endo-BCN-PEG2-alcohol

Biotin-PEG8-alkyne is a PEG linker containing a biotin group and a terminal alkyne. Biotin is useful for affinity-based applications such as pull-down assays while terminal alkynes are used in copper (I) click chemistry with azide groups on a target molecule. The inclusion of a PEG linker in this molecule improves its solubility in water.

Biotin-PEG8-alkyne is a PEG linker containing a biotin group and a terminal alkyne. Biotin is useful for affinity-based applications such as pull-down assays while terminal alkynes are used in copper (I) click chemistry with azide groups on a target molecule. The inclusion of a PEG linker in this molecule improves its solubility in water.

Overview

• Ready-to-use
• Quantitative
• Highly Stable
• Precise
• 10 discrete bands from 50 bp to 500 bp
• Higher intensity reference band at 250 bp

The 50 bp DNA Ladder is prepared to ensure quality and batch-to-batch consistency. This Ladder contains ten discrete fragments ranging from 50 bp to 500 bp with a higher intensity reference band at 250 bp. This Ladder is ideal for quick sizing of PCR products

Contents
1mL of premixed DNA ladder (0.5µg/10µL) in loading buffer (10mM EDTA, 10% glycerol, 0.015% bromophenol blue, and 0.17% SDS).

Details

Supporting Data

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50 bp DNA Ladder (Cat# 13525) – 100 loads

Ladder Properties:
• Ten discrete bands, ranging from 50 bp to 500 bp
• Higher intensity band at 250 bp for easy reference

Fragment	Size (bp)	Mass (ng)
1	500	79
2	450	67
3	400	59
4	350	55
5	300	49
6	250	76
7	200	33
8	150	22
9	100	31
10	50	29

Recommended Use:

Mix thoroughly. For best results, load 10µL of DNA ladder per well. For precise mass determination with a densitometer, stain gel after electrophoresis using 0.5µg/mL ethidium bromide for 30-40 minutes. The table above shows the size and mass for each band based on 10µL ladder per well.

Storage: 

Stable at room temperature. For longer term storage, -20°C is recommended.

This ladder was standardized using 10µL of DNA per lane on a 0.8 cm thick, 13 x 15 cm, 1.0% agarose gel run in TAE buffer.

Permagen’s 32-tube PCR Separation rack features all of the same aspects of our MSR812 rack above for easy protocol transition for labs wishing to scale up to four PCR strips at a time, or up to 32 individual PCR tubes.

Features include solid aluminum alloy design with hard coat finish for years of trouble-free use, rubber feet to help prevent slipping on work bench, less tippy than common plastic products, and fast separations using any magnetic beads

SKU #

MSR402

Features

• Aluminum alloy design for years of trouble-free use
• Fast magnetic bead separations
• Held to tighter tolerances for more consistent results
• Pulls beads to side of wells for easy tip tracking down front side
• Rubber feet to help prevent slippage
• Stable design
• Angled tube holes which allow the tubes to come close to magnets
• Easy protocol integration from our MSR812 rack

Compatibility

• Up to 32 Individual PCR tubes
• Up to four, 8-strip PCR tube strips
• Any Magnetic Beads

Volumes

Maximum  – .2 mL 

Minimum  – 30 µL 

Permagen’s 32-tube PCR Separation rack features all of the same aspects of our MSR812 rack above for easy protocol transition for labs wishing to scale up to four PCR strips at a time, or up to 32 individual PCR tubes.