
Description
Specifications
Clone | IHC002 |
Source | Mouse Monoclonal |
Positive Control | Breast Carcinoma |
Dilution Range | 1:200 |
The Her2/Neu (c-erbB-2) proto-oncogene is a transmembrane receptor tyrosine kinase that is clinically indicated in a number of carcinomas. Overexpression of the c-erbB-2 protein has been associated with ductal breast cancer, as well as pulmonary and gastric adenocarcinomas. A correlation between Her2 and p53 has also been documented, as overexpression of both proteins has been associated with early invasion and metastasis in bladder cancer.
Clone | IHC002 |
Source | Mouse Monoclonal |
Positive Control | Breast Carcinoma |
Dilution Range | 1:200 |
Herpes Simplex Virus 1 (HSV-1) is a member of the herpes virus family, Herpesviridae. HSV-1 has a relatively large double-stranded DNA genome. HSVs are primarily transmitted by sexual intercourse, direct contact with lesions or perinatally. Most HSV positive cases are characterised by lesions on the skins and mucous membranes of the mouth and genitals. HSV infection can be either primary or a recurrence of a previous infection. More than 90% of the primary HSV infections are asymptomatic. Primary infection with HSV-1 can lead to gingivostomatitis, eczema herpeticum, keratoconjunctivitis and encephalitis. The primary symptoms of a secondary infection are skin lesions in the nose, mouth and genital regions. The infection is contagious, mainly during an epidemic.
HSV-1 TaqMan PCR Kit, 100 reactions
HSV-1 TaqMan PCR Probe/Primer Set and Controls, 100 reactions
For research use only and NOT intended for in vitro diagnostics.
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Storage Conditions and Product Stability
All kit components can be stored for 2 years after the date of production without showing any reduction in performance.
All kit components should be stored at -20°C upon arrival. Repeated thawing and freezing (> 2 x) of the Master Mix and Positive Control should be avoided, as this may affect the performance of the assay. If the reagents are to be used only intermittently, they should be frozen in aliquots.
Component | Cat. TM32650 (100 preps) | Cat. TM32610 (100 preps) |
---|---|---|
MDx TaqMan 2X PCR Master Mix | 2 x 700 μL | – |
HSV-1 Primer & Probe Mix | 280 μL | 280 μL |
HSV-1 Positive Control | 150 μL | 150 μL |
Nuclease-Free Water (Negative Control) | 1.25 mL | 1.25 mL |
Product Insert | 1 | 1 |
Opentrons Tough 0.2 mL 96-Well PCR Plate, Full Skirt, RNase/DNase-free. Strong polycarbonate frame with thin-walled polypropylene wells for high-performance thermal cycling without warping. Ideal for use within automated liquid handlers with or without a gripper. 0.2 mL total well volume, 0.1 mL working volume. 25 count. Not for therapeutic use SP-2396.
Opentrons Tough 0.2 mL 96-Well PCR Plate, Full Skirt, RNase/DNase-free. Strong polycarbonate frame with thin-walled polypropylene wells for high-performance thermal cycling without warping. Ideal for use within automated liquid handlers with or without a gripper. 0.2 mL total well volume, 0.1 mL working volume. 25 count. Not for therapeutic use SP-2396.
This kit is designed for the rapid preparation of genomic DNA from various tissue samples, cultured cells, viruses, bodily fluids and swabs using a rapid spin column protocol. Purified DNA is of an excellent yield and quality, and is immediately ready for any downstream application including PCR, qPCR, genotyping, sequencing and more. The protocol can be completed in approximately 80 minutes (including incubation time).
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Kit Specifications | |
Column Binding Capacity | 25 µg |
Average Yield:* HeLa Cells (1 x 106) Tissue (from 10 mg kidney) | 8 µg 10 µg |
Maximum Amount of Starting Material: Animal Tissues Cultured Cells Bodily Fluids (blood, saliva) Viral Suspension | 20 mg 3 x 106 cells 150 µL 150 µL |
Time to Complete 10 Purifications | 80 minutes |
* Yield will vary depending on the type of sample processed
Storage Conditions and Product Stability
The Proteinase K should be stored at -20°C upon arrival and after reconstitution. All other solutions should be kept tightly sealed and stored at room temperature. This kit is stable for 2 years after the date of shipment.
Component | Cat. 24700 (50 preps) | Cat. 24750 (100 preps) | Cat. 24770 (250 preps) |
---|---|---|---|
Digestion Buffer A | 25 mL | 2 x 25 mL | 5 x 25 mL |
Buffer SK | 30 mL | 2 x 30 mL | 5 x 30 mL |
Wash Solution A | 18 mL | 2 x 18 mL | 5 x 18 mL |
Elution Buffer B | 30 mL | 2 x 30 mL | 5 x 30 mL |
Proteinase K | 12 mg | 2 x 12 mg | 5 x 12 mg |
Spin Columns | 50 | 100 | 250 |
Collection Tubes | 50 | 100 | 250 |
Elution Tubes (1.7 mL) | 50 | 100 | 250 |
Product Insert | 1 | 1 | 1 |
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