E-cadherin is an intercellular adhesion molecule present in epithelial cells. Anti-E-cadherin stains glandular epithelium, as well as lung, gastrointestinal and ovarian adenocarcinomas. A panel of antibodies against E-cadherin and p120 is also used to differentiate ductal (membranous staining) and lobular breast cancer (cytoplasmic staining). Anti-E-cadherin also stains some thyroid cancers.
Discover our Modified Tryptone Soya Broth, designed for the enrichment and cultivation of a wide range of microorganisms, ensuring accurate and reliable microbiological testing.
Discover our Modified Tryptone Soya Broth, designed for the enrichment and cultivation of a wide range of microorganisms, ensuring accurate and reliable microbiological testing.
HiPure DNA Clean Up 96 Kit uses proprietary chemistry and HiPure technology to recover DNA Fragments between 60bp-10kbp with yields exceeding 80%. DNA is suitable for ligations, PCR, sequencing, restriction digestion, or various labeling reactions. In addition, this kit can be also used to recover DNA directly from enzymatic reactions such as PCR and enzyme digestion reactions.
Specifications
| Features | Specifications |
| Main Functions | Recover DNA fragments between 60bp-10kbp from PCR, Reactions, crude DNA using 96 well Bind Plate |
| Applications | PCR, sequencing, labeling reactions, ligations and restriction digestion, etc. |
| Purification method | 96 well Bind Plate |
| Purification technology | Silica technology |
| Process method | Manual (centrifugation or vacuum) |
| Sample type | PCR product, enzymatic reaction |
| Sample amount | Appropriate |
| Recovery | ≥80% |
| Elution volume | ≥75μl |
| Time per run | ≤60 minutes |
| Liquid carrying volume per column | 800µl |
| Binding yield of column | 20µg |
The HiPure system uses a simple bind-wash-elute procedure. Gel slices are dissolved in a buffer containing a pH indicator, allowing easy determination of the optimal pH for DNA binding, and the mixture is applied to the column. Nucleic acids adsorb to the silica-gel membrane in the high-salt conditions provided by the buffer. Impurities are washed away and pure DNA is eluted with a small volume of low-salt buffer provided or water, ready to use in subsequent applications.
Advantages
Kit Contents
| Contents | D212201 | D212202 | D212203 |
| Purification Times | 1 x 96 Preps | 4 x 96 Preps | 20 x 96 Preps |
| Buffer GDP | 120 ml | 400 ml | 4 x 400 ml |
| Buffer DW2 | 20 ml | 100 ml | 3 x 100 ml |
| Elution Buffer | 20 ml | 20 ml | 30 ml |
| HiPure DNA Plate | 1 | 4 | 20 |
| 2.2ml Collection Plate | 1 | 4 | 20 |
| 1.6 ml Collection Plate | 1 | 4 | 20 |
| 0.8 ml Collection Plate | 1 | 4 | 20 |
Storage and Stability
The Kit can be stored dry at room temperature (15-25°C) and are stable for at least 18 months under these conditions. If any precipitates form in the buffers, warm at 37℃ to dissolve.
HiPure DNA Clean Up 96 Kit uses proprietary chemistry and HiPure technology to recover DNA Fragments between 60bp-10kbp with yields exceeding 80%. DNA is suitable for ligations, PCR, sequencing, restriction digestion, or various labeling reactions. In addition, this kit can be also used to recover DNA directly from enzymatic reactions such as PCR and enzyme digestion reactions.
DADPS (dialkoxydiphenylsilane) Biotin Alkyne probes eliminate a major limitation of the streptavidin-biotin affinity purification. This reagent contains a biotin moiety and an azide reactive moiety. DADPS probe can be used in biomolecular labeling and proteomic studies. Reagent grade, for research purpose. Please contact us for GMP-grade inquiries.
DADPS (dialkoxydiphenylsilane) Biotin Alkyne probes eliminate a major limitation of the streptavidin-biotin affinity purification. This reagent contains a biotin moiety and an azide reactive moiety. DADPS probe can be used in biomolecular labeling and proteomic studies. Reagent grade, for research purpose. Please contact us for GMP-grade inquiries.
