Epstein-Barr Virus (EBV) TaqMan PCR Lyophilized Kits

Product advantages

1. The instantaneous centrifugal function rises to the maximum speed for about 6 seconds, allowing for rapid detachment of hanging droplets from the wall;

2. Faster acceleration and deceleration rate, required to complete the experiment in a shorter time;

3. Door cover protection, overspeed and imbalance detection system, capable of real-time monitoring of  the centrifugal process, ensuring the safe operation    of the  instrument; When the operation ends, an error occurs, or an imbalance occurs, the sound signal prompts, and the operation stops at the same time. The LCD displays the result code

Host Parameters

Product model	Mini-3
Input power supply	DC24V/2.75A
Input power	55W
Motor/Drive Method	DC24V brushless DC variable frequency motor
Display method	High brightness LCD screen
Effective centrifugal time	1-99min.1-59sec
speed	300~3000±15rpm
Speed step increase	10rpm
Maximum capacity	2 *96 well PCR plates/ELISA plates
MAX. RCF	608 xg
Misoperation/alarm failure	Sound prompt+display code
weight	3.9Kg
Noise	60dB (A)
Fastest acceleration time	<6s
Fastest deceleration time	<5s
Permissible ambient temperature/relative temperature	+5-40°C/80%

The micro porous plate centrifuge adopts international advanced design concepts and manufacturing technology, with a smooth and beautiful appearance, compact and stable structure, adhering to the advantages of high universality and easy operation. It also has the functional characteristics of “smooth start” and “smooth braking”; This micro porous plate centrifuge is very suitable for 96 or 384 well and small capacity micro porous plates, as well as various standard PCR micro porous plates with or without skirts .

Overview

Peelable heat sealing film which is made using gas permeable grid lacquer paper. This seal is suitable for insect studies and seed storage.

• Heat sealing is a quick and cost effective method of plate sealing
• Our Gas PermASeal II Heat Seal is a paper with a grid lacquer coating which is porous and gas permeable
• The Gas PermeASeal II Heat Seal is compatible with polypropylene, polyethylene, polystyrene and cyclic olefin copolymer (COC) plates
• The seal can be removed by peeling, or it can be pierced with a pipette tip manually or using a liquid handling robot
• The Seal has a temperature range from -20°C to 80°C
• It can be used for insect and seed storage, as it enables gas exchange, whilst providing an inert surface with no adhesive to interfere with the well contents
• This seal is available as sheets, for use with manual and semi-automated sealers, such as our HeatASeal 500 Sealing Machine
• Also available in roll format compatible with specified automated heat sealers, such as our Wasp or Chameleon XT

Peelable heat sealing film which is made using gas permeable grid lacquer paper. This seal is suitable for insect studies and seed storage.

Overview

• Isolate high quality, high yield plasmid DNA
• Plasmid DNA is ready for various downstream applications including restriction digestion, bacterial transformation, sequencing and more
• Available in 4 formats: MiniPrep, MiniPrep (Magnetic Bead System), 96-Well MiniPrep (Magnetic Bead System), and MaxiPrep

These kits are designed for the rapid preparation of plasmid DNA from Escherichia coli.

Plasmid MiniPrep Kit

This kit is designed for the rapid preparation of plasmid DNA from small cultures of Escherichia coli using convenient spin columns. The plasmid DNA is preferentially purified from other cellular components such as genomic DNA and RNA. This kit is able to purify plasmids up to 13,000 bp in size, and the typical purification yield is up to 20 μg from 1.5 mL of bacterial culture. Purified DNA is of excellent quality for transformation, restriction enzyme digestion, sequencing and more. Also available in a 96-well format.

Plasmid MiniPrep Kit (Magnetic Bead System and High Throughput Magnetic Bead System)

Norgen’s Plasmid MiniPrep Kit (Magnetic Bead System) is designed for the rapid preparation of plasmid DNA from small batch cultures of Escherichia coli. Norgen’s magnetic beads bind DNA under optimized salt concentrations and release the bound DNA under low salt and slightly alkali conditions. The plasmid DNA is preferentially purified from other cellular components such as genomic DNA and RNA. The purified plasmids are fully digestible with all restriction enzymes tested, and are completely compatible with real-time PCR and NGS.

Norgen’s Plasmid MiniPrep Kit (Magnetic Bead System) is also available in a 96-well (HT) format for high throughput applications. Purification with the 96-well plates can be integrated with a robotic automation system.

Plasmid MaxiPrep Kit

This kit is designed for the rapid spin column preparation of plasmid DNA from up to 100 mL of Escherichia coli cultures. The kit allows for the isolation of plasmid DNA with final endotoxin levels of 0.1 EU/µg of DNA or less. The kit is able to purify plasmids up to 13,000 bp in size, and typical yields from a 100 mL culture for a high copy number plasmid are between 0.4 and 1.0 mg. The purified DNA is fully digestible with all restriction enzymes tested, and is completely compatible with manual or automated sequencing to achieve 95-100% accuracy.

Details

Supporting Data

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Kit Specifications
Size of Plasmids Purified	Up to 13,000 bp
Average Yield from 1.5 mL of Culture	Up to 20 μg
Time to Complete 10 Purifications	30 minutes (Cat. 60300) 45 minutes (Cat. 63000)

Storage Conditions and Product Stability
All solutions should be kept tightly sealed and stored at room temperature. This kit is stable for 1 year from the date of shipment. The RNase should be stored at -20°C upon arrival. The Resuspension Solution A should be stored at 4°C upon addition of RNase enzyme.

Component	Cat. 13300 (50 preps)	Cat. 46400 (250 preps)	Cat. 46500 (4 preps)	Cat. 46600 (20 preps)	Cat. 60300 (50 preps)	Cat. 63000 (192 preps)
Resuspension Solution AZ	12 mL	60 mL	20 mL	100 mL	12 mL	2 x 20 mL
Lysis Buffer N	40 mL	80 mL	40 mL	2 x 80 mL	40 mL	2 x 40 mL
Buffer TN	20 mL	130 mL	55 mL	2 x 130 mL	20 mL	1 x 55 mL 1 x 20 mL
Wash Solution E	12 mL	2 x 18 mL	–	–	–	–
Elution Buffer K	8 mL	30 mL	–	–	8 mL	2 x 8 mL
Wash Solution J	–	–	25 mL	3 x 25 mL	–	–
Elution Buffer J	–	–	24 mL	120 mL	–	–
RNase A	1 vial	1 vial	1 vial	1 vial	1 vial	1 vial
Magnetic Bead Suspension	–	–	–	–	1 x 1.1 mL	4 x 1.1 mL
Spin Columns	50	250	–	–	–	–
Collection Tubes	50	250	–	–	–	–
DNA Maxi Spin Columns with Collection Tubes (Clear ring in column)	–	–	4	20	–	–
Maxi Spin Filter Columns with Collection Tubes (Grey ring in column)	–	–	4	20	–	–
96-Well Plate	–	–	–	–	–	2
Elution Tubes (1.7 mL)	50	250	–	–	50	–
Elution Tubes (50 mL)	–	–	4	20
96-Well Elution Plate	–	–	–	–	–	2
Adhesive Tape	–	–	–	–	–	2
Product Insert	1	1	1	1	1	1