endo-BCN-PEG2-alcohol is a click chemistry PEG linker. The BCN group is very reactive with azide-tagged molecules. The hydroxyl group enables further derivatization or replacement with other reactive functional groups. Reagent grade, for research purpose. Please contact us for GMP-grade inquiries.
Detail
endo-BCN-PEG2-alcohol is a click chemistry PEG linker. The BCN group is very reactive with azide-tagged molecules. The hydroxyl group enables further derivatization or replacement with other reactive functional groups. Reagent grade, for research purpose. Please contact us for GMP-grade inquiries.
NEST 1.5 mL Microcentrifuge Tube, polypropylene, sterile, DNase, RNase, pyrogen and endotoxin free. Relative centrifugal force (RCF): 30000 x g. Easy-open, secure safelock cap seal. 500 count.
Labware definition is available for immediate use in Opentron’s Labware Library.
Document
NEST 1.5 mL Microcentrifuge Tube, polypropylene, sterile, DNase, RNase, pyrogen and endotoxin free. Relative centrifugal force (RCF): 30000 x g. Easy-open, secure safelock cap seal. 500 count.
Labware definition is available for immediate use in Opentron’s Labware Library.
Isolate high quality DNA from a broad variety of phage strains
High yields of total DNA
Fast and easy processing using a rapid spin-column format
No phenol or chloroform extractions or cesium chloride banding required
High yields of DNA recovered3-15 µg DNA from 106-1010 pfu/ mL of enriched phages
This kit provides a rapid spin column method for the purification of total DNA from a broad spectrum of bacteriophages propagated in bacteria grown in liquid cultures. The DNA is isolated without the use of phenol, chloroform or cesium chloride banding procedures. The spin-column based procedure is rapid and can be completed in less than 45 minutes. The kit is highly efficient for processing small volumes of phage supernatant (500 µL – 1 mL) and with the optional DNase and Proteinase K treatments phage DNA yields are maximized while host DNA contamination is minimized. Purified total phage DNA is of the highest integrity, and can be used in a number of downstream applications including PCR, qPCR, Restriction Fragment Length Polymorphism (RFLP), sequencing, cloning, Southern Blot and more.
3-15 µg DNA from 106-1010 pfu/mL of enriched phages
Time to Complete 10 Purifications
45 minutes
* Average yields will vary depending upon a number conditions used and developmental stage.
Storage Conditions and Product Stability All solutions should be kept tightly sealed and stored at room temperature. This kit is stable for 1 year after the date of shipment.