GeneAb™ Cytokeratin 18

The Permagen Xle400 low elution post magnet plate was designed for use in automation applications where volumes as low as 5 µL are required. This product was designed with our already popular product (LE400 above) in mind. We have enhanced and taken this product to the next level adding automation features such as integrated cushion base and corner brackets to keep the microplate in the proper location

SBS SLAS Footprint (127.75mm x 85.50mm) to fit into any automated liquid handling robot

Features include solid aluminum alloy construction and hard coat anodized finish for years of trouble-free use, and compatible with any magnetic beads

SKU #

A072524

Features

• SBS/ SLAS footprint
• Solid Aluminum alloy design
• Precision manufactured for more consistent results
• Fast magnetic bead separations
• Integrated bracket to help keep microplate in place and flat
• Integrated cushion base plate for precise pipetting
• Made in USA 
• For Research use only

Compatibility

• Full- skirt PCR plates such as Bio-Rad® Hard-shell PCR (recommended)
• Semi- skirt PCR plates (manual use) 
• Non-skirted PCR plates (manual use)
• Any magnetic beads
• Any common liquid handlers i.e. Tecan®, Opentrons®, Hamilton®, Agilent®, Beckman®, etc.

Volumes

Maximum  – 150 µL

Minimum  – 5 µL 

The Permagen Xle400 low elution post magnet plate was designed for use in automation applications where volumes as low as 5 µL are required. This product was designed with our already popular product (LE400 above) in mind. We have enhanced and taken this product to the next level adding automation features such as integrated cushion base and corner brackets to keep the microplate in the proper location

Overview

• Removes primer dimers
• Simultaneously clean-up and normalize PCR products 
• Fast (less than 20 minutes), high-throughput and easy processing using centrifugation
• Sufficient elution volume (100 µL) for repeat or future assays 
• Non-magnetic bead purification 

Norgen’s NGS Normalization 96-Well Kit allows for high-throughput NGS library PCR amplicon clean-up and normalization of NGS library PCR product concentration. The clean-up removes all PCR by-products including primers, dimers, enzymes and unincorporated nucleotides during the library prep PCR step. Purification is based on 96-well column chromatography using Norgen’s proprietary resin as the separation matrix. The amount of Norgen’s matrix in each 96 well is optimized to have a limited binding capacity, thus each well can elute an equal concentration of PCR product. The process involves first adding 3 volumes of Buffer SK to the PCR product, and the mixture is then loaded onto the 96-Well Normalization Plate. The bound PCR amplicon is then washed with the provided Wash Solution A in order to remove any remaining impurities, and the purified PCR amplicon is eluted with Elution Buffer B. The purified and normalized library PCR amplicon can then be used in NGS workflows and other sequencing applications.

Details

Supporting Data

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Storage Conditions
All solutions should be kept tightly sealed and stored at room temperature. This kit is stable for 2 years after the date of shipment.

Component	Cat. 61900 (192 preps)
Buffer SK	30 mL
Wash Solution A	2 x 38 mL
Elution Buffer B	30 mL
96-Well Normalization Plate	2
96-Well Collection Plate	2
96-Well Elution Plate	2
Adhesive Tape	1
Product Insert	1

Overview

• Detection kits for Legionella sp.
• Lyophilized format designed for room temperature shipping
• Available in TaqMan format for analysis

Norgen’s Legionella sp. TaqMan PCR Lyophilized Kit is designed for the detection of Legionella sp. specific DNA in a real-time PCR based on the use of TaqMan® technology. The lyophilized format is designed to ship the kit at ambient temperature.

Norgen’s Legionella sp. TaqMan Lyophilized Probe/Primer and Control Set is designed for the detection of Legionella sp. specific DNA in a real-time PCR based on the use of TaqMan® technology. The lyophilized format is designed to ship the kit at ambient temperature.

Details

Supporting Data

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Storage Conditions and Product Stability

• All kit components should be stored at -20°C upon arrival.
• Once reconstituted, repeated thawing and freezing (>2 times) of the Master Mix and Positive Control should be avoided, as this may affect the performance of the assay. If the reagents are to be used only intermittently, they should be frozen in aliquots.
• All kit components can be stored for 2 years after the date of production without showing any reduction in performance.

Component	Cat. TM64450L (100 preps)	Cat. TM64410L (100 preps)
MDx TaqMan 2X PCR Master Mix (Lyo)	4 x 350 μL	–
Legionella sp. Primer & Probe Mix (Lyo)	280 μL	280 μL
Legionella sp. Positive Control (Lyo)	150 μL	150 μL
Nuclease-Free Water (Negative Control)	3 x 1.25 mL	1 x 1.25 mL
Product Insert	1	1