endo-BCN-PEG12-acid is amonodisperse PEG reagent containing a BCN group and a terminal carboxylic acid. The terminal carboxylic acid can react with primary amine groups in the presence of activators (e.g. EDC, or HATU) to form a stable amide bond. The BCN group enable click chemistry with azide -tagged molecules. The hydrophilic PEG spacer increases solubility in aqueous media. Reagent grade, for research purpose. Please contact us for GMP-grade inquiries.
endo-BCN-PEG12-acid is amonodisperse PEG reagent containing a BCN group and a terminal carboxylic acid. The terminal carboxylic acid can react with primary amine groups in the presence of activators (e.g. EDC, or HATU) to form a stable amide bond. The BCN group enable click chemistry with azide -tagged molecules. The hydrophilic PEG spacer increases solubility in aqueous media. Reagent grade, for research purpose. Please contact us for GMP-grade inquiries.
K-GLNAM
SKU: 700004295
50 assays of each (manual) / 500 assays of each (microplate)
| Content: | 50 assays of each (manual) / 500 assays of each (microplate) |
| Shipping Temperature: | Ambient |
| Storage Temperature: | Short term stability: 2-8oC, Long term stability: See individual component labels |
| Stability: | > 2 years under recommended storage conditions |
| Analyte: | Ammonia, L-Glutamine |
| Assay Format: | Spectrophotometer, Microplate |
| Detection Method: | Absorbance |
| Wavelength (nm): | 340 |
| Signal Response: | Decrease |
| Linear Range: | 1 to 40 µg of L-glutamine per assay |
| Limit of Detection: | 0.54 mg/L (L-glutamine), 0.06 mg/L (ammonia) |
| Reaction Time (min): | ~ 10 min |
| Application examples: | Cell culture media and cultures, dietary supplements, vegetables and other materials (e.g. biological samples, etc.). |
| Method recognition: | Novel method |
This product has been discontinued.
The L-Glutamine/Ammonia (Rapid) test kit is a novel method for the specific, convenient, cost effective and rapid measurement and analysis of L-glutamine and ammonia in culture media/supernatants and other materials.
Note for Content: The number of manual tests per kit can be doubled if all volumes are halved. This can be readily accommodated using the MegaQuantTM Wave Spectrophotometer (D-MQWAVE).
See our full list of assay kits.
Advantages
The L-Glutamine/Ammonia (Rapid) test kit is a novel method for the specific, convenient, cost effective and rapid measurement and analysis of L-glutamine and ammonia in culture media/supernatants and other materials.
Protease K is a serine protease with high enzymatic activity and wide substrate specificity. It can preferentially decompose ester and peptide bonds adjacent to the ends of hydrophobic amino acids, sulfur-containing amino acids and aromatic amino acids C, and is often used to degrade proteins to produce short peptides. It has the typical catalytic triad Asp39-His69-Ser224 characteristic of serine proteases and two Ca2+ binding sites around the active center increase its stability, allowing it to maintain high enzyme activity under a wider range of conditions.
Appearance: Colorless to light brown liquid; Specific activity: ≥800 U/mL; Protein concentration: ≥20 mg/mL
Source: Tritirachiumalbum; Category: EC 3.4.21.64; Molecular weight: 29 kDa(SDS-PAGE); Isoelectric point: 7.81; The optimal pH: 7.0-12.0 has high activity; Optimum temperature: 65℃; pH stability: pH 4.5-12.5(25℃,16 h); Thermal stability: stable below 50℃ (pH 8.0,30 min); Storage stability: 25℃ a year activity of more than 90% activator SDS, urea; Inhibitors: diisopropyl fluorophosphate (DFIP), benzoyl fluoride (PMSF); Storage conditions: 2-8℃, valid for 24 months.
Wear protective gloves and goggles when using, and keep well ventilated after use. This product may cause allergic skin reactions.
Note: Price not include shipment & duty, contact us to get full quote.
83, On-nut 88/2 Prawet Sub-district, Prawet District, Bangkok, 10250, Thailand
Tel : 081-875-1869 , 02-328-7179
Email : hej@a3p-scientific.com
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