
Description
Specifications
Clone | IHC010 |
Source | Mouse Monoclonal |
Positive Control | Melanoma, Skin Melanocytes |
Dilution Range | 1:200 |
SRY (Sex Determining Region Y)-Box 10 (SOX-10), also known as transcription factor SOX-10, is a nuclear transcription factor that acts in regulation of embryonic development and in the specification and differentiation of cells of melanocytic lineage. SOX-10 is diffusely expressed in neurofibromas and schwannomas, and mutations in the SOX-10 gene are linked to Waardenburg-Shah and Waardenburg-Hirschsprung disease. Anti-SOX-10 has been shown to be sensitive for conventional, spindled, and desmoplastic melanoma, and has been used to detect metastatic melanoma and nodal capsular nevus in sentinel lymph nodes.
Clone | IHC010 |
Source | Mouse Monoclonal |
Positive Control | Melanoma, Skin Melanocytes |
Dilution Range | 1:200 |
This kit is used for extracting total viral nucleic acid from non-cell/low cell content biological samples such as body fluid, serum, plasma, immersion solution, tissue homogenate supernatant, culture supernatant, etc., the extracted products can be used for clinical in vitro detection.
Specifications
Features | Specifications |
Main Functions | Extract viral RNA/DNA from 200μl plasma/serum samples |
Applications | RT-PCR,PCR,NGS |
Products | Viral DNA / RNA, body cell DNA / RNA |
Purification method | Mini spin column |
Purification technology | Silica technology |
Process method | Manual (centrifugation or vacuum) |
Sample type | Plasma, serum, effusion, urine, fecal suspension supernatant |
Sample amount | 200μl |
Elution volume | ≥30μl |
Time per run | |
Liquid carrying volume per column | 800μl |
Binding yield of column | 100μg |
This product is based on silica gel purification. The sample is lysed and digested with lysate and protease, DNA/RNA is released into the lysate. Transfer to an adsorption plate and filter column. DNA/RNA is adsorbed on the membrane, while protein is not adsorbed and is removed with filtration. After washing proteins and other impurities, DNA / RNA was finally eluted with low-salt buffer (10 MmTris, pH 8.0).
Advantages
Kit Contents
Contents | IVD4175 |
Purification Times | 100 Preps |
HiPure Viral Column | 100 |
2ml Collection Tubes | 100 |
PK/Carrier RNA | 50 mg/310μg |
Protease Dissolve Buffer | 5 ml |
Buffer VLE | 42 ml |
Buffer CE | 60 ml |
RNase Free Water | 15 ml |
Storage and Stability
This kit is shipped and stored at room temperature and is valid for 12 months.
This kit is used for extracting total viral nucleic acid from non-cell/low cell content biological samples such as body fluid, serum, plasma, immersion solution, tissue homogenate supernatant, culture supernatant, etc., the extracted products can be used for clinical in vitro detection.
K-GLNAM
SKU: 700004295
50 assays of each (manual) / 500 assays of each (microplate)
Content: | 50 assays of each (manual) / 500 assays of each (microplate) |
Shipping Temperature: | Ambient |
Storage Temperature: | Short term stability: 2-8oC, Long term stability: See individual component labels |
Stability: | > 2 years under recommended storage conditions |
Analyte: | Ammonia, L-Glutamine |
Assay Format: | Spectrophotometer, Microplate |
Detection Method: | Absorbance |
Wavelength (nm): | 340 |
Signal Response: | Decrease |
Linear Range: | 1 to 40 µg of L-glutamine per assay |
Limit of Detection: | 0.54 mg/L (L-glutamine), 0.06 mg/L (ammonia) |
Reaction Time (min): | ~ 10 min |
Application examples: | Cell culture media and cultures, dietary supplements, vegetables and other materials (e.g. biological samples, etc.). |
Method recognition: | Novel method |
This product has been discontinued.
The L-Glutamine/Ammonia (Rapid) test kit is a novel method for the specific, convenient, cost effective and rapid measurement and analysis of L-glutamine and ammonia in culture media/supernatants and other materials.
Note for Content: The number of manual tests per kit can be doubled if all volumes are halved. This can be readily accommodated using the MegaQuantTM Wave Spectrophotometer (D-MQWAVE).
See our full list of assay kits.
Advantages
The L-Glutamine/Ammonia (Rapid) test kit is a novel method for the specific, convenient, cost effective and rapid measurement and analysis of L-glutamine and ammonia in culture media/supernatants and other materials.
Sample type purification kit guide
The 16S V1-V3 Library Preparation Kit for Illumina consists of the reagents and components required for library preparation of the 16S V1-V3 amplicon libraries to be used for next-generation sequencing on Illumina platforms. All molecular reagents including primers, enzyme mixes, indexes, and buffers are provided. Instructions for PCR clean up with the AMPure XP Magnetic Beads (supplied by customer) are also included for rapid purification of nucleic acid products generated at two steps of the workflow. The library prep workflow could be used for purified DNA inputs from different sources including stool, soil, water, saliva, plant, urine, skin swab, vaginal swab, cheek swab, nasal swab, plasma/serum, tongue swab, gum swab, and others.
The 16S V1-V3 Library Preparation Kit for Illumina has a streamlined procedure that reduces the handling time such that the library prep procedure can be completed in approximately 4 hours (see diagram below). Input DNA is first subjected to targeted PCR to amplify the V1-V3 region of the DNA encoding 16S rRNA. The post-PCR reaction is then cleaned up using AMPure XP beads. Dual index primers are then added using a limited-cycle PCR. The indexed amplicons flanked by 5′ and 3′ barcoded adaptors are then cleaned using AMPure XP beads. The libraries are then ready for quantification, pooling and sequencing.
Figure 1 / 3
Click for expanded view
Minimum amount of starting material: | 2.5 µL of DNA (5 ng/µL) |
Time to complete library preparation: | 4 hours |
Storage Conditions and Product Stability
Norgen’s 16S V1-V3 Library Prep Kit for Illumina is shipped as one kit box (for the 24 prep kit) or two sub-component kits (for the 96 prep kit). All kits should be stored at -20°C upon arrival.
All kit components should remain stable for at least 1 year when stored at the specified storage conditions.
Step | Component | Cat. 70200 (24 preps) | Cat. 70210, 70220, 70230, 70240 (96 preps) |
---|---|---|---|
Amplicon PCR (PCR 1) | MGX Master Mix | 330 µL | 1,320 µL |
16S V1-V3 Primer Mix | 70 µL | 280 µL | |
Index PCR (PCR 2) | Indexing Master Mix | 660 µL | 2 x 1,320 µL |
N7xx Index Primer | 50 µL | 50 µL | |
S5xx Index Primer | 70 µL | 70 µL | |
PCR Clean-Up | Resuspension Buffer | 2 x 1,250 µL | 2 x 5,000 µL |
Nuclease-free water | 1,250 µL | 1 x 6,000 µL |
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