
Description
Specifications
Clone | IHC564 |
Source | Mouse Monoclonal |
Positive Control | Breast |
Dilution Range | 1:200 |
E-cadherin is an intercellular adhesion molecule present in epithelial cells. Anti-E-cadherin stains glandular epithelium, as well as lung, gastrointestinal and ovarian adenocarcinomas. A panel of antibodies against E-cadherin and p120 is also used to differentiate ductal (membranous staining) and lobular breast cancer (cytoplasmic staining). Anti-E-cadherin also stains some thyroid cancers.
Clone | IHC564 |
Source | Mouse Monoclonal |
Positive Control | Breast |
Dilution Range | 1:200 |
The HiPure Plasmid DNA Plus 96 Kit enables up to 96 minipreps to be performed simultaneously in less than 45 minutes on the Vacuum Manifold (Qiavac 96). This kit provides a fast, simple,and cost-effective plasmid DNA high-throughput method for routine molecular biology laboratory applications. HiPure Kits use silica membrane technology to eliminate the cumbersome steps associated with loose resins or slurries.Plasmid DNA purified with Kits is immediately ready for use. Phenol extraction and ethanol precipitation are not required, and high quality plasmid DNA is eluted in a small volume of Tris buffer or water.
Specifications
Features | Specifications |
Main Functions | Isolation up to 20µg plasmid DNA from 1.5ml bacterial culture using 96 well bind plate and 96 filterplate |
Applications | Enzyme digestion, sequencing, PCR, labeling, etc. |
Purification method | 96 well plate |
Purification technology | Silica technology |
Process method | Manual (centrifugation or vacuum) |
Sample type | Conventional plasmid, plasmid less than 30KB |
Sample amount | 1-1.5ml(x96) |
Yield | 1-15µg/1ml |
Elution volume | ≥70μl |
Time per run | ≤60 minutes |
Liquid carrying volume per column | 800µl |
Binding yield of column | 70µg |
The HiPure Plasmid procedure is based on alkaline lysis of bacterial cells followed by adsorption of DNA onto silica in the presence of high salt. The unique silica membrane used in the kit completely replaces glass or silica slurries for plasmid DNA minipreps. The procedure consists of 3 basic steps: Preparation and clearing of a bacterial lysate by alkaline method,then transfer the supernatant to column to bind DNA. After washing proteins and other impurities, nucleic acid was finally eluted with low-salt buffer (10mm Tris, pH9.0, 0.5mm EDTA).
Advantages
Kit Contents
Contents | P100601 | P100602 | P100603 |
Purification Times | 1 x 96 Preps | 4 x 96 Preps | 20 x 96 Preps |
RNase A | 5 mg | 20 mg | 100 mg |
Buffer P1 | 30 ml | 120 ml | 600 ml |
Buffer P2 | 30 ml | 120 ml | 600 ml |
Buffer P3 | 40 ml | 180 ml | 800 ml |
Buffer PW1 | 100 ml | 500 ml | 2 x 1000 ml |
Buffer PW2 | 50 ml | 2 x 100 ml | 4 x 200 ml |
Elution Buffer | 150 ml | 60 ml | 300 ml |
Lysate Clear Plate | 1 | 4 | 20 |
HiPure DNA Plate | 1 | 4 | 20 |
1.6 ml Collection Plate | 1 | 4 | 20 |
0.5ml Elute Plate | 1 | 4 | 20 |
Storage and Stability
The kit components can be stored dry at room temperature (15-25°C) and are stable for at least 18 months under these conditions. If any precipitates form in the buffers, warm at 37℃ to dissolve. After addition of RNase A,Buffer P1 is stable for 6 months when stored at 2-8°C.
For any technical problems or customized products, please contact us.
F&Q about Endotoxin-free Plasmid Extraction Kit — P1156 ←click here
The HiPure Plasmid DNA Plus 96 Kit enables up to 96 minipreps to be performed simultaneously in less than 45 minutes on the Vacuum Manifold (Qiavac 96). This kit provides a fast, simple,and cost-effective plasmid DNA high-throughput method for routine molecular biology laboratory applications. HiPure Kits use silica membrane technology to eliminate the cumbersome steps associated with loose resins or slurries.Plasmid DNA purified with Kits is immediately ready for use. Phenol extraction and ethanol precipitation are not required, and high quality plasmid DNA is eluted in a small volume of Tris buffer or water.
Nucleic acid testing (NAT) is the method of choice for detection and quantification of a wide range of micro organisms. Primerdesign manufactures and supplies high quality quantitative real-time PCR kits for the detection and simultaneous quantification of numerous significant pathogens . A copy number standard curve is provided for quantification and an the internal extraction template (DNA or RNA), controls for the quality of the nucleic acid extraction and eliminates false negative results.
The kit is designed with the broadest possible detection profile to ensure that all clinically relevant strains and subtypes are detected. Target sequences are selected by working with data from key opinion leaders in the field. Multiple sequence alignments and unprecedented real-time PCR expertise in design and validation ensure the best possible kit.
Details of the target and priming specificity are included in the individual handbooks above.
Packaged, optimised and ready to use. Expect Better Data.
Exceptional value for money
Rapid detection of all clinically relevant subtypes
Positive copy number standard curve for quantification
Highly specific detection profile
High priming efficiency
Broad dynamic detection range (>6 logs)
Sensitive to < 100 copies of target
Accurate controls to confirm findings
endo-BCN-PEG2-Acrylate is a PEG linker containing a BCN group and an acrylate group. The BCN group enables copper free click chemitry with azide-tagged molecules. The acrylate group enables Michael addition reactions. The hydrophilic PEG spacer increases solubility in aqueous media. Reagent grade, for research use only.
endo-BCN-PEG2-Acrylate is a PEG linker containing a BCN group and an acrylate group. The BCN group enables copper free click chemitry with azide-tagged molecules. The acrylate group enables Michael addition reactions. The hydrophilic PEG spacer increases solubility in aqueous media. Reagent grade, for research use only.
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