Easy Operate Stable Isothermal Amplification Kit No Expensive PCR Apparatus
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Detail
Kit Storage and Term of Validity
Storage term: stored at ≤-20℃,keep away from light, avoid heavy weight and repeated freezing and thawing.
Term of Validity: 14 months
Isothermal Nucleic Acid Principle Summary
The kit is based on rapid nucleic acid amplification technology at room temperature and constant temperature, its principle is that at room and constant temperature, the recombinase and primer form a protein/single-stranded nucleotide complex Rec/ssDNA, and invade the double-stranded DNA template with the help of auxiliary proteins and single-stranded binding protein SSB; then form a D-loop region at the invasion point and start to scan the DNA duplex, after finding the target region complementary to the primer and disintegration of the complex Rec/ssDNA, the polymerase also binds to the 3′ end of the primer to start the chain extension.
Isothermal Nucleic Acid Product Features
1/ High sensitivity and specificity, short reaction time.
2/ The reagent form is freeze-dried, stable and easy to operate.
3/ The reaction can be operated by metal bath and water bath pot without purchasing expensive PCR apparatus.
Technical Parameters:
Parameters
Details
Product Name
DNA Isothermal Amplification Kit Basic
Manufacturer
Amp-future
Storage Temperature
-20°C
Kit Components
Enzymes, Buffers ,Reagents
Packaging
48 Tests/box
Detection Limit
500-1000copies/µL
Shipping
ICE
Test Time
5-20mins
Isothermal Nucleic Acid Applications
Suitable for DNA isothermal rapid amplification kit(Basic type)
Primer: Require pair of nucleotide primers with the length of 25-35 bp.
Colloidal gold probe:Require a sequence of 46-52nt in length
DNA basic kit reaction temperature is 39 to 42℃ and time is 5-20 minutes.
Notes
1/ Please avoid nucleic acid contamination and set blank control during reaction due to the high sensitivity of the kit.
2/ Please take out the required quantity of MIRA reaction units for the experiment, and put the rest under storage conditions when performing the experiment.
Other Products
APOPercentage apoptosis assay kit
Product Info
Document
Product Info
What is Apoptosis?
Apoptosis is an essentially normal physiological process that removes now redundant, cells, particularly during embryonic development and early growth. In adult animals the process removes cells that are irreparable. The apoptotic process is also involved in many major diseases such as cancer, where transformed tumour cells have their apoptotic process disabled, permitting cell cycling to continue unchecked. In contrast some forms of senile dementia may result from excessive apoptotic induction of neural cells.
The apoptotic process in mammalian cells is a rapid event (2‐4 hours). Within this short time span an apparently viable cell can be quietly dismantled, to disappear leaving no visible trace of its former existence.
How is apoptosis detected or measured?
An apoptosis cascade of activators, effectors and regulators has been identified. This in turn led to a range of apoptosis assays being devised to detect and monitor these events. Some laboratories will employ two distinct assays, one selected to detect early (initiation) apoptotic events, while a second assay will target a later (execution) event. Apoptosis assays, based on methodology, can be classified into four major inter‐linked groups:
[1] DNA fragmentation (electrophoresis and nick end labelling, TUNEL).
[2] Apoptotic proteases (fluorescently labelled antibodies to the caspases).
[3] Flow cytometric analysis (FACS, incorporating other group assays).
Biocolor’s APOPercentage assay is based on the latter. Further information can be found under the ‘Mode of Action’ Tab.
How does APOPercentage detect apoptosis?
The mammalian cell membrane has been described as a semi‐fluid mosaic structure, composed of phospholipids with a diverse group of inserted proteins and some cholesterol. The phospholipids are the major components of the membrane and are arranged in the form of a ‘bi‐layer’; which is asymmetric in composition, structure, and function.
To ensure normal transmembrane functions the phospholipids must be maintained in an asymmetric composition. The process is regulated by ‘flippases’, which catalyse the active transport of aminophospholipids from the outer to inner monolayer. However, in cells undergoing apoptosis, flippase is overwhelmed by the action of another enzyme, termed ‘floppase’ or ‘scramblase’. The net effect is a scrambling of the phospholipid distribution between the inner and outer monolayers.
Cell membrane changes during apoptosis
The APOPercentage assay utilises an intense, pink-coloured dye reagent which is taken up during in-vitro culture by apoptosis-committed cells. This uptake occurs at the stage of Phosphatidylserine transmembrane movement, as produced by the flipflop mechanism. Dye uptake continues until blebbing occurs. No further dye can then enter the now defunct cell and the dye that has accumulated within the cell is not released (unlike necrotic cells which release dye).
Since the dye reagent is excluded or not retained by healthy or necrotic cells it therefore acts as a specific label for apoptotic cells.
How are APOPercentage-labelled cells quantified?
Labelled apoptosis cells may then by conveniently analysed by the following methods:
Direct Analysis The intense pink colour of the labelled cells can be visually assessed using brightfield microscopy. Apoptosis in substrate-adherent cell populations is therefore readily quantified using image analysis techniques. This technique is the most sensitive with the ability of detecting one single apoptotic cell per well.
Colorimetry protocol Dye that accumulates within apoptotic cells is released into solution via addition of Dye Release Reagent. The concentration of this intracellular dye is then measured at 550nm using a microplate colorimeter/spectrophotometer.
NB: The APOPercentage assay kit does NOT require the use of a Flow Cytometer.
Limit of Detection
A single cell (via image analysis method)
Detection Method
Colorimetric (550nm) (Endpoint) or Image Analysis based
Measurements per kit
Sufficient for 4×24 well plates or 6×96 well plates
Suitable Samples
Adherent mammalian cells (in-vitro)
APOPercentage kit contents:
1. APOPercentage Dye (1x5ml)
2. Dye Release Reagent (1x150ml)
3. Phosphate Buffered Saline (PBS) (1x120ml)
4. 24-well starter plate.
5. Assay kit manual.
The Colorimetric Protocol requires a Microplate Colorimeter / Spectrophotometer.
Additional 96-well plates will be required for use when reading dye absorbance values.
The Direct Detection Protocol Requires an inverted stage microscope with an attached digital camera.
NB: Additional reagents (typically culture medium and suitable apoptosis treatments) may be required for sample preparation prior to assay. Consult manual or contact us for further details.
Document
The APOPercentage™ Apoptosis kit is a dye-based, colorimetric assay for detection and measurement of apoptosis (programmed cell death) during in-vitro cell culture.
Yingtai pilot-type freeze dryer completely simulates the technical requirements of the industrial freeze dryer,with a compact and reasonable design,a large redundancy range,and conforms to GMP and FDA standards.The ice condensation capacity is 4~18kg,and the WRL controller and liquid temperature control shelf are adopted,so that the process of pre-freezing and freeze-drying can be precisely controlled on the main machine,and the process results are quite comparable with the production machine.In addition to supporting the eutectic point test system,pressure rise and pressure comparison method to determine the freeze-drying end point and freeze-drying curve recording software,hallan pilot-type freeze dryer can also be equipped with engineering WRL control software to directly Graphical computer control the freeze-drying process.It can be configured with H202 sterilization and high-pressure steam sterilization orintegrated glove box system, which can be used for clean room operation through the wall.The cold trap and freeze-drying chamber are all designed with 316L stainless steel to ensure the cleanliness of the chamber and good ultimate vacuum and vacuum leakage rate to the greatest extent.
Refrigeration Series
● Dual compressors,Secop special compressors,air cooling (water cooling system is optional)
Silicone oil conducts heat,with uniform temperature and accuracy up to 0.1°C.Silicone oil has a wide temperature range,up to-100°C~+600°C.
● Shelf refrigeration system and cold trap refrigeration system are completely independent,without any switching,refrigeration temperature is lower and more stable.
Vacuum series
● Vacuum pump:German Pfeiffer anti-corrosion,anti-oil return function vacuum pump.(Vacuum pump can be selected according to user needs)
● Pumping rate:9m³/h~62m³/h,multiple specifications are optional,the ultimate vacuum can reach 2×10-3mbar,and the oil mist filter is standard.
● The vacuum pipelines are all high-vacuum stainless steel vacuum tubes, which can effectively prevent vacuum leakage and corrosion.
● The double anti-oil return device completely eliminates the damage and
equipment and samples.
● Automatic backfill air device,the system has a built-in High efficiency particulate air(HEPA)filter to ensure the complete cleanliness of backfill air.
● The vacuum stabilization system can stabilize the vacuum degree of the freeze-drying process through the micro-pass vacuum control valve to ensure that the samples will not be affected by excessive fluctuations in the vacuum degree.
● Condensed liquid collection and discharge system,automatically completes the collection and discharge of condensed liquid after freeze-drying.
Control SystemCharacteristic
● Control:Automatic/Manual
● Can store at least 100 independent freeze-drying programs,each program contains 32 program segments (Can be
customized according to user needs),each program segment corresponds to a processing stage (pre-freezing,main
drying,post-drying).and Include the following segment values:Cycles,shelf temperature,vacuum and safety pressure
● All the equipment can be recorded in real time and the corresponding freeze-drying curves and data tables can be generated after the end.The records of all data and curves comply with the corresponding regulations and standards.
● It has hierarchical management authority,hierarchical management allocation authority and usage process tracking authority
● Remote System
O Fully support 2G/4G/GSM/Wi-Fivarious connection modes
o Users can remotely view all data on the device and operate it
o Technicians can remotely perform maintenance and fault detection on equipment,and can remotely guide and train users
o Can remotely upgrade and change the system of the equipment
(Optional)Sufficient amount and time for the program to run itself
User function customization
Can be customized and modified according to user needs
Sterilization method
HzOz/steam sterilization
Note:”/”optional.Pilot models can be customized according to user needs
Document
Yingtai pilot-type freeze dryer completely simulates the technical requirements of the industrial freeze dryer,with a compact and reasonable design,a large redundancy range,and conforms to GMP and FDA standards.The ice condensation capacity is 4~18kg,and the WRL controller and liquid temperature control shelf are adopted,so that the process of pre-freezing and freeze-drying can be precisely controlled on the main machine,and the process results are quite comparable with the production machine.In addition to supporting the eutectic point test system,pressure rise and pressure comparison method to determine the freeze-drying end point and freeze-drying curve recording software,hallan pilot-type freeze dryer can also be equipped with engineering WRL control software to directly Graphical computer control the freeze-drying process.It can be configured with H202 sterilization and high-pressure steam sterilization orintegrated glove box system, which can be used for clean room operation through the wall.The cold trap and freeze-drying chamber are all designed with 316L stainless steel to ensure the cleanliness of the chamber and good ultimate vacuum and vacuum leakage rate to the greatest extent.
● The double anti-oil return device completely eliminates the damage and
