Attogene test uses the property of formaldehyde to react with the Formaldehyde Reaction Powder (FRP) to form a purple-red tetrazine. The formaldehyde concentration is measured by visual comparison of the reaction with the color scale derived from the Color Card.
Formaldehyde is an organic compound with the formula CH2O. It is mainly used in the production of industrial resins but has been found to be used as a preservative, disinfectant and biocide. Because of its toxicity and volatility formaldehyde poses a significant risk to human health. Attogene test uses the property of formaldehyde to react with the Formaldehyde Reaction Powder (FRP) to form a purple-red tetrazine. The formaldehyde concentration is measured by visual comparison of the reaction with the color scale derived from the Color Card.
Measuring range / color- Number of scale graduation
EXTRAClean Cell Culture Media Exosome Purification and RNA Isolation Kits
Product Info
Document
Product Info
Overview
Ensure optimal results for sensitive applications like NGS
Up to a tenfold increase in microRNA mapping during sequencing runs to reduce costs
Purification and enrichment of intact cell culture media exosomes for functional studies
No phenol extractions, Proteinase K treatment, nor carrier RNA required
No time-consuming ultracentrifugation, filtration nor special syringes required
No precipitation reagents nor overnight incubation required
Pure exosomes are purified and are free-from any other RNA-binding proteins
Purification is based on EXTRAClean spin column chromatography that uses Norgen’s proprietary resin separation matrix
Norgen’s EXTRAClean Cell Culture Media Exosome Purification and RNA Isolation Kits constitute all-in-one systems for the purification of exosomes and the subsequent isolation of RNA from different cell culture media sample volumes. The purification is based on spin column chromatography that employs Norgen’s proprietary resin. The EXTRAClean columns undergo stringent processing and rigorous quality control measures to minimize contamination traces, ensuring optimal results for sensitive applications such as NGS. The kit is designed to isolate all sizes of RNA, including microRNA. The kit provides a clear advantage over other available kits in that it does not require any special instrumentation, protein precipitation reagents, extension tubes, phenol/chloroform or protease treatments. Moreover, the kits allows the user to elute into flexible elution volumes ranging from 50 μL to 100 μL. The purified RNA is free from any protein-bound circulating RNA and is of the highest integrity. The purified RNA can be used in a number of downstream applications including real time PCR, Sequencing based applications, reverse transcription PCR, Northern blotting, RNase protection and primer extension, and expression array assays.
All sizes, including miRNA and small RNA (< 200 nt)
Elution Volume
50-100 μL
Time to Complete 10 Purifications
40-45 minutes
Average Yields
Variable depending on specimen
Storage Conditions and Product Stability All buffers should be kept tightly sealed and stored at room temperature. This kit is stable for 2 years after the date of shipment. It is recommended to warm Lysis Buffer A for 20 minutes at 60°C if any salt precipitation is observed.
MagPure Circulating DNA Mini Kit is designed for purification of high quality circulating DNA (cfDNA) fromcell-free body fluids (such as plasma, serum). The purified DNA is suitable for direct use in downstream applications such as PCR, real-time PCR, biochip analysis and NGS.
Details
Specifications
Features
Specifications
Main Functions
Isolation circulating DNA from 0.2-0.6ml plasma, serum, body fluids
Applications
qPCR, NGS, etc.
Purification technology
Magnetic beads technology
Process method
Manual or automatic
Sample type
Serum, plasma
Sample amount
0.2-0.6ml
Elution volume
≥30μl
Time per run
≤50 minutes
Principle
This product is based on the purification method of high binding magnetic particles. The sample is lysed and digested under the action of lysate and protease. DNA is released into the lysate. After adding magnetic particles and binding solution, DNA will be adsorbed on the surface of magnetic particles, and impurities such as proteins will be removed without adsorption. The adsorbed particles were washed with washing solution to remove proteins and impurities, washed with ethanol to remove salts, and finally DNA was eluted by elution buffer.
Advantages
Economy – less than 50% of the price of Qiagen and other imported products
Automatic – without labour
Kit Contents
Contents
IVD5432
Purification Times
200
MagPure Particles G
14 ml
Carrier RNA
310 μg
Proteinase K
180 mg
Protease Dissolve Buffer
10 ml
Buffer MLK
250 ml
Buffer MAW1
250 ml
Buffer MW2*
2 x 50 ml
Elution Buffer
60 ml
Contents
IVD5432-F-96A
IVD5432-F-96B
IVD5432-F-96C
Sample amount
200~350μl
400~700μl
Carrier RNA
110 μg
110 μg
Proteinase K
50 mg
100 mg
Protease Dissolve Buffer
5 ml
6 ml
Elution Buffer
15 ml
15 ml
Tip
1
1
Sample Plate A
500μl Buffer MLK
500μl Buffer MLK
Sample Plate B
/
500μl Buffer MLK
Wash Plate 1
700μl Buffer MAW1
700μl Buffer MAW1
Wash Plate 2
25μl Buffer MPG2700μl Buffer MW2
25μl Buffer MPG2700μl Buffer MW2
Wash Plate 3
700μl Buffer MW2
700μl Buffer MW2
Elution Plate
/
/
Contents
IVD5432-TL-06A
IVD5432-TL-06B
IVD5432-TL-06C
Sample amount
300~350μl
600~700μl
900~1050μL
Carrier RNA
310 μg
310 μg
310 μg
Proteinase K
50 mg
100 mg
150 mg
Protease Dissolve Buffer
6 ml
6 ml
10 ml
Elution Buffer
15 ml
15 ml
15 ml
DA-Tip
12
12
12
Row 1/7
600μl Buffer MLK
600μl Buffer MLK
600μl Buffer MLK
Row 2/8
/
600μl Buffer MLK
600μl Buffer MLK
Row 3/9
600μl Buffer MAW1
600μl Buffer MAW1
600μl Buffer MLK
Row 4/10
20μl Buffer MPG2600μl Buffer MW2
20μl Buffer MPG2600μl Buffer MW2
30μl Buffer MPG2900μl Buffer MAW1
Row 5/11
600μl Buffer MW2
600μl Buffer MW2
900μl Buffer MW2
Row 6/12
/
/
/
Storage and Stability
Proteinase K, Carrier RNA and MagPure Particles G should be stored at 2–8°C upon arrival. However, short-term storage (up to 12 weeks) at room temperature (15–25°C) does not affect their performance. Theremaining kit components can be stored dry at room temperature (15–25°C) and are stable for at least18 months under these conditions.The entire kit can be stored at 2–8°C, but in this case buffers should beredissolved before use. Make sure that all buffers are at room temperature when used.
Document
MagPure Circulating DNA Mini Kit is designed for purification of high quality circulating DNA (cfDNA) fromcell-free body fluids (such as plasma, serum). The purified DNA is suitable for direct use in downstream applications such as PCR, real-time PCR, biochip analysis and NGS.
Nucleic acid testing (NAT) is the method of choice for detection and quantification of a wide range of micro organisms. Primerdesign manufactures and supplies high quality quantitative real-time PCR kits for the detection and simultaneous quantification of numerous significant pathogens . A copy number standard curve is provided for quantification and an the internal extraction template (DNA or RNA), controls for the quality of the nucleic acid extraction and eliminates false negative results.
The kit is designed with the broadest possible detection profile to ensure that all clinically relevant strains and subtypes are detected. Target sequences are selected by working with data from key opinion leaders in the field. Multiple sequence alignments and unprecedented real-time PCR expertise in design and validation ensure the best possible kit.
Details of the target and priming specificity are included in the individual handbooks above.
Packaged, optimised and ready to use. Expect Better Data.
Document
Exceptional value for money
Rapid detection of all clinically relevant subtypes
Positive copy number standard curve for quantification
Highly specific detection profile
High priming efficiency
Broad dynamic detection range (>6 logs)
Sensitive to < 100 copies of target
Accurate controls to confirm findings