Description
Specifications
| Clone | IHC969 |
| Source | Mouse Monoclonal |
| Positive Control | Thyroid Gland |
| Dilution Range | 1:200 |
Tau proteins are abundant in neurons of the central nervous system, and function in microtubule stability and organization. Defects in Tau functioning and aggregation of these proteins have been clinically linked to a number of neurodegenerative disorders including Alzheimer’s disease, Parkinson’s disease, Pick’s disease (PiD), progressive supranuclear palsy (PSP), cortical basal degeneration (CBD), and frontotemporal dementia with parkinsonism linked to chromosome 17 (FTDP-17).
| Clone | IHC969 |
| Source | Mouse Monoclonal |
| Positive Control | Thyroid Gland |
| Dilution Range | 1:200 |
500g
Escherichia coli is one of many species of bacteria living in the lower intestines of mammals, known as gut flora. When located in the large intestine, it assists with waste processing, vitamin K production, and food absorption. Discovered in 1885 by Theodor Escherich, a German pediatrician and bacteriologist, E. coli are abundant: the number of individual E. coli bacteria in the faeces that a human defecates in one day averages between 100 billion and 10 trillion. However, the bacteria are not confined to the environment, and specimens have also been located, for example, on the edge of hot springs. The E. coli strain O157:H7 is one of hundreds of strains of the bacterium that causes illness in humans.
E. coli are unable to sporulate. Thus, treatments which kill all active bacteria, such as pasteurization or simple boiling, are effective for their eradication, without requiring the more rigorous sterilization which also deactivates spores. As a result of their adaptation to mammalian intestines, E. coli grow best in vivo or at the higher temperatures characteristic of such an environment, rather than the cooler temperatures found in soil and other environments.
The enteric E. coli (EC) are divided on the basis of virulence properties into enterotoxigenic (ETEC – causative agent of diarrhea in humans, pigs, sheep, goats, cattle, dogs, and horses), enteropathogenic (EPEC – causative agent of diarrhea in humans, rabbits, dogs, cats and horses); enteroinvasive (EIEC – found only in humans), verotoxigenic (VTEC – found in pigs, cattle, dogs and cats); enterohaemorrhagic (EHEC – found in humans, cattle, and goats, attacking porcine strains that colonize the gut in a manner similar to human EPEC strains) and enteroaggregative E. coli (EAggEC – found only in humans).
E. coli O157:H7 was first recognized as a pathogen as a result of an outbreak of unusual gastrointestinal illness in 1982. The outbreak was traced to contaminated hamburgers, and the illness was similar to other incidents in the United States and Japan. The etiologic agent of the illness was identified as a rare O157:H7 serotype of Escherichia coli in 1983. This serotype had only been isolated once before, from a sick patient in 1975.
Exceptional value for money
Rapid detection of all clinically relevant subtypes
Positive copy number standard curve for quantification
Highly specific detection profile
High priming efficiency
Broad dynamic detection range (>6 logs)
Sensitive to < 100 copies of target
Accurate controls to confirm findings
These kits are able to isolate all sizes of circulating and exosomal RNA, including microRNA, without the use of phenol or chloroform. The slurry format provides an advantage over other available kits in that it does not require extension tubes for the purification of free-circulating and exosomal RNA from large sample volumes. RNA can be isolated from either fresh or frozen samples using this kit. This kit is suitable for the isolation of RNA from serum or plasma prepared from blood collected only on either EDTA or citrate. Plasma samples prepared from blood collected on heparin should not be used as heparin can significantly interfere with many downstream applications including RT-PCR. The purified plasma/serum free-circulating and exosomal RNA is eluted in an elution solution that is compatible with PCR, qPCR, methylation-sensitive reverse transcription qPCR, reverse transcription PCR, Northern blotting, RNase protection and primer extension, expression array assays, and NGS.
Free-circulating plasma and serum RNA can serve as both tumor- and fetal-specific markers for cancer detection and prenatal diagnosis. As well, free-circulating RNAs have the potential to provide biomarkers for other disease states. Free-circulating RNA in plasma or serum are usually present as short fragments of less than 1000nt, and free-circulating miRNA (21nt) can also be found in plasma and serum.
Plasma/Serum Circulating and Exosomal RNA Purification Kit (Slurry)
Norgen’s Plasma/Serum Circulating and Exosomal RNA Purification Kit (Slurry Format) provides a fast, reliable and simple procedure for isolating circulating RNA and exosomal RNA from plasma and serum samples ranging from 0.25 mL to 5 mL.
Plasma/Serum Circulating and Exosomal RNA Purification Mini Kit (Mini Slurry)
Norgen’s Plasma/Serum Circulating and Exosomal RNA Purification Mini Kit (Slurry Format) provides a fast, reliable and simple procedure for isolating circulating and exosomal RNA from plasma and serum samples ranging from 0.25 mL to 2 mL.
Plasma/Serum Circulating and Exosomal RNA Purification 96-Well Kit (High Throughput Slurry)
Norgen’s Plasma/Serum Circulating and Exosomal RNA Purification 96-Well Kit (Slurry Format) provides a high throughput method for isolating circulating and exosomal RNA from plasma and serum samples using either a vacuum manifold or centrifugation.
Plasma/Serum Circulating and Exosomal RNA Purification Maxi Kit (Maxi Slurry)
Norgen’s Plasma/Serum Circulating and Exosomal RNA Purification Maxi Kit (Slurry Format) provides a fast, reliable and simple procedure for isolating circulating RNA and exosomal RNA from various amounts of plasma/serum ranging from 2 mL to 5 mL.
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| Kit Specifications – 96-well | |
| Minimum Plasma/Serum Input | 0.25 mL |
| Maximum Plasma/Serum Input | 2 mL |
| Size of RNA Purified | All sizes, including microRNA |
| Time to Complete Purification | < 1 hour |
Cat.29500 Storage Conditions and Product Stability
All solutions should be kept tightly sealed and stored at room temperature. This kit is stable for 2 years after the date of shipment.
| Component | Cat. 51000 (50 preps) | Cat. 29500 (96 preps) | Cat. 42800 (50 preps) | Cat. 50900 (25 preps) |
|---|---|---|---|---|
| Slurry C1 | – | – | – | 6 mL |
| Slurry C2 | 12 mL | – | 12 mL | – |
| Slurry C3 | – | 20 mL | – | – |
| Lysis Buffer A | 2 x 130 mL | 4 x 100 mL | 2 x 130 mL | 2 x 130 mL |
| Wash Solution A | 38 mL | 2 x 38 mL | 38 mL | 18 mL |
| Elution Solution A | 6 mL | 20 mL | 6 mL | 6 mL |
| Mini Filter Spin Columns | 50 | – | 50 | 25 |
| 96-Well Filter Plate | – | 1 | – | – |
| Adhesive Tape | – | 1 | – | – |
| Collection Tubes | 50 | – | 50 | 25 |
| 96-Well Collection Plate | – | 1 | – | – |
| Elution Tubes (1.7 mL) | 50 | – | 50 | 25 |
| 96-Well Elution Plate | – | 1 | – | – |
| Product Insert | 1 | 1 | 1 | 1 |