Introduction

MagZol Reagent is a reagent system for the isolation of total RNA from cells and tissues. The reagent, a single-phase solution consisting of phenol and guanidine isothiocyanate, is modification of the single-step RNA isolation method developed by Chomczynski and Sacchi. The sample is homogenized and lysed in MagZol Reagent which maintains the integrity of the RNA, while disrupting and denaturing endogenous RNases and other cellular components. Extraction of the lysate with chloroform further denatures proteins and separates the mixture into an organic and an aqueous phase. RNA remains exclusively in the aqueous phase, and is subsequently recovered by isopropanol.

This method is suitable for small quantities of tissue (<100mg) and cells (<5 X10⁶), and large quantities of tissue (up to 1g) and cells (<10⁸), of human, animal, plant, or bacterial origin. The simplicity of the MagZol Reagent method allows simultaneous processing of a large number of samples. The entire procedure can be completed in one hour. Total RNA prepared in this manner can be used for Northern blot analysis, dot blot hybridization, poly(A) + selection, in vitro translation, RNase protection assay, and molecular cloning. For use in amplification by thermal cycling, treatment of the isolated RNA with RNase-free DNase I is recommended when the two amplimers lie within a single exon.

Details

Specifications

Features	Specifications
Main Functions	Extract RNA from liquid samples by salting out method
Applications	RT-PCR, Northern hybridization, poly (a) enrichment, etc.
Purification technology	Acid phenol guanidine isothiocyanate
Process method	Manual (centrifugation)
Sample type	Various liquid samples
Sample amount	Flexible
Elution volume	Variation with sample size
Time per run	Variation with sample size

Advantages

• Flexible – sample amount can be adjusted according to the demand
• Cost performance -the most economical nucleic acid extraction technology

Storage and Stability

MagZol Reagent should be stored at 2-8°C upon arrival and is stable for at least 24 months under the condition. However, short-term storage (up to 12 weeks) at room temperature (15-25°C) does not affect its performance.

MagZol Reagent is a reagent system for the isolation of total RNA from cells and tissues. The reagent, a single-phase solution consisting of phenol and guanidine isothiocyanate, is modification of the single-step RNA isolation method developed by Chomczynski and Sacchi. The sample is homogenized and lysed in MagZol Reagent which maintains the integrity of the RNA, while disrupting and denaturing endogenous RNases and other cellular components. Extraction of the lysate with chloroform further denatures proteins and separates the mixture into an organic and an aqueous phase. RNA remains exclusively in the aqueous phase, and is subsequently recovered by isopropanol.

Introduction

MagPure A3 utilizes Magen’s solid-phase paramagnetic bead technology for high-throughput purification of PCR amplicons. AmPure utilizes an optimized buffer to selectively bind PCR amplicons 100bp and larger to paramagnetic beads. Excess primes, nucleotides, salts and enzymes can be removed using a simple washing procedure. The resulting purified PCR product is essentially free of contaminants.

Details

Specifications

Features	Specifications
Main Functions	Selectively recover DNA from PCR products and enzymatic reaction solution (Replace Beckmen or agencourt AmPure)
Applications	Sequencing, gene chip and qPCR, etc.
Purification technology	Magnetic beads technology
Process method	Manual (centrifugation or vacuum)
Sample type	PCR products, enzymatic reaction solution
Sample amount	Appropriate
Elution volume	≥15μl
Operation time	≤50 minutes

Principle

This product is based on the purification method of high binding magnetic particles. PCR amplicons mix with MagPure A3, 100bp and larger DNA binds to magnetic beads. Excessprimes, nucleotides, salts and enzymes can be removed using a simple washing procedure and finally DNA was eluted by Elution Buffer or Water.

Advantages

• Wide application – DNA products, enzyme digestion system, PCR reaction
• High recovery – suitable for 100bp-30kb fragments
• High purity – effectively remove dNTP, primer, primer dimer, salt ion and other impurities
• Strong applicability – manual operation or automatic workstation

Kit Contents

Contents	BXP-5	BXP-50	BXP-500
MagPure A3	5 ml	50 ml	500 ml

Storage and Stability

MagPure A3 should be stored at 2-8°C upon arrival and is stable up to 18 months under the condition. However, short-term storage (up to 12 weeks) at room temperature (15-25°C) does not affect its performance. Shake the reagent well before use. It should appear homogenous and consistent in color.DO NOT FREEZE.

MagPure A3 utilizes Magen’s solid-phase paramagnetic bead technology for high-throughput purification of PCR amplicons. AmPure utilizes an optimized buffer to selectively bind PCR amplicons 100bp and larger to paramagnetic beads. Excess primes, nucleotides, salts and enzymes can be removed using a simple washing procedure. The resulting purified PCR product is essentially free of contaminants.

Description

Biotin is extensively used in combination with streptavidin in biological assays.  Our biotin detection kits have multiple applications in detecting biotin attachment and efficiency of conjugation during biotinylation processes.  These kits are perfect for understanding the efficiency of the reaction and free biotin in your solutions that can interfear with Streptavidin-biotin interactions.

Attogene offers three kits to detect and quantify biotin using three different technologies including Lateral Flow, ELISA and Chemical.  these kits are accurate and sensitive compared with common instrumental analysis.

Can be used to calculate the amount of biotin in liquid samples including biological samples including biotinylated antibody, free biotin in solution, foods, plasma and serum as needed.  Also, can be used to quantify the % biotinylation following standard biotinylation reactions.

EL2021_EL: ELISA Biotinylation Quantification Kit

EL2021_EZ:  TNBSA Biotinylation Quantification Kit

EL2021_LF: Lateral Flow kit Biotinylation Detection Kit

Highly Sensitive, rapid, robust screening kit for Biotin
Useful for detecting and quantifying biotin in biological samples
Adaptable for detecting biotin in food samples