The Flex caddy allows the Heater-Shaker GEN1 to be installed on the Opentrons Flex deck and sit below the deck, enabling below-deck cable routing and labware placed on top of modules to remain closer to the deck surface. The calibration adapter is used to calibrate the location of the Heater-Shaker. Caddies and calibration adapters are specific to the type of module.
Note: This item does not include the Heater-Shaker. The module is available with or without the Flex Caddy and Calibration tool: Heater-Shaker Module GEN1
Detail
The Flex caddy allows the Heater-Shaker GEN1 to be installed on the Opentrons Flex deck and sit below the deck, enabling below-deck cable routing and labware placed on top of modules to remain closer to the deck surface. The calibration adapter is used to calibrate the location of the Heater-Shaker. Caddies and calibration adapters are specific to the type of module.
Note: This item does not include the Heater-Shaker. The module is available with or without the Flex Caddy and Calibration tool: Heater-Shaker Module GEN1
Actin is part of the cytoskeletal system of all cell types. Smooth Muscle Actin is found in myofibroblasts and myoepithelium, but not in cardiac and skeletal muscles. Labelling of smooth muscle actin in concert with muscle specific actin staining can allow for differentiation between rhabdomyosarcoma and leiomyosarcoma, as Muscle specific actin is found in rhabdomyoblasts, while smooth muscle actin is found in leiomyosarcomas.
Plasma/Serum Exosome Purification and RNA Isolation Kits
Product Info
Document
Product Info
Overview
Purification and enrichment of intact plasma/serum exosomes for functional studies
Isolate all sizes of RNA, including microRNA, irrespective of size or GC content, without bias
Versatile plasma/serum input volume
No phenol extractions, Proteinase K treatment, nor carrier RNA required
No time-consuming ultracentrifugation, filtration nor special syringes required
No precipitation reagents, nor overnight incubation required
Compatible with plasma/serum from most species
Pure exosomes are purified and are free from any other RNA-binding proteins
Purification is based on spin column chromatography that uses Norgen’s proprietary resin
The purified RNA is suitable for a variety of downstream applications, including Small RNA Sequencing. Find out more information on Norgen’s NGS services
Norgen’s Plasma/Serum Exosome and RNA Isolation Kits constitute all-in-one systems for the purification of exosomes and the sequential isolation of exosomal RNA from different plasma/serum sample volumes. The purification is based on spin column chromatography that employs Norgen’s proprietary resin. These kits are designed to isolate all sizes of RNA, including microRNA, irrespective of size or GC content, without bias. These kits provide a clear advantage over other available kits in that they do not require any special instrumentation, protein precipitation reagents, extension tubes, phenol/chloroform or protease treatments. The RNA isolated from the purified exosomes is free from any protein-bound circulating RNA and is of the highest integrity. The purified RNA can be used in a number of downstream applications including real time PCR, reverse transcription PCR, Northern blotting, RNase protection and primer extension, and expression array assays.
Norgen’s Plasma/Serum Exosome and RNA Isolation Mini Kit
For sample volumes ranging from 50 µL to 1 mL. This kit allows the user to elute into a flexible elution volume ranging from 50 µL to 100 µL.
Norgen’s Plasma/Serum Exosome and RNA Isolation Midi Kit
For sample volumes ranging from 1 mL to 4 mL. This kit allows the user to elute into a flexible elution volume ranging from 50 µL to 100 µL.
Norgen’s Plasma/Serum Exosome and RNA Isolation Maxi Kit
For sample volumes ranging from 4 mL to 10 mL. This kit allows the user to elute into a flexible elution volume ranging from 50 µL to 100 µL.
All sizes, including miRNA and small RNA (< 200 nt)
Elution Volume
50-100 μL
Time to Complete 10 Purifications
35 – 40 minutes
Average Yields
Variable depending on specimen
*Please check page 4 of the product insert for the average yields and the common RNA quantification methods
Storage Conditions and Product Stability All solutions should be kept tightly sealed and stored at room temperature. This kit is stable for 2 years after the date of shipment. It is recommended to warm Lysis Buffer A for 20 minutes at 60°C if any salt precipitation is observed.
Important Note This kit is suitable for the purification of exosomes from fresh or frozen serum or plasma prepared from blood collected on either EDTA or Citrate. Plasma samples prepared from blood collected on heparin should not be used as heparin can significantly interfere with many downstream applications such as RT-PCR.
DBCO-NHS Ester is a very popular amine-reactive click chemistry reagent. It can be used to modify an amine-containing molecule in organic solvents (limited solubility in aqueous media). It reacts with primary amines such as the side chain of lysine residues or aminosilane-coated surfaces at neutral or slightly basic pH to form covalent bonds. The low mass weight will add minimal spacer to modified molecules. DBCO is commonly used for copper-free Click Chemistry reactions. Reagent grade, for research purpose. Please contact us for GMP-grade inquiries.
Document
DBCO-NHS Ester is a very popular amine-reactive click chemistry reagent. It can be used to modify an amine-containing molecule in organic solvents (limited solubility in aqueous media). It reacts with primary amines such as the side chain of lysine residues or aminosilane-coated surfaces at neutral or slightly basic pH to form covalent bonds. The low mass weight will add minimal spacer to modified molecules. DBCO is commonly used for copper-free Click Chemistry reactions. Reagent grade, for research purpose. Please contact us for GMP-grade inquiries.
