Floor Type High Speed & Low Speed Universal Refrigerated Centrifuge

Features

1. Brushless frequency motor, in great torque, free maintenance, no powder pollution, quick in speed up and down.

2.Imported compressors fluorine free,double cycle cooling, cold and hot alternating easily, free environment pollution and precise in temperature control

3.Microprocessor control, digital display which indicates the temperature, speed, time, RCF in operation, speed raising and reducing quickly, operate simply. Running parameter can be edited.

4. 10 kinds of program stored in the memory, 10 kinds of accelerating and decelerating speed for your choice.

5. Automatically electric lid lock, over speed, over temperature protection and imbalance protection.

6.High quality steel centrifuge body , stainless steel chamber, built-in stainless steel explosion-proof protect inner sleeve , 3 tiers protection,safe and reliable.

7. Rotor is connected to spindle by specialized taper sleeve, loading simple and quick, no direction.

Detail

GL20 Technical Parameter：

Max. Speed	21000rpm
Max. RCF	30910×g
Max. Capacity	4×800ml
Time Range	1~9h59min
RPM/RCF Convert	Yes
Noise (dB)	≤ 60
Temperature	-20℃~40℃
Acc/Dec	10 Kinds
Speed Accuracy	±20r/min
Temperature Accuracy	±1℃
Voltage(V/Hz)	AC 220V/110V 50HZ/60HZ
Size (W x D x Hmm)	680×540×830mm
Net Weight(Kg)	170KG
Certificates	CE,ISO & Calibration report are available

Matched Rotors for GL20

Order No	Rotor	Rotor Max Speed(r/min)	Volume(ml)	RCF(×g)
G20-1	Swing Rotor	4000	4×800ml	3450
G20-2	Microplate rotor	4000	4×4×96 well	2940
G20-3	Microplate rotor	4000	2×4×96 well	3210
G20-4	Microplate rotor	4000	2×3×48 well	2300
G20-5	Swing rotor	4000	4×40×7ml vacuum tube	3270
G20-6		4000	4×40×5ml vacuum tube	2890
G20-7		4000	4×28×10ml vacuum tube	3130
G20-8	Swing Rotor	4000	Fat bottle	3830
G20-9	Fixed Rotor	16000	4×8PCR	15760
G20-10	Fixed Rotor	15000	6×8PCR	21420
G20-11	Fixed Rotor	16000	8×8PCR	17480
G20-12	Fixed Rotor	15000	12×8PCR	22930
G20-13	Fixed Rotor	15000	40×0.5ml	22920
G20-14	Fixed Rotor	21000	12×1.5ml	30910
G20-15	Fixed Rotor	16000	24×1.5ml	23440
G20-16	Fixed Rotor	14000	30×1.5ml	20800
G20-17	Fixed Rotor	13000	48×1.5ml	17930
G20-18	Fixed Rotor	16000	16×5ml	22020
G20-19	Fixed Rotor	16000	6×10ml	21500
G20-20	Fixed Rotor	15000	12×10ml	22680
G20-21	Fixed Rotor	13000	16×10ml	19490
G20-22	Fixed Rotor	13000	8×15ml	17790
G20-23	Fixed Rotor	11000	12×15ml	14330
G20-24	Fixed Rotor	5000	24×15ml	3500
G20-25	Fixed Rotor	5000	30×15ml	3830
G20-26	Fixed Rotor	14000	6×30ml	19060
G20-27	Fixed Rotor	13000	6×50ml(conical)	18840
G20-28	Fixed Rotor	13000	6×50ml(round)	18730
G20-29	Fixed Rotor	5000	12×50ml	3860
G20-30	Fixed Rotor	4000	24×50ml	2970
G20-31	Fixed Rotor	13000	4×85ml	18940
G20-32	Fixed Rotor	12000	4×100ml	14850
G20-33	Fixed Rotor	10000	6×100ml	11380
G20-34	Fixed Rotor	4000	12×100ml	2970
G20-35	Fixed Rotor	12000	24 capillaries	15800
G20-36	Swing Rotor	15000	4×5ml	19920
G20-37	Vertical rotor	16000	16×5ml	16450
G20-38	Vertical rotor	14000	8×30ml	19750

Other Products

Plant/Fungi Total RNA Purification Kit

Product Info

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Product Info

Overview

Extract total RNA, including virus & viroid RNA
Robust lysis buffer is well-suited to even challenging samples such as pine needle, grape leaf, etc
Isolate total RNA (including microRNA) without phenol
Isolated RNA is of high quality, integrity and diversity
Also available in 96-well format for high throughput applications
Purification is based on spin column chromatography that uses Norgen’s proprietary resin separation matrix

Norgen’s Plant/Fungi Total RNA Purification Kit provides a rapid method for the isolation and purification of total RNA, including virus and viroid RNA, from a wide range of plants. Total RNA can be purified from fresh or frozen plant tissues, plant cells or filamentous fungi samples using this kit. All sizes of RNA are purified, including microRNA (miRNA) . The procedure is rapid and convenient.

The RNA is purified without the use of phenol or chloroform. The purified RNA is of the highest quality, and can be used in a number of downstream applications including real time PCR, reverse transcription PCR, Northern blotting, RNase protection and primer extension, and expression array assays.

Norgen’s Plant/Fungi Total RNA Purification Kit is also available in a 96-well (High Throughput) format for high throughput applications. Purification with the 96-well plates can be performed using either a vacuum manifold or centrifugation.

Details

Supporting Data

Figure 1 / 4

Plant/Fungi Total RNA Purification Kit Figure 1

Plant/Fungi Total RNA Purification Kit Figure 1

Plant/Fungi Total RNA Purification Kit Figure 2

Plant/Fungi Total RNA Purification Kit Figure 3

Plant/Fungi Total RNA Purification Kit Figure 4

Click for expanded view

Kit Specifications – Spin Column
Maximum Column Binding Capacity	50 μg
Maximum Column Loading Volume	650 μL
Size of RNA Purified	All sizes, including small RNA (< 200 nt)
Maximum Amount of Starting Material: Plant Tissues Plant Cells Fungi	50 mg 1 x 10⁶ cells 50 mg (wet weight)
Average Yield* 50 mg Tomato Leaves 50 mg Tobacco Leaves 50 mg Plum Leaves 50 mg Grape Leaves 50 mg Peach Leaves	60 μg 60 μg 32 μg 35 μg 30 μg
Time to Complete 10 Purifications	30 minutes

* Yield will vary depending on the type of sample processed.

* Yield will vary depending on the type of sample processed.

Storage Conditions and Product Stability
All solutions should be kept tightly sealed and stored at room temperature. This kit is stable for 2 years after the date of shipment.

Select Plants and Fungi Tested with the Norgen Plant/Fungi Total RNA Purification Kits

Plants

Tobacco (Nicotiana tabacum)
Tomato (Lycopersicon esculentum)
Pepper (Capsicum annuum)
Potato (Solanum tuberosum)
Arabidopsis thaliana1
Peach (Prunus persica)
Apple (Malus sp.)
Pear (Pyrus sp.)
Grape vine (Vitis sp.)
Plum (Prunus sp.)
Palm (Arecaceae)
Pine needle (Pinaceae)
Strawberry
Raspberry
Blackberry
Herbs
Persimmon (Ebenaceae)
Potato tuber (Solanum)
Plum fruit
Citrus
Vanilla bean
Cotton (Gossypium)
Mangrove
Chrysanthemum
Grape berry skin
Kiwi leaves
Peach (fruits and flowers)
Soy bean (legume)
Eastern White Red Cedar
Corn leaves
Cucumber leaves

Fungi

Aspergillus niger
Mucor racemosus
Cladosporium cladosporioides
Fusarium oxysporum
Penicillium sp.
Botrytis cinerea (Botryotinia fuckeliana)
Pichia sp.
Rhizopus oryzae
Alternaria tenuissima

Component	Cat. 25800 (50 preps)	Cat. 31350 (100 preps)	Cat. 25850 (250 preps)	Cat. 31900 (192 preps)
Lysis Buffer C	60 mL	1 x 30 mL, 1 x 60 mL	3 x 60 mL	2 x 60 mL
Wash Solution A	38 mL	38 mL	1 x 18 mL, 2 x 38 mL	2 x 38 mL
Elution Solution A	6 mL	6 mL	20 mL	20 mL
Filter Columns	50	100	250	–
Spin Columns	50	100	250	–
96-Well Plate	–	–	–	2
Adhesive Tape	–	–	–	4
Collection Tubes	100	200	500	–
96-Well Collection Plate	–	–	–	2
Elution Tubes (1.7 mL)	50	100	250	–
96-Well Elution Plate	–	–	–	2
Product Insert	1	1	1	1

Document

DBCO-PEG6-acid

Product Info

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Product Info

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Plasmid DNA Kits

Product Info

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Product Info

Overview

Isolate high quality, high yield plasmid DNA
Plasmid DNA is ready for various downstream applications including restriction digestion, bacterial transformation, sequencing and more
Available in 4 formats: MiniPrep, MiniPrep (Magnetic Bead System), 96-Well MiniPrep (Magnetic Bead System), and MaxiPrep

These kits are designed for the rapid preparation of plasmid DNA from Escherichia coli.

Plasmid MiniPrep Kit

This kit is designed for the rapid preparation of plasmid DNA from small cultures of Escherichia coli using convenient spin columns. The plasmid DNA is preferentially purified from other cellular components such as genomic DNA and RNA. This kit is able to purify plasmids up to 13,000 bp in size, and the typical purification yield is up to 20 μg from 1.5 mL of bacterial culture. Purified DNA is of excellent quality for transformation, restriction enzyme digestion, sequencing and more. Also available in a 96-well format.

Plasmid MiniPrep Kit (Magnetic Bead System and High Throughput Magnetic Bead System)

Norgen’s Plasmid MiniPrep Kit (Magnetic Bead System) is designed for the rapid preparation of plasmid DNA from small batch cultures of Escherichia coli. Norgen’s magnetic beads bind DNA under optimized salt concentrations and release the bound DNA under low salt and slightly alkali conditions. The plasmid DNA is preferentially purified from other cellular components such as genomic DNA and RNA. The purified plasmids are fully digestible with all restriction enzymes tested, and are completely compatible with real-time PCR and NGS.

Norgen’s Plasmid MiniPrep Kit (Magnetic Bead System) is also available in a 96-well (HT) format for high throughput applications. Purification with the 96-well plates can be integrated with a robotic automation system.

Plasmid MaxiPrep Kit

This kit is designed for the rapid spin column preparation of plasmid DNA from up to 100 mL of Escherichia coli cultures. The kit allows for the isolation of plasmid DNA with final endotoxin levels of 0.1 EU/µg of DNA or less. The kit is able to purify plasmids up to 13,000 bp in size, and typical yields from a 100 mL culture for a high copy number plasmid are between 0.4 and 1.0 mg. The purified DNA is fully digestible with all restriction enzymes tested, and is completely compatible with manual or automated sequencing to achieve 95-100% accuracy.

Details

Supporting Data

Figure 1 / 5

Figure 1. High Yield DNA from Small Cultures

Figure 1. High Yield DNA from Small Cultures

Figure 2. High Accuracy Sequencing

Figure 3. High Quality of Plasmid DNA

Figure 4. High DNA Yield

Figure 5. Full Compatibility with Digests

Click for expanded view

Kit Specifications
Column Binding Capacity	1.5 mg
Average Yield from 100 mL Culture*	0.4 – 1.0 mg
Final Endotoxin Levels	< 0.1 EU/µg DNA
Time to Complete 4 Purifications	1.5 hours
Size of Plasmids Purified	Up to 13,000 bp

* Depends on high-copy DNA and low copy DNA

Storage Conditions and Product Stability
All solutions should be kept tightly sealed and stored at room temperature. The RNase vial should be stored at -20°C upon arrival. Once RNase has been added to the Resuspension Solution AZ the solution should be stored at 4°C. This kit is stable for 1 year after its date of shipment.

Component	Cat. 13300 (50 preps)	Cat. 46400 (250 preps)	Cat. 46500 (4 preps)	Cat. 46600 (20 preps)	Cat. 60300 (50 preps)	Cat. 63000 (192 preps)
Resuspension Solution AZ	12 mL	60 mL	20 mL	100 mL	12 mL	2 x 20 mL
Lysis Buffer N	40 mL	80 mL	40 mL	2 x 80 mL	40 mL	2 x 40 mL
Buffer TN	20 mL	130 mL	55 mL	2 x 130 mL	20 mL	1 x 55 mL 1 x 20 mL
Wash Solution E	12 mL	2 x 18 mL	–	–	–	–
Elution Buffer K	8 mL	30 mL	–	–	8 mL	2 x 8 mL
Wash Solution J	–	–	25 mL	3 x 25 mL	–	–
Elution Buffer J	–	–	24 mL	120 mL	–	–
RNase A	1 vial	1 vial	1 vial	1 vial	1 vial	1 vial
Magnetic Bead Suspension	–	–	–	–	1 x 1.1 mL	4 x 1.1 mL
Spin Columns	50	250	–	–	–	–
Collection Tubes	50	250	–	–	–	–
DNA Maxi Spin Columns with Collection Tubes (Clear ring in column)	–	–	4	20	–	–
Maxi Spin Filter Columns with Collection Tubes (Grey ring in column)	–	–	4	20	–	–
96-Well Plate	–	–	–	–	–	2
Elution Tubes (1.7 mL)	50	250	–	–	50	–
Elution Tubes (50 mL)	–	–	4	20
96-Well Elution Plate	–	–	–	–	–	2
Adhesive Tape	–	–	–	–	–	2
Product Insert	1	1	1	1	1	1

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