Haemophilus influenzae

Overview

• Optimized for low input RNA, especially from bodily fluids such as plasma or serum at 1ng of RNA
• Simple and quick workflow: library could be prepared in less than 5 hours
• No gel purification for selected types of samples
• Protocol optimized for RNA isolated from different types of input, including liquid biopsies (blood, plasma, serum and urine)
• Complements Norgen’s Best-in-Class Total RNA (including microRNA) Purification Technology

The Small RNA Library Prep Kit for Illumina consists of all the reagents and components required to generate small RNA libraries to be used for next-generation sequencing on an Illumina platform. All molecular reagents including adaptors, primers, enzyme mixes and buffers are provided. A purification module is also provided for rapid purification of nucleic acid products generated at various steps of the workflow. The purification module utilizes Norgen’s patent resin technology which enhances recovery of desired library intermediates or final products. The library prep workflow could be used for different forms of input including purified total RNA or enriched small RNA, as well as RNA from low content inputs such as plasma, serum and urine.

Workflow

Details

Supporting Data

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Kit Specifications
Number of preps	24
Number of indices provided	12 (for 24 preps, indexes 1-24 or 25-48)
Time required to complete 6 libraries	As little as 5 hours
Input RNA required	As little as 50 pg

Storage Conditions and Product Stability
Some components require storage at -20°C, 4°C or room temperature. See individual components and box labels for storage conditions.

Step	Component	Cat. 64600 (24 preps)
3′ AdaptorLigation to Template RNA	3′ Adaptor	30 µL
	3′ Adaptor Ligation Master Mix	320 µL
	T4 RNA Ligase 2 (Truncated)	35 µL
5′ Adaptor Ligation	5′ Adaptor	30 µL
	5′ Adaptor Ligation Master Mix	320 µL
	T4 RNA Ligase 1	35 µL
cDNA Synthesis from Ligated RNA Product	Reverse Primer	30 µL
	cDNA Synthesis Master Mix	220 µL
	TruScript ReverseTranscriptase	35 µL
PCR Amplification	2x NGS PCR Master Mix	1.32 µL
	PCR Reverse Primer	81 µL
	Forward Index Primer	Included in Small RNA Library Prep Forward Index Primers (# 64640 or # 64610)
Size Selection	NGS MW Ladder	50 µL
	NGS Control Ladder	50 µL
	Loading Dye	300 µL
	Nuclease-Free Water	1.25 µL

Component	Cat. 63500 (75 preps)
Buffer RL	40 mL
Wash Solution A	38 mL (Reconstituted to 128 mL)
Elution Solution A	6 mL
Columns	75
Gel Filtration Columns	24
Collection Tubes	75
Elution Tubes	75
Product Insert	1

Attogene’s Organophosphate detection kit is in designed specifically to detect Organophosphate in liquid samples.

Description

Organophosphate compounds (OP) account for the largest class of rural and urban poisons in the world that are used to kill pests but can also be toxic to humans.  OPs cause toxicity by means of blocking the acetylcholinesterase enzyme (AchE).  The AChE-directed OPs react with a serine residue that is located at the catalytic site found within the AChE gorge. The OP targeted enzyme is no longer able to hydrolyze ACh, resulting in the buildup of ACh in the nerve synapse. This effect causes excessive excitation of the nerves, producing uncoordinated movements, tremors, paralysis and death.  Both synthetic and natural(Guanitoxin) organophosphates are dangerous to humans — exposure can lead to visual, coordination, muscular, and neurological deficiencies, and in some cases even to death.  In turn, exposure to OP is a significant public health concern which would significantly benefit from an improved detection platform.

Attogene’s Organophosphate detection kit is in designed specifically to detect Organophosphate in liquid samples.  For solid samples a simple sample preparation method is performed.  The ability to detect Organophosphate is performed is simple and sensitive.  The reaction uses a chromophore that can be detected by eye. In the presence of Organophosphate, the rate of chromophore production is reduced in a concentration dependent fashion.  The higher the concentration of Organophosphate the less color is produced.

Attogene’s Organophosphate detection kit is in designed specifically to detect Organophosphate in liquid samples.

Description 

AccuBand™ 100 bp+3K DNA Ladder II is composed of 14 individual DNA fragments, presenting 3k, 2k, 1.5k, 1.2k, 1k, 900, 800, 700 600, 500, 400, 300, 200 and 100 bp sharp bands respectively. This product contains 2 enhanced bands (1k and 500 bp) for easy band identification. AccuBand™ 100 bp+3K DNA Ladder II is ready-to-use, containing loading buffer with dual color tracking dyes (orange G and Xylene cyanol FF). AccuBand™ 100 bp+3K DNA Ladder II provides a sufficient amount of DNA for clear observation of all DNA bands ranging from 100 bp to 3 kb, either in agarose gel or in polyacrylamide gel electrophoresis.

Features

• Sharp bands
• Suitable for polyacrylamide gel electrophoresis
• Quick reference— enhanced bands 
• Ready-to-use— premixed with loading dye for direct loading
• Stable— room temperature storage over 6 months

Source 

Phenol extracted PCR products and dsDNA digested with specific restriction enzymes, equilibrated in 10 mM Tris-HCl (pH 8.0) and 10 mM EDTA.

Range 

100 ~ 3,000 bp 

Concentration 

54.4 µg/ 500 µl 

Recommended loading volume 

5 µl/ well

Storage

Room temperature for 6 months
4°C for 12 months 
-20°C for 36 months