
Description
Specifications
Clone | IHC072 |
Source | Mouse Monoclonal |
Positive Control | Lung Adenocarcinoma |
Dilution Range | 1:200 |
Tumor-Associated Glycoprotein 72 (TAG-72) is a glycoprotein found on the surface of many cancer pathologies. Anti-TAG-72 can be useful for detecting some adenocarcinomas and non-neoplastic tissues. This diagnostic grade TAG-72 IVD antibody is useful for identifying adenocarcinomas in positive staining, but in mesotheliomas no staining is observed.
Clone | IHC072 |
Source | Mouse Monoclonal |
Positive Control | Lung Adenocarcinoma |
Dilution Range | 1:200 |
The JC virus (JCV) is a type of human polyomavirus and belongs to the family Papovaviridae. JCV is a double-stranded DNA virus, and is genetically similar to BK virus and SV40. The virus is very common in the general population and it is believed that most people acquire JCV in childhood or adolescence. Typically the infection is subclinical and no of consequence in children with healthy immune systems. The initial site of infection may be the tonsils or the gastrointestinal tract, and the virus then remains latent in the gastrointestinal tract. JCV can also infect the tubular epithelial cells in the kidneys, where it continues to reproduce, shedding virus particles in the urine. Also, JCV can cross the blood-brain barrier into the central nervous system. JCV is known to cause the usually fatal progressive multifocal leukoencephalopathy (PML) by destroying oligodendrocytes in the brain in immunodeficient or immunosuppressed individuals. However, it has not been established whether PML is the result of a primary infection with JCV in a person with impaired immunity or whether it follows reactivation of latent virus. JC virus is also the primary cause of nephropathy (kidney disease) in people who have received a kidney transplant and are on immunosuppressive therapy.
JCV TaqMan PCR Kit, 100 reactions
JCV TaqMan PCR Probe/Primer Set and Controls, 100 reactions
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Storage Conditions and Product Stability
All kit components can be stored for 2 years after the date of production without showing any reduction in performance.
All kit components should be stored at -20°C upon arrival. Repeated thawing and freezing (> 2 x) of the Master Mix and Positive Control should be avoided, as this may affect the performance of the assay. If the reagents are to be used only intermittently, they should be frozen in aliquots.
Component | Cat. TM37250 (100 preps) | Cat. TM37210 (100 preps) |
---|---|---|
MDx TaqMan 2X PCR Master Mix | 2 x 700 μL | – |
JCV Primer & Probe Mix | 280 μL | 280 μL |
JCV Positive Control | 150 μL | 150 μL |
Nuclease-Free Water (Negative Control) | 1.25 mL | 1.25 mL |
Product Insert | 1 | 1 |
The Norgen PCRSizer 100 bp DNA Ladder is prepared to ensure quality and batch-to-batch consistency. Our PCRSizer contains ten discrete fragments ranging from 100 bp to 1000 bp in 100 bp increments with a higher intensity reference band at 500 bp. This Ladder is ideal for PCR product size confirmation.
Contents:
1mL of premixed DNA ladder (0.5µg/10µL) in loading buffer (10mM EDTA, 10% glycerol, 0.015% bromophenol blue, and 0.17% SDS).
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PCR Sizer 100 bp DNA Ladder (Cat# 11400) – 100 loads
Ladder Properties:
• Ten discrete bands, ranging from 100 bp to 1,000 bp
• Higher intensity band at 500 bp for easy reference
Fragment | Size (bp) | Mass (ng) |
1 | 1000 | 78 |
2 | 900 | 70 |
3 | 800 | 62 |
4 | 700 | 55 |
5 | 600 | 47 |
6 | 500 | 78 |
7 | 400 | 31 |
8 | 300 | 24 |
9 | 200 | 31 |
10 | 100 | 24 |
Recommended Use:
Mix thoroughly. For best results, load 10µL of DNA ladder per well. For precise mass determination with a densitometer, stain gel after electrophoresis using 0.5µg/mL ethidium bromide for 30-40 minutes. The table above shows the size and mass for each band based on 10µL ladder per well.
Storage:
Stable at room temperature. For longer term storage, -20°C is recommended.
This ladder was standardized using 10µL of DNA per lane on a 0.8 cm thick, 13 x 15 cm, 1.0% agarose gel run in TAE buffer.
The MagPure Plasmid purification system uses the paramagnetic bead technology for high-throughput preparation of high-copy or low-copy plasmid DNA from E. coli cells. This kit also can be used with fosmid and BAC vector-based constructs. The system uses alkaline lysis followed by a MagPure purification to differentially bind plasmid DNA to paramagnetic beads. While the DNA is bound to the beads, contaminants can be rinsed away using a simple washing procedure. Because MagPure uses magnetic separation technology, the protocol does not require vacuum filtration. This makes kit extremely amenable to automation. Plasmid DNA purified with this system is most commonly used in Sanger Sequencing and PCR amplification.
Specifications
Features | Specifications |
Main Functions | Isolation up to 15μg endotoxin free plasmid DNA from 1-5ml bacterial culture |
Applications | Enzyme digestion, sequencing, PCR and labeling, etc. |
Purification technology | Magnetic beads technology |
Process method | Manual or automatic |
Sample type | Conventional plasmid, plasmid≤30KB |
Sample amount | 1-5ml |
Elution volume | ≥50μl |
Time per run | ≤80 minutes |
This product is based on the purification method of high binding magnetic particles. The sample is lysed and digested under the action of lysate and Lysozyme. DNA is released into the lysate. After adding magnetic particles and binding solution, DNA will be adsorbed on the surface of magnetic particles, and impurities such as proteins will be removed without adsorption.The adsorbed particles were washed with washing solution to remove proteins and impurities, washed with ethanol to remove salts, and finally DNA was eluted by Elution Buffer.
Advantages
Kit Contents
Contents | P181402 | P181403 | P181404 |
Purification Times | 100 Preps | 500 Preps | 5000 Preps |
RNase A | 10 mg | 30 mg | 2 x 160 mg |
Buffer P1 | 30 ml | 150 ml | 2 x 800 ml |
Buffer P2 | 30 ml | 150 ml | 2 x 800 ml |
Buffer LEN3 | 20 ml | 80 ml | 800 ml |
Buffer LN4 | 90 ml | 400 ml | 4 x 980 ml |
MagPure Particles | 3.5 ml | 17 ml | 3 x 60 ml |
Storage and Stability
RNase A and MagPure Particles should be stored at 2–8°C upon arrival. However, short-termstorage (up to 12 weeks) at room temperature (15–25°C) does not affect its performance. Theremaining kit components can be stored dry at room temperature (15–25°C) and are stable for atleast 18 months under these conditions.The entire kit can be stored at 2–8°C, but in this case buffers should be redissolved before use. Make sure that all buffers are at room temperature whenused. If any precipitates form in the buffers,warm at 37℃ to dissolve. After addition of RNase A, Buffer P1 is stable for 6 months when stored at
The MagPure Plasmid purification system uses the paramagnetic bead technology for high-throughput preparation of high-copy or low-copy plasmid DNA from E. coli cells. This kit also can be used with fosmid and BAC vector-based constructs. The system uses alkaline lysis followed by a MagPure purification to differentially bind plasmid DNA to paramagnetic beads. While the DNA is bound to the beads, contaminants can be rinsed away using a simple washing procedure. Because MagPure uses magnetic separation technology, the protocol does not require vacuum filtration. This makes kit extremely amenable to automation. Plasmid DNA purified with this system is most commonly used in Sanger Sequencing and PCR amplification.
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