
Description
Specifications
Clone | IHC531 |
Source | Mouse Monoclonal |
Positive Control | Tonsil |
Dilution Range | 1:200 |
Cluster of differentiation 2 (CD2) is a useful early T-cell lineage restricted antigen that is present in T-cell differentiation. As a pan-T-cell marker, CD2 staining is used for recognizing practically all normal T-cells, but may be deleted in some T-cell neoplasms. Since CD2 is present in most precursor and mature T-cell leukemias and lymphomas, it is useful in the evaluation of lymphoid malignancies. By using CD2 and CD25 staining, the recognition of systemic mastocytosis and mastocytic leukemia is supported.
Clone | IHC531 |
Source | Mouse Monoclonal |
Positive Control | Tonsil |
Dilution Range | 1:200 |
The Small RNA Library Prep Kit for Illumina consists of all the reagents and components required to generate small RNA libraries to be used for next-generation sequencing on an Illumina platform. All molecular reagents including adaptors, primers, enzyme mixes and buffers are provided. A purification module is also provided for rapid purification of nucleic acid products generated at various steps of the workflow. The purification module utilizes Norgen’s patent resin technology which enhances recovery of desired library intermediates or final products. The library prep workflow could be used for different forms of input including purified total RNA or enriched small RNA, as well as RNA from low content inputs such as plasma, serum and urine.
Workflow
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Kit Specifications | |
Number of preps | 24 |
Number of indices provided | 12 (for 24 preps, indexes 1-24 or 25-48) |
Time required to complete 6 libraries | As little as 5 hours |
Input RNA required | As little as 50 pg |
Storage Conditions and Product Stability
Some components require storage at -20°C, 4°C or room temperature. See individual components and box labels for storage conditions.
Step | Component | Cat. 64600 (24 preps) |
---|---|---|
3′ AdaptorLigation to Template RNA | 3′ Adaptor | 30 µL |
3′ Adaptor Ligation Master Mix | 320 µL | |
T4 RNA Ligase 2 (Truncated) | 35 µL | |
5′ Adaptor Ligation | 5′ Adaptor | 30 µL |
5′ Adaptor Ligation Master Mix | 320 µL | |
T4 RNA Ligase 1 | 35 µL | |
cDNA Synthesis from Ligated RNA Product | Reverse Primer | 30 µL |
cDNA Synthesis Master Mix | 220 µL | |
TruScript ReverseTranscriptase | 35 µL | |
PCR Amplification | 2x NGS PCR Master Mix | 1.32 µL |
PCR Reverse Primer | 81 µL | |
Forward Index Primer | Included in Small RNA Library Prep Forward Index Primers (# 64640 or # 64610) | |
Size Selection | NGS MW Ladder | 50 µL |
NGS Control Ladder | 50 µL | |
Loading Dye | 300 µL | |
Nuclease-Free Water | 1.25 µL |
Component | Cat. 63500 (75 preps) |
---|---|
Buffer RL | 40 mL |
Wash Solution A | 38 mL (Reconstituted to 128 mL) |
Elution Solution A | 6 mL |
Columns | 75 |
Gel Filtration Columns | 24 |
Collection Tubes | 75 |
Elution Tubes | 75 |
Product Insert | 1 |
Clone | IHC019 |
Source | Mouse Monoclonal |
Positive Control | Bladder, Colon Carcinoma, Colon, Thyroid Carcinoma |
Dilution Range | 1:200 |
Cytokeratin 19 (CK19) forms intermediate filaments found in the intracytoplasmic cytoskeleton of epithelial tissue and provides mechanical support. Anti-Cytokeratin 19 stains epithelia and epithelial malignancies such as carcinomas of the colon, stomach, pancreas, biliary tract, liver, and breast. Cytokeratin 19 is a useful marker for distinguishing hepatocellular carcinoma from intrahepatic cholangiocarcinoma. This differentiation is improved when stained in combination with Cytokeratin 7, CAM5.2l, Ber-EP4/MOC31, HepPar1 and TTF1. Cytokeratin 19 staining can also be used to recognize thyroid papillary carcinomas.
Mastitis is the single most costly disease of dairy cattle resulting in the reduction of milk yield and quality. The inflammation of the utter is mainly caused by bacteria, and Staphylococcus aureus (S. aureus) is often considered the most common cause of contagious mastitis in dairy herds. S. aureus infection is estimated to be present in up to 90% of dairy farms and is responsible for 35% of the economic loss in the dairy industry. S. aureus is a facultatively anaerobic, Gram positive bacterium. The majority of S. aureus strains are catalase-positive and coagulase-positive, which forms the basis of traditional identification methodology.
Staphylococcus aureus Quantified Bacterial DNA Standard is prepared from cultured bacteria using Norgen’s sample preparation technology. The purified DNA is quantified vigorously using multiple methods including spectrophotometry, gel densitometry and real-time PCR. It is intended to be used as a positive control or PCR quantification standard for Staphylococcus aureus.
Figure 1 / 1
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Volume Provided | 250 µL |
DNA Quantity | 2 x 104 copies per µL |
Storage Conditions
Upon receipt, store Norgen’s Staphylococcus aureus Quantified Bacterial DNA Standard at -20oC or lower. Avoid multiple freeze-thaw cycles. If needed, prepare smaller working aliquots and store at -20oC or lower.
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