GeneAb™ Kappa
The contamination of pathogenic microorganisms and their toxins in food and water is a serious issue for human health and safety. For instance, enterotoxins (SEs) produced by Staphylococcus aureus (S. aureus) are heat-stable, meaning pathological activity remains even after exposure to sterilization techniques and digestive proteases. Among the SEs, staphylococcal enterotoxin A (SEA) and B (SEB) are confirmed toxins which cause enteritis and food poisoning. Symptoms include nausea, vomiting, diarrhea, which in
severe cases, may lead to fatalities in children and the elderly. These SEs, known as superantigens, non-specifically activate Tcells, leading to proliferation which ultimately results in T-cell elimination. This activation directly and indirectly induces a massive release of inflammatory cytokines.
In addition to acute poisoning, researchers reported that these toxins may play roles in the pathogenesis of autoimmune diseases. More specifically, intestinal dysbiosis (enteromicrobial imbalance) was found in patients with rheumatoid arthritis (RA) and which may overwhelm the host immune defense functions by chronic exposure to excess amounts of these pathogens (7, 8). In animal models, SEs synergistically play a role in the pathogenesis of autoimmune-related diseases, such as atopic dermatitis, food allergies, colitis, arthritis, and systemic lupus erythematosus.
Attogene is offering this highly robust test to detect SEB by lateral flow in diverse liquid sample types.
Rapid dipstick test for detecting Staphylococcus Enterotoxin B (SEB) in liquid samples
Detects 10 ppb and greater Staphylococcus Enterotoxin B (SEB) in the sample
Name of Product
Hop Resistance Gene-Screen
Catalog Number
MGScHOR 1
Short Info
This test discovers hop resistance genes in a timely and precise manner
Method/Platform
PCR
Range/Assay Sensivity
10^4 – 10^5 cfu/mL
Test Principle
The technological basis for the GenLine tests is the polymerase chain reaction (PCR) combined with lateral flow Tests.
Labelled specific primers are used to amplify specific DNA fragments. In addition to the target gene, a control gene, which is also present in the PCR mixes, is amplified in order to make sure that the PCR process works properly.
The resulting PCR products carry the labels of the incorporated primers.
In a second part of the test, the created PCR products are detected by a lateral flow Test Strip. A “molecular sandwich” is formed and becomes visible as a line on the test Strip.
Brief Instructions
Storage
-15 to -20°C
Components
PCR-Polymerase Master-Mix, PCR-Primermix, Positive Kit-Control, PCR-Water
Name of Product
Hop Resistance Gene-Screen
Catalog Number
MGScHOR 1
Short Info
This test discovers hop resistance genes in a timely and precise manner
Method/Platform
PCR
Range/Assay Sensivity
10^4 – 10^5 cfu/mL
Test Principle
The technological basis for the GenLine tests is the polymerase chain reaction (PCR) combined with lateral flow Tests.
HiPure Gel Pure DNA Kit uses proprietary chemistry and HiPure technology to recover DNA fragments between 80bp-15kbp with yields exceeding 80%. DNA is suitable for ligations, PCR, sequencing, restriction digestion, or various labeling reactions. In addition, this kit can be also used to recover DNA directly from enzymatic reactions such as PCR and enzyme digestion reactions.
Note: This kit can only recover 200mg of gel at most. If it is larger than 200mg, the column may be blocked. This column can only recover 5μg of DNA at most.
Specifications
| Features | Specifications |
| Main Functions | Recover DNA fragments >100bp from agarose gel(<0.2g), purification of DNA from PCR or enzymatic reaction solution solution |
| Applications | PCR, NGS, labeling, ligation and enzyme digestion, etc. |
| Purification method | Micro spin column |
| Purification technology | Silica technology |
| Process method | Manual (centrifugation or vacuum) |
| Sample type | Agarose gel, PCR products, enzyme products |
| Sample amount | Agarose gel: ≤200mgDNA products: ≤300µl (≤5µg) |
| Recovery | 100-5000bp：85%, 5-10kb：60-70% |
| Elution volume | ≥10μl |
| Time per run | ≤30 minutes（1-24 samples） |
| Liquid carrying volume per column | 800µl |
| Binding yield of column | 20µg |
The HiPure system uses a simple bind-wash-elute procedure. Gel slices are dissolved in a buffer containing a pH indicator, allowing easy determination of the optimal pH for DNA binding, and the mixture is applied to the column. Nucleic acids adsorb to the silica-gel membrane in the high-salt conditions provided by the buffer. Impurities are washed away and pure DNA is eluted with a small volume of low-salt buffer provided or water, ready to use in subsequent applications.
Advantages
Kit Contents
| Contents | D211002 | D211003 |
| Purification Times | 100 Preps | 250 Preps |
| Buffer GDP | 60 ml | 125 ml |
| Buffer DW1 | 40 ml | 90 ml |
| Buffer DW2 | 20 ml | 50 ml |
| Elution Buffer | 20 ml | 30 ml |
| HiPure DNA Micro Columns | 100 | 250 |
| 2 ml Collection Tubes | 100 | 250 |
Storage and Stability
The Kit should be stored dry at room temperature (15-25°C) and are stable for at least 18 months under these conditions. If any precipitates form in the buffers, warm at 37℃ to dissolve
HiPure Gel Pure DNA Kit uses proprietary chemistry and HiPure technology to recover DNA fragments between 80bp-15kbp with yields exceeding 80%. DNA is suitable for ligations, PCR, sequencing, restriction digestion, or various labeling reactions. In addition, this kit can be also used to recover DNA directly from enzymatic reactions such as PCR and enzyme digestion reactions.
Note: This kit can only recover 200mg of gel at most. If it is larger than 200mg, the column may be blocked. This column can only recover 5μg of DNA at most.
