Permagen’s most universal ring magnet plate. From Low elution PCR applications up to 2 mL deep well, the X96 has you covered. No need to purchase two separate plates anymore. Smaller inner ring magnet allows for volumes as low as 5 µL, larger outer magnet handles up to 2 mL deep well 

ANSI/ SBS Footprint (127.75mm x 85.50mm) to fit into any automated liquid handling robot on bottom, SBS/ SLAS fit on top to accept any microplate

Integrated Cushion base for maximum recovery. Helps aid in set-up, robot positioning inconsistencies, and labware consumable differences 

Features include solid aluminum alloy construction and hard coat anodized finish for years of trouble-free use, and compatible with any magnetic beads

SKU

A011623

A011623KT (Kit for 0.2 ml tubes)

Features

• ANSI/ SBS footprint
• Dual ring magnet technology 
• Cushion base for added benefits
• Solid Aluminum alloy design
• Precision manufactured for more consistent results
• Fast magnetic bead separations
• Made in USA 
• For Research use only

Compatibility

• Round bottom plates 
• Pyramid bottom plates 
• Flat Bottom plates
• Deep well plates
• Full-, Semi-, and un-Skirted PCR Plates
• Any magnetic beads
• Any common liquid handlers i.e. Tecan®, Opentrons®, Hamilton®, Agilent®, Beckman®, etc.

Volumes

Maximum – 2 mL (Deep Well)

Minimum – 5 µL (PCR Plate)

Permagen’s most universal ring magnet plate. From Low elution PCR applications up to 2 mL deep well, the X96 has you covered. No need to purchase two separate plates anymore. Smaller inner ring magnet allows for volumes as low as 5 µL, larger outer magnet handles up to 2 mL deep well

Introduction

This product is suitable for extracting RNA from anticoagulant blood, lymphocytes, buffy coat, bone marrow, cultured cells and other samples.

Details

Specifications

Features	Specifications
Main Functions	Isolation total RNA from 1-1.5ml whole blood, 0.5-1ml bone marrow, buffy coat
Applications	RT-PCR, cDNA synthesis, second generation sequencing
Purification method	Polydisperse magnetic beads
Purification technology	Magnetic beads technology
Process method	Manual or automatic
Sample type	anticoagulant blood, lymphocytes, buffy coat, bone marrow, cultured cells
Sample amount	1-1.5ml whole blood, 0.5-1ml bone marrow
Yield	1-30μg

Principle

This product is suitable for extracting RNA from anticoagulant blood, lymphocytes, buffy coat, bone marrow, cultured cells and other samples. This product is based on the purification method of high binding magnetic particles. The sample is lysed and digested by lysis buffer and protease, and RNA/DNA is released into the lysis buffer. Add binding solution and magnetic particles to adsorb RNA/DNA, while proteins are not adsorbed and removed. The particles adsorbed with DNA/RNA are washed with washing buffer to remove proteins and other impurities, then washed with ethanol to remove salt, and finally digested with DNase to remove DNA. RNA is recovered by adding binding solution, and finally the RNA is eluted with low salt buffer. The eluted RNA can be directly used for experiments such as RT-PCR,NGS and virus detection.

Advantages

• High purity – OD 260 / 280 :1.9-2.0, OD 260 / 230 :1.5-2.0
• Economy – less than 50% of the price of top brands
• Automatic – extraction without manual participation, saving time and effort
• High yield – nucleic acid recovery up to 95%
• General – samples include whole blood, serum, plasma, lymphocyte suspension, bone marrow, cultured cells, etc.

Kit Contents

Contents	R661101	R661102	R661103
Purification Times	48 Preps	96 Preps	480 preps
10 x RBC Lysis Buffer	50 ml	2 x 50 ml	4 x 100 ml
Proteinase K	12 mg	24 mg	120 mg
Protease Dissolve Buffer	1.8 ml	1.8 ml	10 ml
DNase I	600 μl	2 x 600 μl	10 x 600 µl
DNase Buffer	20 ml	30 ml	150 ml
MagPure Particles N	1.2 ml	2.5 ml	11 ml
Buffer RTL	30 ml	60 ml	300 ml
Buffer ALB2	40 ml	60 ml	300 ml
Buffer MW1*	22 ml	44 ml	220 ml
Buffer MW2*	20 ml	50 ml	2 x 100 ml
RNase Free Water	10 ml	20 ml	60 ml

Storage and Stability

DNase I should be shipped with ice pack or dry ice and stored at -20°C upon arrival. MagPure Particles N and Proteinase K should be stored at 2–8°C upon arrival. However, short-term storage(up to 8 weeks) at room temperature (15–25°C) does not affect their performance.The remaining kit components can be store at room temperature and are stable for up to 18 months under these conditions.

This product is suitable for extracting RNA from anticoagulant blood, lymphocytes, buffy coat, bone marrow, cultured cells and other samples.

Description 

Champion™ E. coli Transformation Kit provides an easy method for rapid preparation of chemically competent cells with high transformation efficiency from fresh culture, overnight culture, or even directly from bacterial colonies on the plate. The competent cell preparation method eliminates the requirement of time-wasting wash step. In addition, preparation of competent cells from overnight culture or directly from bacterial colonies provides flexibility to cloning experiment. The resultant competent cells can be immediately used or stored at -70°C for one year. This kit includes a specialized SMO-Broth™ medium and a unique Champion™ CC Buffer for culturing and preparing competent cells efficiently. Following the simple and quick competent cell preparation protocol from fresh culture, the transformation efficiency is typically ranged from 10⁸–10⁹ cfu/μg transformants/μg of pUC19 plasmid DNA, but varies depending on the E. coli strains. The resultant competent cells can be further transformed using time-saving transformation protocol, eliminating the requirement of heat-shock and recovery steps. 

Features

• Flexible– fresh culture, overnight culture, 4°C stored liquid culture or even colonies on agar plate can be used for transformation. 
• Fast and Easy– only few steps for preparation; suitable for time-saving transformation 
• High efficiency– up to 109 cfu/μg 
• Personalization– suitable for most E. coli strains

Kit Contents

					Component	Volume
					Champion™ CC Buffer	20 ml
					SMO-Broth™	100 ml x 2
					pUC19 Control Plasmid (10-4 μg/μl)	5 µl
					Instruction Manual	1
					Champion™ Competent Cell Preparation Card	1

Storage

4°C for 12 months

Champion™ E. coli Transformation Kit provides an easy method for rapid preparation of chemically competent cells with high transformation efficiency from fresh culture, overnight culture, or even directly from bacterial colonies on the plate. The competent cell preparation method eliminates the requirement of time-wasting wash step. In addition, preparation of competent cells from overnight culture or directly from bacterial colonies provides flexibility to cloning experiment. The resultant competent cells can be immediately used or stored at -70°C for one year. This kit includes a specialized SMO-Broth™ medium and a unique Champion™ CC Buffer for culturing and preparing competent cells efficiently. Following the simple and quick competent cell preparation protocol from fresh culture, the transformation efficiency is typically ranged from 108–109 cfu/μg transformants/μg of pUC19 plasmid DNA, but varies depending on the E. coli strains. The resultant competent cells can be further transformed using time-saving transformation protocol, eliminating the requirement of heat-shock and recovery steps.