12 x 8 strips (96 tests)

Cysticercosis is caused by larval cysts of the tapeworm Taenia solium and is a major cause of adult onset seizures in most developing countries. Diagnosis is based on imaging findings, but in many cases it is not conclusive for the diagnosis. Serological assays has an important place on the final diagnosis of the disease. The ELISA kit hs shown appropriate performances for the serological screening of cysticercosis.

Name of Product

Taenia solium – IgG ELISA

Catalog Number

AF 9700

Short Info

Cysticercosis is caused by larval cysts of the tapeworm Taenia solium and is a major cause of adult onset seizures in most developing countries. Diagnosis is based on imaging findings, but in many cases it is not conclusive for the diagnosis. Serological assays has an important place on the final diagnosis of the disease. The ELISA kit hs shown appropriate performances for the serological screening of cysticercosis.

This product is manufactured by Bordier Affinity Products in Switzerland and distributed in Germany exclusively by Milenia Biotec.

Method/Platform

ELISA in microplate format

Range/Assay Sensivity

98% sensitivity, 98% specificity

Test Principle

The kit provides all the material needed to perform 96 enzyme-linked immunosorbent assays (ELISA) on breakable microtitration wells sensitized with Taenia solium cyst soluble antigens. Specific antibodies in the sample will bind to these antigens and washing will remove unspecific antibodies. The presence of parasite specific antibodies is detected with a Protein A – alkaline phosphatase conjugate. A second washing step will remove unbound conjugate. Revealing bound antibodies is made by the addition of pNPP substrate which turns yellow in the presence of alkaline phosphatase. Color intensity is proportional to the amount of Taenia solium specific antibodies in the sample. Potassium phosphate is added to stop the reaction. Absorbance at 405 nm is read using an ELISA microplate reader. The test can be performed with automatic systems, but this must be validated by the user.

Cysticercosis is caused by larval cysts of the tapeworm Taenia solium and is a major cause of adult onset seizures in most developing countries. Diagnosis is based on imaging findings, but in many cases it is not conclusive for the diagnosis. Serological assays has an important place on the final diagnosis of the disease. The ELISA kit hs shown appropriate performances for the serological screening of cysticercosis.

Introduction

The HiPure Total RNA Kit provides fast purification of high-quality RNA from cells, tissues, and yeast using silica-membrane spin columns with a binding capacity of 100μg RNA. There is no need for phenol/chloroform extractions and time-consuming steps such as CsCl gradient ultracentrifugation or isopropanol precipitation. RNA purified using the HiPure Total RNA Purification System can be used for applications such as RT-PCR, Northern blotting, poly A+ RNA (mRNA) purification, nuclease protection, and in vitro translation.

Details

Specifications

Principle

HiPure RNA technology simplifies total RNA isolation. Samples are first lysed and then homogenized. Ethanol is added to the lysate to provide ideal binding conditions. The lysate is then loaded onto the HiPure silica membrane and RNA binds to the silica membrane, and all contaminants are efficiently washed away. For certain RNA applications that are sensitive to very small amounts of DNA, the residual amounts of DNA remaining can be removed using a convenient on-column DNase treatment. Pure, concentrated RNA is eluted in water.

Advantages

• Efficient removal of DNA – unique genomic DNA removal column without DNase treatment
• High quality – high purity total RNA can be directly used in various sensitive downstream applications
• Fast – several samples can be extracted in 20 minutes by column method
• Safe – no phenol chloroform extraction required
• Sensitive – RNA can be recovered at the level of PG

Kit Contents

Storage and Stability

The Kit can be stored dry at room temperature (15-25°C) and are stable for at least 18 months under these conditions.

The HiPure Total RNA Kit provides fast purification of high-quality RNA from cells, tissues, and yeast using silica-membrane spin columns with a binding capacity of 100μg RNA. There is no need for phenol/chloroform extractions and time-consuming steps such as CsCl gradient ultracentrifugation or isopropanol precipitation. RNA purified using the HiPure Total RNA Purification System can be used for applications such as RT-PCR, Northern blotting, poly A+ RNA (mRNA) purification, nuclease protection, and in vitro translation.

Introduction

1.Usages:
For fungal culture and sterile inspection of drugs and biological products.
 
Principle:
Peptone provide carbon and nitrogen sources; yeast extract powder provides B vitamins;
glucose provide energy; potassium hydrogen phosphate as buffer; magnesium sulfate to
provide essential trace elements.
 
Recipe (per liter):
Peptone 5.0g
Yeast extract powder 2.0g
Glucose 20.0g
Dipotassium hydrogen phosphate 1.0g
Magnesium sulfate 0.5g
Final pH 6.4 ± 0.2
 
Storage: Keep container tightly closed, store in a cool, dry place, away from bright light. Storage period of 3 years.

250g