Description
Specifications
| Clone | IHC697 |
| Source | Mouse Monoclonal |
| Positive Control | Colon Cancer |
| Dilution Range | 1:200 |
Thymidylate Synthase (TS) is a crucial enzyme responsible for the synthesis of 2′-deoxythymidine-5′-monophosphate (dTMP) a precursor for thymidylate which is necessary for DNA replication and repair from 2′-deoxyuridine-5′-monophosphate (dUMP). In terms of cancer, TS is an important target for cancer treatment as the inhibition of TS and therefore nucleotide synthesis necessary for cell growth has shown to be a vital part for successful treatment against colorectal, pancreatic and breast cancers.
| Clone | IHC697 |
| Source | Mouse Monoclonal |
| Positive Control | Colon Cancer |
| Dilution Range | 1:200 |
The MagPure Plasmid purification system uses the paramagnetic bead technology for high-throughput preparation of high-copy or low-copy plasmid DNA from E. coli cells. This kit also can be used with fosmid and BAC vector-based constructs. The system uses alkaline lysis followed by a MagPure purification to differentially bind plasmid DNA to paramagnetic beads. While the DNA is bound to the beads, contaminants can be rinsed away using a simple washing procedure. Because MagPure uses magnetic separation technology, the protocol does not require vacuum filtration. This makes kit extremely amenable to automation. Plasmid DNA purified with this system is most commonly used in Sanger Sequencing and PCR amplification.
Specifications
| Features | Specifications |
| Main Functions | Isolation up to 15μg endotoxin free plasmid DNA from 1-5ml bacterial culture |
| Applications | Enzyme digestion, sequencing, PCR and labeling, etc. |
| Purification technology | Magnetic beads technology |
| Process method | Manual or automatic |
| Sample type | Conventional plasmid, plasmid≤30KB |
| Sample amount | 1-5ml |
| Elution volume | ≥50μl |
| Time per run | ≤80 minutes |
This product is based on the purification method of high binding magnetic particles. The sample is lysed and digested under the action of lysate and Lysozyme. DNA is released into the lysate. After adding magnetic particles and binding solution, DNA will be adsorbed on the surface of magnetic particles, and impurities such as proteins will be removed without adsorption.The adsorbed particles were washed with washing solution to remove proteins and impurities, washed with ethanol to remove salts, and finally DNA was eluted by Elution Buffer.
Advantages
Kit Contents
| Contents | P181402 | P181403 | P181404 |
| Purification Times | 100 Preps | 500 Preps | 5000 Preps |
| RNase A | 10 mg | 30 mg | 2 x 160 mg |
| Buffer P1 | 30 ml | 150 ml | 2 x 800 ml |
| Buffer P2 | 30 ml | 150 ml | 2 x 800 ml |
| Buffer LEN3 | 20 ml | 80 ml | 800 ml |
| Buffer LN4 | 90 ml | 400 ml | 4 x 980 ml |
| MagPure Particles | 3.5 ml | 17 ml | 3 x 60 ml |
Storage and Stability
RNase A and MagPure Particles should be stored at 2–8°C upon arrival. However, short-termstorage (up to 12 weeks) at room temperature (15–25°C) does not affect its performance. Theremaining kit components can be stored dry at room temperature (15–25°C) and are stable for atleast 18 months under these conditions.The entire kit can be stored at 2–8°C, but in this case buffers should be redissolved before use. Make sure that all buffers are at room temperature whenused. If any precipitates form in the buffers,warm at 37℃ to dissolve. After addition of RNase A, Buffer P1 is stable for 6 months when stored at
The MagPure Plasmid purification system uses the paramagnetic bead technology for high-throughput preparation of high-copy or low-copy plasmid DNA from E. coli cells. This kit also can be used with fosmid and BAC vector-based constructs. The system uses alkaline lysis followed by a MagPure purification to differentially bind plasmid DNA to paramagnetic beads. While the DNA is bound to the beads, contaminants can be rinsed away using a simple washing procedure. Because MagPure uses magnetic separation technology, the protocol does not require vacuum filtration. This makes kit extremely amenable to automation. Plasmid DNA purified with this system is most commonly used in Sanger Sequencing and PCR amplification.
Heat sealing film for 96- and 384-well PCR microplates.
We are thrilled to announce the latest addition to our consumables range at 3CR Bioscience: heat sealing film for 96- and 384-well PCR plates.
This rigorously tested, permanent seal film complements our rigid frame 384-well PCR plates perfectly. Its superior clarity allows for easy sample visualisation and monitoring, ensuring excellent data quality.
Crafted from high-quality materials, the seal offers excellent chemical resistance and plate compatibility. Designed with durability, the film provides a secure seal with precise fit and strong adhesion, safeguarding sample integrity throughout amplification, re-cycling, and reading.
Both seals and our 384-well plates are ideal for automated, high-throughput PCR environments, including robotic handling. The film remains stable during thermal cycling in PCR machines and hydro cyclers, offering performance and convenience across various PCR workflows.
With this new addition, we reaffirm our commitment to delivering reliable, high-performance, and affordable products that empower scientists worldwide.
Contact us today to include a free sample of plate and seal in your next shipment!
Heat sealing film for 96- and 384-well PCR microplates.
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