
Description
Specifications
Clone | IHC659 |
Source | Mouse Monoclonal |
Positive Control | Seminoma, Dysgerminoma |
Dilution Range | 1:200 |
Sal-Like Protein 4 (SALL4), is a zinc finger transcription factor found in germ cells and human blood progenitor cells, with functional involvement in modulating Oct-4 to maintain embryonic stem cell pluripotency. SALL4 is a useful marker for acute myeloid leukemia, B-cell acute lymphocytic leukemia, intratubular germ cell neoplasia, seminomas/dysgerminomas, and yolk sac tumors (both pediatric and postpubertal). Anti-SALL4 is used to detect embryonal carcinomas, hepatocellular carcinoma (HCC), gliomas, ovarian primitive germ-cell tumors, choriocarcinomas, spermatogonia, teratoma, gastric cancer, breast cancer, and lung cancer. Expression of SALL4 is often associated with poor prognosis in HCC, and with metastasis in endometrial cancer, colorectal carcinoma, and esophageal squamous cell carcinoma.
Clone | IHC659 |
Source | Mouse Monoclonal |
Positive Control | Seminoma, Dysgerminoma |
Dilution Range | 1:200 |
Norgen’s Saliva/Swab RNA Purification Kits provide a rapid method for the purification of total RNA from non-preserved saliva and nasal/throat swabs, and from preserved saliva collected on Norgen’s Saliva RNA Collection and Preservation Devices (Cat. RU53800) or preserved swabs collected in Norgen’s Total Nucleic Acid Preservative Tubes (Cat. 69200). Purification is based on using Norgen’s proprietary resin separation matrix. RNA is preferentially purified from other cellular components such as proteins, without the use of phenol or chloroform. The kit allows the purification of total RNA, including viral and bacterial RNA, irrespective of size or GC content. The purified RNA is eluted in an Elution Solution that is compatible with all downstream applications including PCR, qPCR, methylation-sensitive PCR and Southern Blot analysis, microarrays, and NGS.
This kit offers a range of elution volumes, with a minimum elution volume of 75 μL and a maximum elution volume of 100 μL. Complete 10 purifications in as little as 15 minutes.
High throughput 96-well plate format for rapid purifications with very consistent well-to-well RNA isolation. Purified RNA is suitable for a variety of downstream applications , including Small RNA Sequencing. Find out more information about Norgen’s NGS Services.
Figure 1 / 8
Click for expanded view
Kit Specifications | |
Sample Volume Range | 250 μL |
Size of RNA Purified | All sizes, including small RNA(<200 nt) |
Minimum Elution Volume | 50 μL |
Maximum Elution Volume | 100 μL |
Time to Complete 10 Purifications | 15 – 20 minutes |
Average Yield | ≥ 1 μg **Varies from sample to sample |
Storage Conditions and Product Stability
All solutions should be kept tightly sealed and stored at room temperature. This kit is stable for 2 years after the date of shipment.
Kit Component | Cat. 69100 (50 preps) | Cat. 69300 (192 preps) |
---|---|---|
Lysis Buffer A | 30 mL | 100 mL |
Solution WN | 18 mL | 55 mL |
Wash Solution A | 18 mL | 2 x 38 mL |
Elution Solution A | 6 mL | 20 mL |
Mini Spin Columns | 50 | – |
Collection Tubes | 50 | – |
Elution Tubes (1.7 mL) | 50 | – |
96-Well Isolation Plate | – | 2 |
96-Well Collection Plate | – | 2 |
96-Well Elution Plate | – | 2 |
Adhesive Tape | – | 4 |
Product Insert | 1 | 1 |
K-PHYTASE
SKU: 700004328
100 assays per kit
Content: | 100 assays per kit |
Shipping Temperature: | Ambient |
Storage Temperature: | Short term stability: 2-8oC, Long term stability: See individual component labels |
Stability: | > 2 years under recommended storage conditions |
Analyte: | Phytase |
Assay Format: | Spectrophotometer |
Detection Method: | Absorbance |
Wavelength (nm): | 360 |
Signal Response: | Increase |
Linear Range: | 0.1 to 10 μg of phosphate per assay |
Limit of Detection: | 1.5 U/L |
Reproducibility (%): | < 7% |
Reaction Time (min): | ~ 30 min |
Application examples: | Animal feeds, phytase activity in cereal, fungal and bacterial phytases. |
Method recognition: | Novel method |
The Phytase Assay Kit is a simple, quantitative method which can be used to measure phytase activity. Analysis is based on the hydrolysis of phytic acid by phytase and quantitative measurement of the phosphate released. Results are measured using a standard UV-VIS spectrophotometer and do not require the creation of a standard curve. The Phytase method can be used to measure phytase activity in cereal, fungal and bacterial phytases, and can also be used for the analysis of phytase in animal feed samples.
View our complete list of assay kits for enzyme activities.
Advantages
83, On-nut 88/2 Prawet Sub-district, Prawet District, Bangkok, 10250, Thailand
Tel : 081-875-1869 , 02-328-7179
Email : hej@a3p-scientific.com
Copyright © 2024 A3P Scientific Co., Ltd. All rights reserved. Web by Mountain Studio
Privacy Policy | Terms of Use | Site Map