Postmeiotic Segregation Increased 2 (PMS2) is a DNA repair protein involved in mismatch repair. Mutations and deficiencies in the PMS2 gene have been linked to microsatellite instability, and malignancies such as hereditary nonpolyposis colorectal cancer and endometrial cancer. Expression levels of the PMS2 protein may be useful as a screening tool for Lynch syndrome after a colorectal cancer diagnosis. Anti-PMS2 is recommended to be used as part of a panel along with antibodies against MLH1, MSH2, and MSH6.
Nucleic acid testing (NAT) is the method of choice for detection and quantification of a wide range of micro organisms. Primerdesign manufactures and supplies high quality quantitative real-time PCR kits for the detection and simultaneous quantification of numerous significant pathogens . A copy number standard curve is provided for quantification and an the internal extraction template (DNA or RNA), controls for the quality of the nucleic acid extraction and eliminates false negative results.
The kit is designed with the broadest possible detection profile to ensure that all clinically relevant strains and subtypes are detected. Target sequences are selected by working with data from key opinion leaders in the field. Multiple sequence alignments and unprecedented real-time PCR expertise in design and validation ensure the best possible kit.
Details of the target and priming specificity are included in the individual handbooks above.
Packaged, optimised and ready to use. Expect Better Data.
Exceptional value for money
Rapid detection of all clinically relevant subtypes
Positive copy number standard curve for quantification
Highly specific detection profile
High priming efficiency
Broad dynamic detection range (>6 logs)
Sensitive to < 100 copies of target
Accurate controls to confirm findings
50 & 150 reactions
These kits provide a convenient and rapid method for the isolation of microorganisms from water samples. The kits allows for the rapid isolation and purification of total RNA and DNA simultaneously from the microorganisms found in different types of water samples. The total RNA and DNA (including genomic DNA) are isolated from all the microorganisms found in the water, including bacteria, fungi and algae without the use of any inhibitory organic substances The kit purifies genomic DNA, and all sizes of RNA, from large mRNA and ribosomal RNA down to microRNA (miRNA) and small interfering RNA (siRNA). The purified RNA and DNA are highly concentrated, and can be used directly in a number of downstream applications including PCR, qPCR, RT-PCR, qRT-PCR, Northern blotting, Southern blotting and sequencing reactions.
Water RNA/DNA Purification Kit (Spin Column + Filters Kits)
These kits are available with 0.45 μm or 0.22 μm filter formats for capturing microorganisms present in the water.
Water RNA/DNA Purification Kit (Spin Column)
This kit is sold without filters columns.
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| Kit Specifications | |
| Minimum Water Input | 10 mL |
| Maximum Water Input | 100 mL |
| Maximum Filter Column Loading Volume | 20 mL |
| Maximum Spin Column Loading Volume | 650 µL |
| Elution Volume | 100 µL |
| Time to Complete 10 Purifications | 45 minutes |
Storage Conditions and Product Stability
All solutions should be kept tightly sealed and stored at room temperature. This kit is stable for 2 years from the date of shipment.
| Component | Cat. 26400 (25 preps) | Cat. 26450 (25 preps) | Cat. 26480 (50 preps) |
|---|---|---|---|
| Lysis Buffer E | 15 mL | 15 mL | 2 x 15 mL |
| Wash Solution A | 18 mL | 18 mL | 38 mL |
| Enzyme Incubation Buffer B | 6 mL | 6 mL | 6 mL |
| Elution Buffer H | 6 mL | 6 mL | 6 mL |
| Mini Spin Columns | 25 | 25 | 50 |
| Filter Columns (0.22 μL) | 25 | – | – |
| Filter Columns (0.45 μL) | – | 25 | – |
| Bead Tubes | 25 | 25 | 50 |
| Collection Tubes | 25 | 25 | 50 |
| Elution Tubes (1.7 mL) | 25 | 25 | 50 |
| Product Insert | 1 | 1 | 1 |
Propargyl-PEG4-alcohol is a Click Chemistry reagent that can react with azide-bearing compounds or biomolecules; copper is needed as a catalyst. The PEG units help increase the solubility of the molecule in aqueous media. Reagent grade, for research purpose. Please contact us for GMP-grade inquiries.
Propargyl-PEG4-alcohol is a Click Chemistry reagent that can react with azide-bearing compounds or biomolecules; copper is needed as a catalyst. The PEG units help increase the solubility of the molecule in aqueous media. Reagent grade, for research purpose. Please contact us for GMP-grade inquiries.
