Overview

Optically clear heat sealing film which is non-peelable and non-pierceable. The seal is suitable for qPCR, optical applications and storage.

• Heat sealing offers a 100% effective method for plate sealing for a complete seal integrity, as well as being quick and cost effective
• Our ClearASeal Weld is an optically clear polymer film forming a permanent seal to polypropylene plates
• The optical clarity of this seal enables its use for sealing plates required for imaging use, including fluorescent detection methods such as qPCR and colorimetric assays
• The ClearASeal Weld forms a complete seal to a plate, enabling both low and very high temperature uses, including low temperature storage and high temperature incubations
• This seal is suitable for PCR/qPCR, even without the use of a pressurized heated lid, and is 100% effective when used in water bath thermal cyclers
• The permanent nature of this 100% effective seal renders it suitable for the storage and disposal of hazardous material
• ClearASeal Weld demonstrates a good solvent resistance and can be utilized for long term compound storage
• This seal is available as sheets, for use with manual and semi-automated sealers, such as our HeatASeal 500 Sealing Machine
• Also available in multiple roll formats compatible with specified automated heat sealers, such as our Wasp or Chameleon XT

Optically clear heat sealing film which is non-peelable and non-pierceable. The seal is suitable for qPCR, optical applications and storage.

Introduction

MagZol Reagent is a reagent system for the isolation of total RNA from cells and tissues. The reagent, a single-phase solution consisting of phenol and guanidine isothiocyanate, is modification of the single-step RNA isolation method developed by Chomczynski and Sacchi. The sample is homogenized and lysed in MagZol Reagent which maintains the integrity of the RNA, while disrupting and denaturing endogenous RNases and other cellular components. Extraction of the lysate with chloroform further denatures proteins and separates the mixture into an organic and an aqueous phase. RNA remains exclusively in the aqueous phase, and is subsequently recovered by isopropanol.

This method is suitable for small quantities of tissue (<100mg) and cells (<5 X10⁶), and large quantities of tissue (up to 1g) and cells (<10⁸), of human, animal, plant, or bacterial origin. The simplicity of the MagZol Reagent method allows simultaneous processing of a large number of samples. The entire procedure can be completed in one hour. Total RNA prepared in this manner can be used for Northern blot analysis, dot blot hybridization, poly(A) + selection, in vitro translation, RNase protection assay, and molecular cloning. For use in amplification by thermal cycling, treatment of the isolated RNA with RNase-free DNase I is recommended when the two amplimers lie within a single exon.

Details

Specifications

Advantages

• Flexible – sample amount can be adjusted according to the demand
• Cost performance -the most economical nucleic acid extraction technology

Storage and Stability

MagZol Reagent should be stored at 2-8°C upon arrival and is stable for at least 24 months under the condition. However, short-term storage (up to 12 weeks) at room temperature (15-25°C) does not affect its performance.

MagZol Reagent is a reagent system for the isolation of total RNA from cells and tissues. The reagent, a single-phase solution consisting of phenol and guanidine isothiocyanate, is modification of the single-step RNA isolation method developed by Chomczynski and Sacchi. The sample is homogenized and lysed in MagZol Reagent which maintains the integrity of the RNA, while disrupting and denaturing endogenous RNases and other cellular components. Extraction of the lysate with chloroform further denatures proteins and separates the mixture into an organic and an aqueous phase. RNA remains exclusively in the aqueous phase, and is subsequently recovered by isopropanol.

Description

Attogene’s Mercury Lateral Flow Kit can be used for the screening of Mercury in water samples at 10 ppb in the field.

Mercury contamination is a serious worldwide environmental problem. As it is difficult to detoxify by chemical or biological methods, gradual Mercury ion accumulation in the nervous and cardiovascular systems of the human body can subsequently cause serious diseases.   Long-term health consequences of drinking Mercury-contaminated food and water include brain, heart, kidney, lungs  and immune system problems for adults, and the physical and mental development delays in infants and children.  Attogene’s Mercury Lateral Flow test gives results conforming of 10ppb or greater.  Using the supplied pipette, simply fill the vial with your water sample, place the water into the sample port and wait 5 minutes.

Applications for Fish and Shellfish possible:

Mercury Levels in Commercial Fish and Shellfish (1990-2012)

Samples requiring regulatory action should be confirmed.

Screening of Mercury in water samples at 10 ppb
Format: 10 tests (5 tests/5 controls)
Run Time: 5 Minutes