assoc. Off. Anal. Chem., 69, 370. 3. Association of Official Analytical Chemists. (1987). Changes in methods. J. Assoc. Off. Anal. Chem., 70, 393.
See General Referee Reports: Journal of AOAC INTERNATIONAL, Vol. 81, No. 1, 1998.
Two separate methods are described in the associated assay protocol:
METHOD 1: DETERMINATION OF TOTAL, SOLUBLE AND INSOLUBLE DIETARY FIBER Based on AOAC Method 991.43 “Total, Soluble, and Insoluble Dietary Fiber in Foods” (First Action 1991) and AACC Method 32-07.01 “Determination of Soluble, Insoluble, and Total Dietary Fiber in Foods and Food Products” (Final Approval 10-16-91).
METHOD 2: DETERMINATION OF TOTAL DIETARY FIBER Based on AACC method 32-05.01 and AOAC Method 985.29.
Note that a letter of endorsement from the original method developer, Dr. Leon Prosky, is included in the Documents Tab.
This ELISA kit is intended for the quantitative detection of IgG antibodies against parasites of Ascaris genus in human serum.
This product is manufactured by Bordier Affinity Products in Switzerland and distributed in Germany exclusively by Milenia Biotec.
Method/Platform
ELISA in microplate format
Range/Assay Sensivity
pNPP, λ=405 nm
Test Principle
Specific antibodies in the sample bind to Ascaris solubles coelomics antigens. The presence of parasite specific antibodies is detected with a Protein A alkaline phosphatase conjugate.
12 x 8 strips (96 tests) This ELISA kit is intended for the quantitative detection of IgG antibodies against parasites of Ascaris genus in human serum.
This product is manufactured by Bordier Affinity Products in Switzerland and distributed in Germany exclusively by Milenia Biotec.
50 assays of each (manual) / 500 assays of each (microplate)
Content:
50 assays of each (manual) / 500 assays of each (microplate)
Shipping Temperature:
Ambient
Storage Temperature:
Short term stability: 2-8oC, Long term stability: See individual component labels
Stability:
> 2 years under recommended storage conditions
Analyte:
Ammonia, L-Glutamine
Assay Format:
Spectrophotometer, Microplate
Detection Method:
Absorbance
Wavelength (nm):
340
Signal Response:
Decrease
Linear Range:
1 to 40 µg of L-glutamine per assay
Limit of Detection:
0.54 mg/L (L-glutamine), 0.06 mg/L (ammonia)
Reaction Time (min):
~ 10 min
Application examples:
Cell culture media and cultures, dietary supplements, vegetables and other materials (e.g. biological samples, etc.).
Method recognition:
Novel method
This product has been discontinued.
The L-Glutamine/Ammonia (Rapid) test kit is a novel method for the specific, convenient, cost effective and rapid measurement and analysis of L-glutamine and ammonia in culture media/supernatants and other materials.
Note for Content: The number of manual tests per kit can be doubled if all volumes are halved. This can be readily accommodated using the MegaQuantTM Wave Spectrophotometer (D-MQWAVE).
Extended cofactors stability. Dissolved cofactors stable for > 1 year at 4oC.
Very rapid reaction due to use of high activity glutaminase and uninhibited glutamate dehydrogense
All enzymes supplied as stabilised suspensions
Only enzymatic kit available
Very cost effective
All reagents stable for > 2 years after preparation
Mega-Calc™ software tool is available from our website for hassle-free raw data processing
Standard included
Document
The L-Glutamine/Ammonia (Rapid) test kit is a novel method for the specific, convenient, cost effective and rapid measurement and analysis of L-glutamine and ammonia in culture media/supernatants and other materials.
