endo-BCN-PEG8-acid is a BCN-containing crosslinker reagent. The terminal carboxylic acid is reactive with primary amine groups in the presence of activators (e.g. EDC, or HATU). The BCN group enable copper free click chemistry with azide -tagged biomolecules. The hydrophilic PEG spacer increases solubility in aqueous media. Reagent grade, for research purpose. Please contact us for GMP-grade inquiries.

endo-BCN-PEG8-acid is a BCN-containing crosslinker reagent. The terminal carboxylic acid is reactive with primary amine groups in the presence of activators (e.g. EDC, or HATU). The BCN group enable copper free click chemistry with azide -tagged biomolecules. The hydrophilic PEG spacer increases solubility in aqueous media. Reagent grade, for research purpose. Please contact us for GMP-grade inquiries.

Introduction

The MagPure Plasmid purification system uses the paramagnetic bead technology for high-throughput preparation of high-copy or low-copy plasmid DNA from E. coli cells. This kit also can be used with fosmid and BAC vector-based constructs. The system uses alkaline lysis followed by a MagPure purification to differentially bind plasmid DNA to paramagnetic beads. While the DNA is bound to the beads, contaminants can be rinsed away using a simple washing procedure. Because MagPure uses magnetic separation technology, the protocol does not require vacuum filtration. This makes kit extremely amenable to automation. Plasmid DNA purified with this system is most commonly used in Sanger Sequencing and PCR amplification.

Details

Specifications

Features	Specifications
Main Functions	Isolation up to 15μg endotoxin free plasmid DNA from 1-5ml bacterial culture
Applications	Enzyme digestion, sequencing, PCR and labeling,  etc.
Purification technology	Magnetic beads technology
Process method	Manual or automatic
Sample type	Conventional plasmid, plasmid≤30KB
Sample amount	1-5ml
Elution volume	≥50μl
Time per run	≤80 minutes

Principle

This product is based on the purification method of high binding magnetic particles. The sample is lysed and digested under the action of lysate and Lysozyme. DNA is released into the lysate. After adding magnetic particles and binding solution, DNA will be adsorbed on the surface of magnetic particles, and impurities such as proteins will be removed without adsorption.The adsorbed particles were washed with washing solution to remove proteins and impurities, washed with ethanol to remove salts, and finally DNA was eluted by Elution Buffer.

Advantages

• High purity – purified plasmid can be directly used in sequencing, enzyme digestion and PCR, etc.
• Fast – it takes only 80 minutes to complete the isolation
• High yield – up to 15μg plasmid can be binded in one column
• Economic – excellent cost effectiveness performance

Kit Contents

Contents	P181402	P181403	P181404
Purification Times	100 Preps	500 Preps	5000 Preps
RNase A	10 mg	30 mg	2 x 160 mg
Buffer P1	30 ml	150 ml	2 x 800 ml
Buffer P2	30 ml	150 ml	2 x 800 ml
Buffer LEN3	20 ml	80 ml	800 ml
Buffer LN4	90 ml	400 ml	4 x 980 ml
MagPure Particles	3.5 ml	17 ml	3 x 60 ml

Storage and Stability

RNase A and MagPure Particles should be stored at 2–8°C upon arrival. However, short-termstorage (up to 12 weeks) at room temperature (15–25°C) does not affect its performance. Theremaining kit components can be stored dry at room temperature (15–25°C) and are stable for atleast 18 months under these conditions.The entire kit can be stored at 2–8°C, but in this case buffers should be redissolved before use. Make sure that all buffers are at room temperature whenused. If any precipitates form in the buffers,warm at 37℃ to dissolve. After addition of RNase A, Buffer P1 is stable for 6 months when stored at

The MagPure Plasmid purification system uses the paramagnetic bead technology for high-throughput preparation of high-copy or low-copy plasmid DNA from E. coli cells. This kit also can be used with fosmid and BAC vector-based constructs. The system uses alkaline lysis followed by a MagPure purification to differentially bind plasmid DNA to paramagnetic beads. While the DNA is bound to the beads, contaminants can be rinsed away using a simple washing procedure. Because MagPure uses magnetic separation technology, the protocol does not require vacuum filtration. This makes kit extremely amenable to automation. Plasmid DNA purified with this system is most commonly used in Sanger Sequencing and PCR amplification.

Overview

• Detection kits for the EBV
• CE-IVD marked version available for in vitro diagnostic use
• Available in TaqMan format for analysis

Epstein-Barr Virus (EBV) is a member of the Herpes family of virus and is one of the most common viruses in humans. The virus occurs globally and causes infectious mononucleosis. Most individuals become infected with EBV and develop adaptive immunity, with the majority of adults between the ages of 35 and 40 having been infected. The virus has been implicated as having a primary role in multiple autoimmune diseases, several lymphoproliferative disorders and cancers, particularly Hodgkin’s disease and Burkitt’s lymphoma. Many children become infected with EBV once maternal antibody protection disappears, however these infections usually do not result in symptom development. During adolescence the virus will cause mononucleosis in up to 69% of infections

EBV TaqMan PCR Kit, 100 reactions

• Ready to use format, including Master Mix for the target and PCR control to monitor for PCR inhibition and validate the quality
• Specific Primer and Probe mix for the pathogen/virus/viroid of interest
• Primer and Probe mix
• Positive and negative control to confirm the integrity of the kit reagents

EBV TaqMan PCR Probe/Primer Set and Controls, 100 reactions

• Specific Primer/Probe mix and Positive Control for the pathogen/virus/viroid of interest
• Nuclease-free water
• Can be used together with Norgen’s PCR Master Mix (#28007) or customer supplied master mix

Details

Supporting Data

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Storage Conditions and Product Stability
All kit components can be stored for 2 years after the date of production without showing any reduction in performance.

All kit components should be stored at -20°C upon arrival. Repeated thawing and freezing (> 2 x) of the Master Mix and Positive Control should be avoided, as this may affect the performance of the assay. If the reagents are to be used only intermittently, they should be frozen in aliquots.

Component	Cat. TM41050 (100 preps)	Cat. TM41010 (100 preps)
MDx TaqMan 2X PCR Master Mix	2 x 700 μL	–
EBV Primer & Probe Mix	280 μL	280 μL
EBV Positive Control	150 μL	150 μL
Nuclease-Free Water (Negative Control)	1.25 mL	1.25 mL
Product Insert	1	1