Product Details

Sample Type:

Serum, Plasma, Whole Blood

Package Size:

48 Tests/Kit

Test Time:

5-20 Minutes

Target Disease:

African Swine Fever Virus

Product Name:

African Swine Fever Virus Isothermal Detetction Fluorescence Kit

Test Principle:

Nucleic Acid Detection

Target Species:

Pigs

High Light:

Livestock porcine detection kit

, 

porcine detection kit 48 Tests/Kit

, 

5-20 Minutes porcine test kit

Product Description

Livestock Disease Kit with African swine fever virus Test Kit for Nucleic Acid Detection 

Product parameters:

Kit composition (WLRE8208KIT 48T/Pack)
Composition	Content
E buffer	1 mL×2 Tubes
B buffer	150 μL×1Tube
Positive control template	100 μL×1Tube
Reagents	48T

Product features and advantages:

Product features and advantages
Features	Advantages
High sensitivity	1 copies/ml
Strong specificity	Specificity is superior to PCR
Short reaction time	20min
Polymorphic reagent	Liquid, freeze-dried powder, freeze-dried ball
Easy to operate	Few steps of liquid dispensing; It is even possible to add only samples and amplify to get the resultsDesign of primers and probe is simple
Easy to store and transport	It is best preserved at -20℃, and can be stored at room temperature for up to 30 days in a cool place away from light.The freeze-dried form has a long storage time and is convenient for transportation
Low requirements on equipment	Applicable to Applied Biosystems 7500, QuantStudio 3/5, QuantStudio 6/7 Flex fluorescence quantitative PCR instrument;Applicable to our WL-16-III and other isothermal fluorescence detector

Product application:

Application
Ultra-clean laboratory	African Swine Fever Virus Isothermal Detection
Indoor

Operation procedure and process:

Operation procedure and process:

Take out the liquid components of the kit in advance, thaw at room temperature, and shake to mix.

Prepare freeze-dried reagents according to the number of samples to be tested, negative and positive controls, and add 37.5 μL E buffer to each freeze-dried reagent.

Select the appropriate nucleic acid extraction method and reagent to extract the sample nucleic acid

Add 10 μL nucleic acid template to the reaction tube (the amount of template can be adjusted to be filled with sterile water; That is, 10 μL nucleic acid template plus sterile water), 10 μL positive control template was added to positive control, and 10 μL sterile water was added to negative control

Add 2.5μL B buffer to each reaction tube and close the tube cap (for multiple reactions, it is recommended to add B buffer to the inside of the tube cap)

Thoroughly mix the reaction tube upside and down for 8-10 times. After mixing, shake (or rapidly centrifuge) the reaction liquid to the bottom of the tube and transfer it to the amplification zone.

Performence Comparison Data:

No.	TT value	Template concentration	Result determination
1	4.2	10-1	Positive
2	5.5	10-2	Positive
3	10.2	10-3	Positive
4	17.0	10-4	Positive
5	12.0	10-5	Positive
6	–	10-6	Negative
7	–	10-7	Negative
8	Negative control		Negative

Support and Services:

Livestock Disease Kit Technical Support and Services

We provide technical support and services for our Livestock Disease Kit. We are committed to helping you get the most out of your product and ensuring your satisfaction with our products and services.

• Our technical support staff is available to answer any questions you may have about the product.
• We offer online tutorials and user guides to help you understand the product better.
• We provide technical assistance to help troubleshoot any issues you may be having with the product.
• We offer a warranty on our products.

If you have any questions or need technical support, please do not hesitate to contact us. We are here to help you make the most of your Livestock Disease Kit.

FAQ:

• Q: What diseases can Livestock Disease Kit detect?
  A: Livestock Disease Kit can detect different infectious diseases, such as Foot and Mouth Disease, Bluetongue Disease, Bovine Viral Diarrhea.
• Q: How does Livestock Disease Kit work?
  A: Livestock Disease Kit uses advanced MIRA technology to amplify the target DNA/RNA targent sequences , and detect the disease by analyzing the amplified sequences.
• Q: How long does it take to get results from Livestock Disease Kit?
  A: Livestock Disease Kit can give a result within 5-20 mins.
• Q: What type of sample is suitable for Livestock Disease Kit?
  A: Livestock Disease Kit is suitable for blood, serum, and tissue samples.
• Q: Does Livestock Disease Kit require professional equipment?
  A: No, Livestock Disease Kit does not require any professional equipment,can use with our isothermal equipment or pcr machine

Livestock Disease Kit with African swine fever virus Test Kit for Nucleic Acid Detection

Introduction

This product is specially designed for stool RNA extraction. The kit is suitable for extracting high-purity microbial or host cell RNA from ≤ 0.15g stool samples. The purified RNA can be directly used in RT-PCR and Northern hybridization.

Details

Principle

The Kit combines the speed and efficiency of silica-based technology with the convenient handling of magnetic particles for purification of total RNA. Samples are lysed and RNA is purified from lysates in one step through its binding to the silica surface of the particles in the presence of a chaotropic salt. The particles are separated from the lysates using a magnet and DNA is removed by treatment with RNase-free DNase. The magnetic particles are efficiently washed, and RNA is eluted in RNase-free water.

Kit Contents

Contents	R662601	R662602	R662603
Purification Times	48 Preps	96 Preps	5 x 96 Preps
MagPure RNA Particles	1.7 ml	4.0 ml	18 ml
DNase I	600 μl	2 x 600 μl	10 x 600 μl
DNase Buffer	30 ml	40 ml	200 ml
Buffer SPL	30 ml	60 ml	270 ml
Buffer PHC	30 ml	60 ml	270 ml
Buffer MCB*	9 ml	15 ml	75 ml
Buffer ALB3*	10 ml	20 ml	100 ml
Buffer GW1*	44 ml	66 ml	2 x 220 ml
Buffer RW2*	20 ml	50 ml	2 x 100 ml
RNase Free Water	10 ml	30 ml	120 ml
2ml Bead Tubes	48	96	5 x 96

Storage and Stability

MagPure RNA Particles should be stored at 2–8°C upon arrival. DNase I should be stored at -20°C. However, short-term storage (DNase I up to 1 weeks, MagPure RNA Particles up to 8 weeks) at room temperature (15–25°C) does not affect their performance. The remaining kit components can be stored at room temperature (15–25°C) and are stable for at least 18 months under these conditions.

This product is specially designed for stool RNA extraction. The kit is suitable for extracting high-purity microbial or host cell RNA from ≤ 0.15g stool samples. The purified RNA can be directly used in RT-PCR and Northern hybridization.

Permagen’s 16 x 1.5 mL Microfuge Tube Magnetic rack is designed for magnetic bead separations from up to 16 tubes

Contains the strongest magnets out of all of our Microfuge racks

Tubes are pushed 14mm between centers allowing the use of an 8-channel, adjustable head pipette. 8 tubes per side also make for easy transition to and from microplates

Accommodates many common 1.5 mL Microcentrifuge and some 2.0 mL tubes 

Tubes are angled and beads will be pulled to back wall allowing easy aspiration and tip tracking down the front wall of the tubes without disturbing bead pellet

SKU #

MSR16

Features

• 14 mm tube spacing for adjustable multi-channel pipettes
• Aluminum alloy design for years of trouble-free use
• Fast magnetic bead separations
• Held to tighter tolerances for more consistent results
• Pulls beads to side of wells for easy tip tracking down front side
• Rubber feet to help prevent slippage
• Stable design
• Angled tube holes which allow the tubes to come close to magnets

Compatibility

• Up to sixteen 1.5 mL Microcentrifuge Tubes
• Some 2.0 mL Microcentrifuge Tubes
• Any Magnetic Beads

Volumes

Minimum Volume – .3 mL

Maximum Volume – 1.5 mL

Permagen’s 16 x 1.5 mL Microfuge Tube Magnetic rack is designed for magnetic bead separations from up to 16 tubes