GeneAb™ Nanog

Overview

• Ensure optimal results for sensitive applications like NGS
• Up to a tenfold increase in microRNA mapping during sequencing runs to reduce costs
• The purified exosomal RNA is free from any circulating RNA-binding proteins.
• No phenol extractions, Proteinase K treatment, nor carrier RNA.
• No time-consuming ultracentrifugation, filtration nor special syringes are required.
• Concentrate isolated RNA into a flexible elution volume ranging from 50 μL to 100 μL.
• Purification is based on EXTRAClean spin column chromatography that uses Norgen’s proprietary separation matrix.

Norgen’s EXTRAClean Plasma/Serum Exosome and Free-Circulating RNA Isolation Kit constitutes an all-in-one system for the purification of exosomes and the sequential isolation of RNA and free-circulating RNA from different plasma/serum sample volumes ranging from 50 μL and up to 10 mL. The purification is based on spin column chromatography that employs Norgen’s proprietary resin. The EXTRAClean columns undergo stringent processing and rigorous quality control measures to minimize contamination traces, ensuring optimal results for sensitive applications such as NGS. The kit is designed to isolate all sizes of RNA, including microRNA. The kit provides a clear advantage over other available kits in that they do not require any special instrumentation, protein precipitation reagents, extension tubes, phenol/chloroform or any protease treatments. Moreover, the kit allows the user to elute into a flexible elution volume ranging from 50 μL to 100 μL. The RNA isolated from the purified exosomes is free from any protein-bound circulating RNA and is of the highest integrity. Moreover, the free-circulating, protein-bound, RNA is free from any exosomal RNA. The purified RNA can be used in a number of downstream applications including real time PCR, NGS application, reverse transcription PCR, Northern blotting, RNase protection and primer extension, and expression array assays.

Details

Supporting Data

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Kit Specifications (Spin Column)
Minimum Plasma Input	4 mL
Maximum Plasma Input	10 mL
Size of RNA Purified	All sizes, including miRNA and small RNA (< 200 nt)
Elution Volume	50-100 μL
Time to Complete 10 Purifications	40-45 minutes
Average Yields	Variable depending on specimen

Storage Conditions and Product Stability
All solutions should be kept tightly sealed and stored at room temperature. This kit is stable for 2 years after the date of shipment. It is recommended to warm Lysis Buffer A for 20 minutes at 60°C if any salt precipitation is observed.

Ideal magnet for all of your low volume needs. From Low elution 96-well PCR applications to 384-well, the X396 has  you covered. No need to purchase two separate plates anymore. 96-well volumes as low as 5 µL are achieved from 96-well, PCR plates on the inside of the magnet, while the outside of each magnet will pull beads to well sides in 384-well plates.

ANSI/ SBS Footprint (127.75mm x 85.50mm) to fit into any automated liquid handling robot on bottom. ANSI/SBS footprint on top to accept all common microplates.

Integrated Cushion base for maximum recovery. Helps aid in set-up, robot positioning inconsistencies, and labware consumable inconsistencies

Features include solid aluminum alloy construction and hard coat anodized finish for years of trouble-free use, and compatible with any magnetic beads

SKU #

A040523

Features

• ANSI/ SBS footprint
• Compatible with 384-well and 96-well plates
• Solid Aluminum alloy design
• Precision manufactured for more consistent results
• Fast magnetic bead separations
• Made in USA 
• For Research use only

Compatibility

• 384-well pcr plates
• Some 384-well deep well plates
• Full-, Semi-, and un-Skirted 96-well PCR Plates
• Any magnetic beads
• Any common liquid handlers i.e. Tecan®, Opentrons®, Hamilton®, Agilent®, Beckman®, etc.

Volumes

384-Well PCR

Maximum – 39 µL 

Minimum – 5 µL 

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96-Well PCR

Maximum – 60 µL (0.2 mL for some beads)

Minimum – 4 µL

Ideal magnet for all of your low volume needs. From Low elution 96-well PCR applications to 384-well, the X396 has you covered. No need to purchase two separate plates anymore. 96-well volumes as low as 5 µL are achieved from 96-well, PCR plates on the inside of the magnet, while the outside of each magnet will pull beads to well sides in 384-well plates.