endo-BCN-PEG4-acid

Hydrolysis probe genotyping master mix for use with 5’ nuclease assays such as TaqMan™, BHQ®, BHQplus® & Zen™ probes. Accurate, robust performance at competitive prices.

About

ProbeSure Master Mix combines class-leading performance with the most competitive prices in the market. ProbeSure Master Mix has provided users with accurate, robust, and consistent performance for many years. It has been carefully optimised to work over a wide range of reaction volumes and sample template qualities.

ProbeSure Master Mix is supplied at 2x concentration for convenience and is supplied with the ROX normalising dye at either high level (500 nM final concentration), low level (25 nM final concentration) or without ROX.

Hydrolysis probe genotyping master mix for use with 5’ nuclease assays such as TaqMan™, BHQ®, BHQplus® & Zen™ probes. Accurate, robust performance at competitive prices.

DBCO-NHCO-PEG4-amine is a carboxyl-reactive building block with extended PEG spacer arm. The hydrophilic PEG spacer arm improves water solubility. In the presence of of activators (e.g. EDC, or HATU), this reagent can be used to derivatize carboxyl groups or activated esters (e.g. The NHS ester) through a stable amide bond. DBCO is commonly used for copper-free Click Chemistry reactions. Reagent grade, for research purpose. Please contact us for GMP-grade inquiries.

DBCO-NHCO-PEG4-amine is a carboxyl-reactive building block with extended PEG spacer arm. The hydrophilic PEG spacer arm improves water solubility. In the presence of of activators (e.g. EDC, or HATU), this reagent can be used to derivatize carboxyl groups or activated esters (e.g. The NHS ester) through a stable amide bond. DBCO is commonly used for copper-free Click Chemistry reactions. Reagent grade, for research purpose. Please contact us for GMP-grade inquiries.

Overview

• Protocol optimized for DNA isolated from a diversity of samples including stool, soil, water, saliva, plant, urine, skin, and more
• Simple and quick workflow: library could be prepared in less than 5 hours
• Component of Norgen’s metagenomics workflow
• A single NGS run can be prepared with up to 384 unique dual-index libraries

Sample type purification kit guide 

The 16S V3-V4 Library Preparation Kit for Illumina consists of the reagents and components required for library preparation of the 16S V3-V4 amplicon libraries to be used for next-generation sequencing on Illumina platforms. All molecular reagents including primers, enzyme mixes, indexes, and buffers are provided. Instructions for PCR clean up with the AMPure XP Magnetic Beads (supplied by customer) are also included for rapid purification of nucleic acid products generated at two steps of the workflow. The library prep workflow could be used for purified DNA inputs from different sources including stool, soil, water, saliva, plant, urine, skin swab, vaginal swab, cheek swab, nasal swab, plasma/serum, tongue swab, gum swab, and others.

The 16S V3-V4 Library Preparation Kit for Illumina has a streamlined procedure that reduces the handling time such that the library prep procedure can be completed in approximately 4 hours (see diagram below). Input DNA is first subjected to targeted PCR to amplify the V3-V4 region of the DNA encoding 16S rRNA. The post-PCR reaction is then cleaned up using AMPure XP beads. Dual index primers are then added using a limited-cycle PCR. The indexed amplicons flanked by 5′ and 3′ barcoded adaptors are then cleaned using AMPure XP beads. The libraries are then ready for quantification, pooling and sequencing.

Details

Storage Conditions and Product Stability
Norgen’s 16S V3-V4 Library Prep Kit for Illumina is shipped as one kit box (for the 24 prep kit) or two sub-component kits (for the 96 prep kit). All kits should be stored at -20°C upon arrival.

All kit components should remain stable for at least 1 year when stored at the specified storage conditions.