
Description
Specifications
Clone | IHC610 |
Source | Mouse Monoclonal |
Positive Control | Tonsil |
Dilution Range | 1:200 |
GeneAb™ Kappa
Clone | IHC610 |
Source | Mouse Monoclonal |
Positive Control | Tonsil |
Dilution Range | 1:200 |
For selective enrichment culture of Listeria monocytogenes in food.
Tryptone, proteose peptone, meat extract, yeast paste powder provide nitrogen sources, vitamins, amino acids, and growth factors; sodium chloride can maintain a balanced osmotic pressure; sodium dihydrogen phosphate and potassium dihydrogen phosphate as buffering agents; esculin are fermentable sugars; lithium chloride and other antibiotics can inhibit Gram negative bacteria and most Gram positive bacteria.
Ingredients | /liter |
Enzymatic digest of animal tissues ( Proteose peptone) | 5.0g |
Enzymatic digest of casein ( Tryptone) | 5.0g |
Meat extract | 5.0g |
Yeast extract | 5.0g |
Sodium chloride | 20.0g |
Disodium hydrogen phosphate dihydrate | 12.0g |
Potassium dihydrogen phosphate | 1.35g |
Aesculin | 1.0g |
Lithium chloride | 3.0g |
pH7.2±0.2 at 25°C |
Suspend 57.4g in 1 L of distilled or deionized water. Heat with frequent agitation and boil to completely dissolve the powder. Distribute into flasks. Autoclave at 121℃ for 15 minutes. Cool to 45-50℃,aseptically add the contents of 1 vial of Fraser Selective Supplement (SR0120) to each 225 mL of base to prepare FB1 solution; Add one reagent (SR0130) to each100 mL of FB2 culture medium to prepare FB2 enrichment solution.
Cultural characteristics observed after incubation at 35-37°C for 46~50 hours
Quality control strains | Growth | Characteristics |
Listeria monocytogenes ATCC19115 + Escherichia coli ATCC25922 + Enterococcus faecalis ATCC29212 | >20 cfu On PALCAM | Gray to black colony count with black halo |
Escherichia coli ATCC25922 | < 100 colonies on TSA | Inhibition |
Enterococcus faecalis ATCC29212 |
2-30℃,Keep container tightly closed, avoid direct sunlight.
Use before expiry date on the label.
1. When weighing the dehydrated medium, please wear masks to avoid causing respiratory system discomfort
2. Keep container tightly closed after using to prevent clumping.
Microbiological contamination was disposed by autoclaving at 121°C for 30 minutes.
On June 14, 2024
ISO 11290
Intended Use For selective enrichment culture of Listeria monocytogenes in food. Principle and Interpretation Tryptone, proteose peptone, meat extract, yeast paste powder provide nitrogen ……
Campylobacter jejuni is a curved, rod-shaped and microaerophillic gram negative bacterium. It is one of the most common causal agents of gastroenteritis with diarrhea as the main symptom. While infection of C. jejuni is seldom life-threatening, it is considered one of the most common food-borne bacteria with over 2 million people infected per year in US alone. Infection of C. jejuni usually results from consumption of poorly prepared food including undercooked meat (particularly poultry), untreated water or raw unpasteurized milk. Traditional identification of C. jejuni involves culturing, however the microaerophilic characteristic of this bacterium make the enrichment process laborious and costly.
Campylobacter jejuni TaqMan PCR Kit, 100 reactions
Campylobacter jejuni TaqMan PCR Probe/Primer Set and Controls, 100 reactions
For research use only and NOT intended for in vitro diagnostics.
Figure 1 / 3
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Storage Conditions and Product Stability
All kit components can be stored for 2 years after the date of production without showing any reduction in performance.
All kit components should be stored at -20°C upon arrival.
Component | Cat. TM36150 (100 preps) | Cat. TM36110 (100 preps) |
---|---|---|
MDx TaqMan 2X PCR Master Mix | 2 x 700 μL | – |
Campylobacter jejuni Primer & Probe Mix | 280 μL | 280 μL |
Campylobacter jejuni Positive Control | 150 μL | 150 μL |
Nuclease-Free Water (Negative Control) | 1.25 mL | 1.25 mL |
Product Insert | 1 | 1 |
Attogene’s Cadmium Lateral Flow Kit can be used to detect Cadmium in water samples at 5 ppb in the field.
Format: 10 test cassettes
Run Time: 15-30 minutes
Cadmium contamination is a serious worldwide environmental problem. As it is difficult to detoxify by chemical or biological methods, gradual Cadmium accumulation in the nervous and cardiovascular systems of the human body can subsequently cause serious diseases. Long-term health consequences of drinking cadmium-contaminated food and water include numerous negative health effects. Attogene’s Cadmium Lateral Flow test gives results conforming of 5ppb or greater. Using the supplied pipette, simply fill the vial with your water sample, place the water into the sample port and wait 15-30 minutes.
Format: 10 test cassettes
Run Time: 15-30 minutes
Detection: Cadmium in water samples at 5 ppb
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