Introduction

This kit is used for extracting total viral nucleic acid from non-cell/low cell content biological samples such as body fluid, serum, plasma, immersion solution, tissue homogenate supernatant, culture supernatant, etc., the extracted products can be used for clinical in vitro detection.

Details

Specifications

Features	Specifications
Main Functions	Extract viral RNA/DNA from 200μl plasma/serum samples
Applications	RT-PCR，PCR，NGS
Products	Viral total nucleic acid, body cell total nucleic acid, negative bacterial DNA
Purification method	Mini spin column
Purification technology	Silica technology
Process method	Manual (centrifugation or vacuum)
Sample type	Whole blood, plasma, serum, soaking solution and tissue homogenate supernatant
Sample amount	200μl
Yield	2-10μg
Elution volume	≥30μl
Time per run	≤30 minutes
Liquid carrying volume per column	800μl
Binding yield of column	100μg

Principle

Hipure silica gel column is based on glass fiber filter membrane with high binding force. Under the condition of high concentration of ionizing agent (such as guanidine hydrochloride or guanidine isothiocyanate), the filter membrane can adsorb nucleic acid through hydrogen bond and electrostatic, while protein and other impurities are not adsorbed and removed. The filter membrane adsorbed with nucleic acid is washed to remove the residual protein and salt. Finally, the nucleic acid adsorbed on the filter membrane can be washed out with low salt buffer (such as buffer TE) or water. The obtained nucleic acid has high purity and can be directly used in various downstream experiments.

Advantages

• Fast – several samples can be extracted in 20 minutes by column method
• High quality – high purity total RNA can be directly used in various sensitive downstream applications
• Safe – no phenol chloroform extraction required
• Sensitive – DNA/RNA can be recovered at the level of PG
• High yield – carrier RNA contained in the product maximize the recovery of trace nucleic acid

Kit Contents

Contents	IVD4173
Purification Times	100 Preps
HiPure Viral Mini Column	100
2ml Collection Tubes	200
PK/Carrier RNA	50 mg
Protease Dissolve Buffer	5 ml
Buffer AL	30 ml
Buffer MW1	44 ml
Buffer MW2	50 ml
RNase Free Water	15 ml

Storage and Stability

This kit is shipped and stored at room temperature and is valid for 12 months.

Experiment Data

This kit is used for extracting total viral nucleic acid from non-cell/low cell content biological samples such as body fluid, serum, plasma, immersion solution, tissue homogenate supernatant, culture supernatant, etc., the extracted products can be used for clinical in vitro detection.

Overview

• Clean-up & concentrate total RNA (including miRNA) in minutes
• Clean-up & concentrate RNA from TRIzol^®, TRI Reagent^®, etc
• Clean-up RNA from contaminants including enzymes, primers, nucleotides
• Rapid spin-column protocol, elute in 20 µL
• 96-well format for high throughput processing & Micro-Elute spin column formats for 8 µL are available
• Purified RNA is suitable for a variety of downstream applications, including Small RNA Sequencing. Find out more information on Norgen’s NGS services
• Purification is based on spin column chromatography that uses Norgen’s proprietary resin separation matrix

These kits are designed to clean-up and concentrate RNA (including miRNA) from TRIzol^® and TRI Reagent^®, enzymatic reactions, in vitro transcription, labelling reactions, etc. These are robust kits for all clean-up and concentration purposes for up to 35 µg of RNA in solution. The purified RNA is of the highest purity and integrity, and can be used in a number of downstream applications. These kits purify all sizes of RNA, from large mRNA and ribosomal RNA down to miRNA, siRNA and lncRNA.

RNA Clean-Up and Concentration Kit (Spin Column)

Norgen’s RNA Clean-Up and Concentration Kit provides a rapid method for the purification, cleanup and concentration of up to 35 μg of RNA isolated using different methods including phenol/guanidine-based protocols, and from various upstream enzymatic reactions such as DNase treatment and labeling. The kit elutes RNA in 20 µL. Complete 10 purifications in 20 minutes.

RNA Clean-Up and Concentration 96-Well Kit (High Throughput)

Norgen’s RNA Clean-Up and Concentration 96-Well Kit provides a rapid method for the purification, cleanup and concentration of up to 50 μg of RNA isolated using different methods including phenol/guanidine-based protocols, and from various upstream enzymatic reactions such as DNase treatment and labeling. The kit elutes RNA in 75 µL. Complete 10 purifications in 30 minutes.

RNA Clean-Up and Concentration Micro-Elute Kit (Micro-Elute)

Norgen’s RNA Clean-Up and Concentration Micro-Elution Kit provides a rapid method for the purification, cleanup and concentration of up to 10 μg of RNA isolated using different methods including phenol/guanidine-based protocols, and from various upstream enzymatic reactions such as DNase treatment, labeling and in vitro transcription. This kit is made for eluting RNA in smaller volumes of 8 µL for all types of downstream applications, for the highest concentration of RNA sample. Complete 10 purifications in 20 minutes.

Protocol

Details

Supporting Data

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Kit Specifications (Spin Column)
Maximum Column Binding Capacity	35 μg
Size of RNA Purified	All sizes, including small RNA (<200 nt)
Maximum Amount of Starting Material	35 μg of RNA
Minimum Elution Volume	20 μL
Time to Complete 10 Purifications	20 minutes
Average Recovery	≥ 90%

Storage Conditions and Product Stability
All solutions should be kept tightly sealed and stored at room temperature. This kit is stable for 2 years after the date of shipment.

Component	Cat. 23600 (50 preps)	Cat. 43200 (100 preps)	Cat. 25100 (192 preps)	Cat. 61000 (50 preps)
Buffer RL	40 mL	2 x 40 mL	2 x 40 mL	40 mL
Wash Solution A	38 mL	2 x 38 mL	2 x 38 mL	38 mL
Elution Solution A	6 mL	2 x 6 mL	2 x 20 mL	6 mL
Column Activation Solution	–	–	–	30 mL
Micro Spin Columns	50	100	–	–
Micro-Elute RNA Spin Columns	–	–	–	50
96-Well Plate	–	–	2	–
Adhesive Tape	–	–	4	–
Collection Tubes	50	100	–	50
96-Well Collection Plate	–	–	2	–
Elution Tubes (1.7 mL)	50	100	–	50
96-Well Elution Plate	–	–	2	–
Product Insert	1	1	1	1

Name of Product

Swab Detection Buffer

Catalog Number

MGSDB

Short Info

Extractionbuffer for swabs

Method/Platform

Buffer for lateral flow assay

Range/Assay Sensivity

Test Principle

The Milenia swab PCR-System allows the direct detection of obligate spoilers from a swab. Areas of poor Hygiene and hotspots of Biofilm Formation can be quickly identified and monitored.

Brief Instructions

Storage

Components

Name of Product
Swab Detection Buffer
Catalog Number
MGSDB
Short Info
Extractionbuffer for swabs

Method/Platform
Buffer for lateral flow assay
Range/Assay Sensivity
Test Principle
The Milenia swab PCR-System allows the direct detection of obligate spoilers from a swab. Areas of poor Hygiene and hotspots of Biofilm Formation can be quickly identified and monitored.