endo-BCN-PEG3-NHS esterr can be used to label the primary amines (-NH2) of proteins, amine-modified oligonucleotides, and other amine-containing molecules. The BCN group enable copper free click chemistry with azide-tagged biomolecules. The hydrophilic PEG spacer increases solubility in aqueous media. Reagent grade, for research purpose. Please contact us for GMP-grade inquiries.
Detail
endo-BCN-PEG3-NHS esterr can be used to label the primary amines (-NH2) of proteins, amine-modified oligonucleotides, and other amine-containing molecules. The BCN group enable copper free click chemistry with azide-tagged biomolecules. The hydrophilic PEG spacer increases solubility in aqueous media. Reagent grade, for research purpose. Please contact us for GMP-grade inquiries.
Other Products
[RQ2200] ExcelRT™ One-Step RT-qPCR Kit (TaqMan, no ROX), 200 RXN
Product Info
Document
Product Info
Description
The ExcelRT™ One-Step RT-qPCR kit (TaqMan, no ROX) is designed for reverse transcription and quantitative real-time analysis of a specific target RNA by one-step reaction. The ExcelRT™ One-Step RT-qPCR kit (TaqMan, no ROX), consisting of One-Step RT Enzyme Mix and 2X One-Step Master Mix, is a convenient kit designed for highly efficient cDNA synthesis and highly specific real-time PCR in a single tube. The One-Step RT Enzyme Mix contains a thermostable ExcelRT™ Reverse Transcriptase and a RNAok™ RNase inhibitor. Consequently, One-Step RT Enzyme Mix can reverse transcribe RNA to cDNA at a wide temperature range from 42 to 60°C and active against RNase A, RNase B and RNase C. By containing specialized hot-start Taq DNA polymerase, which greatly reduce primer-dimer formation and can be activated within 2 minutes, the 2X One-Step Master Mix features high specificity and is suitable for fast cycle program.
Features
Reverse transcription at wide temperature range (42-60°C)
High specificity
Suitable for fast cycle program
With no ROX reference dye
Storage
Aliquot to avoid multiple freeze-thaw cycles (stable within 30 freeze-thaw cycles)
Protect from light
-20°C for 12 months
Document
The ExcelRT™ One-Step RT-qPCR kit (TaqMan, no ROX) is designed for reverse transcription and quantitative real-time analysis of a specific target RNA by one-step reaction. The ExcelRT™ One-Step RT-qPCR kit (TaqMan, no ROX), consisting of One-Step RT Enzyme Mix and 2X One-Step Master Mix, is a convenient kit designed for highly efficient cDNA synthesis and highly specific real-time PCR in a single tube. The One-Step RT Enzyme Mix contains a thermostable ExcelRT™ Reverse Transcriptase and a RNAok™ RNase inhibitor. Consequently, One-Step RT Enzyme Mix can reverse transcribe RNA to cDNA at a wide temperature range from 42 to 60°C and active against RNase A, RNase B and RNase C. By containing specialized hot-start Taq DNA polymerase, which greatly reduce primer-dimer formation and can be activated within 2 minutes, the 2X One-Step Master Mix features high specificity and is suitable for fast cycle program.
Propargyl-PEG3-CH2CO2H is an PEG linker with an alkyne and carboxylic acid set of functional groups. The alkyne group can participate in copper catalyzed azide-alkyne Click Chemistry to form a stable triazole linkage. The carboxylic acid can react with primary amines to form stable amide bonds; activator (e.g. EDC, or HATU) is needed.
Document
Propargyl-PEG3-CH2CO2H is an PEG linker with an alkyne and carboxylic acid set of functional groups. The alkyne group can participate in copper catalyzed azide-alkyne Click Chemistry to form a stable triazole linkage. The carboxylic acid can react with primary amines to form stable amide bonds; activator (e.g. EDC, or HATU) is needed.
SMOBIO’s B-BOX™ is a long wavelength, blue light LED epi-illuminator. It is compact in design and robust in structure. The B-BOX™ epi-illuminator provides an unprecedented level of safety for its user due to its non-UV light source and a low operating voltage of only 12 Volts, as well as its capability working with non-carcinogenic DNA/ protein dye. The B-BOX™ comes with high quality non-flickering LED light that is highly sensitive for DNA and protein dye. B-BOX™ can greatly ease the routine chore of gel extraction, enabling easy visualization and gel cutting even in bright ambient light. The multi-angle filter plate (Cat. No. VE0102) provides optimal angles for gel cutting, visualization, and documentation. The B-BOX™ also comes with amber colored filter goggles (Cat. No. VE0103) for gel cutting and visualization. The Phox™ Photobox (Cat. No. VE0104) provides a mini darkroom environment for images taken with any smartphone. Finally, a built-in barrier system around the working area helps facilitate cleaning.
Features
Improved cloning efficiency
Compact, lightweight, and portable (less than 1 kg in weight)
Safety features include : 470 nm long wavelength, without any UV radiation hazard to its user
Compatible with non-carcinogenic, non-ethidium bromide DNA staining dye
User friendly: Samples are easy to visualize (when using the filter plate or goggles)
LED light source lasts up to 50,000 hours
Superior detection sensitivity: ≤ 0.04 ng of DNA when using FluoroStain™ DNA Fluorescent Staining Dye, ≤3 ng of protein when using FluoroStain™ Protein Fluorescent Staining Dye (as sensitive as silver stain)
Adjustable and removable filter plate allows for gel cutting, visualization, and documentation
Built-in barrier design, for easy clean up
Visible in bright ambient light
Emphasizes minimal power reliance, low heat generation, with its own built-in heat sink
Document
SMOBIO’s B-BOX™ is a long wavelength, blue light LED epi-illuminator. It is compact in design and robust in structure. The B-BOX™ epi-illuminator provides an unprecedented level of safety for its user due to its non-UV light source and a low operating voltage of only 12 Volts, as well as its capability working with non-carcinogenic DNA/ protein dye. The B-BOX™ comes with high quality non-flickering LED light that is highly sensitive for DNA and protein dye. B-BOX™ can greatly ease the routine chore of gel extraction, enabling easy visualization and gel cutting even in bright ambient light. The multi-angle filter plate (Cat. No. VE0102) provides optimal angles for gel cutting, visualization, and documentation. The B-BOX™ also comes with amber colored filter goggles (Cat. No. VE0103) for gel cutting and visualization. The Phox™ Photobox (Cat. No. VE0104) provides a mini darkroom environment for images taken with any smartphone. Finally, a built-in barrier system around the working area helps facilitate cleaning.