Description
Specifications
Clone | IHC018 |
Source | Mouse Monoclonal |
Positive Control | Breast, Breast Carcinoma |
Dilution Range | 1:200 |
Cytokeratin 18 (CK18) is present in simple, glandular, and transitional epithelial cells, but is absent in stratified epithelial cells. CK18 usually multimerizes with Cytokeratin 8, and Anti-Cytokeratin 18 is useful for detecting adenocarcinomas of simple and glandular epithelium origin, as well as poorly differentiated squamous carcinoma cells.
Clone | IHC018 |
Source | Mouse Monoclonal |
Positive Control | Breast, Breast Carcinoma |
Dilution Range | 1:200 |
Usages:
For cultivation of bifidobacteria and lactic acid metabolites test.
Principle:
Peptone, yeast extract provide nitrogen, vitamins, and growth factors; glucose as
fermentable sugars; cysteine – HCl, vitamin K1, hemin, magnesium sulfate, calcium
chloride, sodium chloride, sodium bicarbonate training offers various bifidobacteria growth factor; dipotassium hydrogenphosphate, potassium dihydrogenphosphate buffer.
Formulation ( per liter):
Peptone 20g
Glucose 5g
Yeast powder 10g
Cysteine – hydrochloride 0.5g
Calcium chloride 0.008g
Magnesium sulfate 0.008g
Dipotassium hydrogen phosphate 0.04g
Potassium dihydrogen phosphate 0.04g
Sodium bicarbonate 0.4g
Sodium chloride 0.08g
Final pH 6.0 ± 0.2
Storage: Keep container tightly closed, store in a cool, dry place, away from bright light. Storage period of 3 years.
250g
Techinical parameter
Item No | SF7009 | SF9009 | SF7012 | SF9012 |
Ice condenser capacity | 9kg | 12kg | ||
Ice condenser temperature | -70 C° | 90℃ | -70 C° | -90℃ |
Cold trap volume | 15L | 20L | ||
Freeze dried chamber | High permeability organic glass/multi manifold external valve/stainless steel chamber | |||
Sample shelf | ●φ260mm/485mm.Single layer area 0.055m²/0.185m².3-10 layers/optional ● Electric heating/manual capping/optional | |||
Vacuum pump | Extreme vacuum 5*10*mbar with a pumping capacity of 8m³/h optional pump of various types | |||
Oil mist filter | Standard configuration | |||
Vacuum pump connection pipe | Single forming stainless steel flexible pipe DN16 ISO-KF L-1.2m | |||
Anti-corrosive treatment | Cold trap,condensing coil/all equipped with PTFE anti-corrosion treatment as standard. Freeze-drying organic solvents | |||
External valve | 1-48 ports/optiona | |||
Eutectic point judgment | Support/Optional | |||
Data export analysis | Support/Optional | |||
Power | 0.9kw | 1.5kw | 1kw | 1.6KW |
Size | 525*570*1080mm |
Model:
SF7009,SF9009,SF7012,SF9012
Brand:
Yingtai
The HiPure Total RNA Kit provides fast purification of high-quality RNA from cells, tissues, and yeast using silica-membrane spin columns with a binding capacity of 100μg RNA. There is no need for phenol/chloroform extractions and time-consuming steps such as CsCl gradient ultracentrifugation or isopropanol precipitation. RNA purified using the HiPure Total RNA Purification System can be used for applications such as RT-PCR, Northern blotting, poly A+ RNA (mRNA) purification, nuclease protection, and in vitro translation.
Specifications
Features | Specifications |
Main Functions | Isolation total RNA (not include miRNA) from animal tissues, cells and simple plant tissues using one column |
Applications | RT-PCR, qRT-PCR, Northern hybridization, second generation sequencing |
Purification method | Mini spin column |
Purification technology | Silica technology |
Process method | Manual (centrifugation or vacuum) |
Sample type | Animal soft tissue, cultured cells, lymphocytes, simple plant tissue |
Sample amount | Cells: ≤1 x 107 Animal tissue: 1-20 mgPlant leaves: 50-150 mg Yeast cells: 5 x 106 |
Yield | 2-100μg |
Elution volume | ≥50μl |
Time per run | ≤15 minutes(1-24 samples) |
Liquid carrying volume per column | 800µl |
Binding yield of column | 100µg |
HiPure RNA technology simplifies total RNA isolation. Samples are first lysed and then homogenized. Ethanol is added to the lysate to provide ideal binding conditions. The lysate is then loaded onto the HiPure silica membrane and RNA binds to the silica membrane, and all contaminants are efficiently washed away. For certain RNA applications that are sensitive to very small amounts of DNA, the residual amounts of DNA remaining can be removed using a convenient on-column DNase treatment. Pure, concentrated RNA is eluted in water.
Advantages
Kit Contents
Contents | R401102 | R401103 |
Purification Times | 50 Preps | 250 Preps |
HiPure RNA Mini Columns | 50 | 250 |
2ml Collection Tubes | 50 | 250 |
RTL Lysis Buffer | 50 ml | 200 ml |
RNA Binding Buffer | 15 ml | 75 ml |
Buffer RW1 | 50 ml | 200 ml |
Buffer RW2* | 20 ml | 2 x 50 ml |
RNase Free Water | 10 ml | 30 ml |
Storage and Stability
The Kit can be stored dry at room temperature (15-25°C) and are stable for at least 18 months under these conditions.
The HiPure Total RNA Kit provides fast purification of high-quality RNA from cells, tissues, and yeast using silica-membrane spin columns with a binding capacity of 100μg RNA. There is no need for phenol/chloroform extractions and time-consuming steps such as CsCl gradient ultracentrifugation or isopropanol precipitation. RNA purified using the HiPure Total RNA Purification System can be used for applications such as RT-PCR, Northern blotting, poly A+ RNA (mRNA) purification, nuclease protection, and in vitro translation.