GeneAb™ BSEP

Overview

• Detection kits for E.coli O157:H7
• Available in TaqMan format for analysis

E. coli O157:H7 is a rod-shaped, gram negative bacterium. It is an enterohemorrhagic strain of the common E. coli bacterium and infection by the O157:H7 strain is commonly associated with hemorrhagic colitis. E. coli O157:H7 is recognized by its somatic (cell wall) antigen (O157) and its flagella antigen (H7). In addition, E. coli O157:H7 is known to produce Shiga-like toxins, which cause severe symptoms. While most patients can recover from the infection, up to 15% of the patients may develop hemolytic uremic syndrome, a type of kidney failure that could be fatal. Infection of E. coli O157:H7 usually results from consumption of poorly prepared food including undercooked meat (particularly ground beef), untreated water or raw unpasteurized milk.

E.coli O157:H7 TaqMan PCR Kit, 100 reactions

• Ready to use format, including Master Mix for the target and PCR control to monitor for PCR inhibition and validate the quality
• Specific Primer and Probe mix for the pathogen/virus/viroid of interest
• Primer and Probe mix
• Positive and negative control to confirm the integrity of the kit reagents

E.coli O157:H7 TaqMan PCR Probe/Primer Set and Controls, 100 reactions

• Specific Primer/Probe mix and Positive Control for the pathogen/virus/viroid of interest
• Nuclease-free water
• Can be used together with Norgen’s PCR Master Mix (#28007) or customer supplied master mix

For research use only and NOT intended for in vitro diagnostics.

Details

Supporting Data

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Storage Conditions and Product Stability
All kit components can be stored for 2 years after the date of production without showing any reduction in performance.

All kit components should be stored at -20°C upon arrival. Repeated thawing and freezing (> 2 x) of the Master Mix and Positive Control should be avoided, as this may affect the performance of the assay. If the reagents are to be used only intermittently, they should be frozen in aliquots.

Component	Cat. TM41350 (100 preps)	Cat. TM41310 (100 preps)
MDx TaqMan 2X PCR Master Mix	2 x 700 μL	–
E. coli O157:H7 Primer & Probe Mix	280 μL	280 μL
E. coli O157:H7 Positive Control	150 μL	150 μL
Nuclease-Free Water (Negative Control)	1.25 mL	1.25 mL
Product Insert	1	1

Description

Attogene’s Nano-clay Powder is comprised of thin layers and each layer has a thickness of one to a few nanometers length from a few hundred to several thousand nanometers. Nanparticles are utilized as fillers or additives in polymers for variety of desirable effects are receiving an increased interest for research and development. Different types of nanoparticles, such as nanocarbon, carbon nanotubes, nano-clays, and metal oxides, are recently employed to modify the polymer performance.

The viable interest is in the utilization of nano-clays for the alteration of polymeric material for numerous applications. This may be indicated from the increased commercial interest, and utilization of clay nanocomposites. It is an organic and hydrophilic. Moreover, the nanoclays are added in the polymers to increase the mechanical properties of polymers. In the nanocomposites, the permeability of the gases such as oxygen, carbon dioxide can be reduced by the addition of nanoclays. Nano clays have wide range of applications in food-packaging, pharmacy, used as a drug vehicle, textile industry etc.

Promotes flocculation of cyanobacteria cell debris, binds residual phosphate compounds and promotes gradual settling of the cyanobacterial biomass on the sediment.

Sample Package Size: 500g