HAM001 Agar Powder Technical

Cytoprep-1 bench top cytology centrifuge

Features:

1.Widely used in the field of biochemistry、biological products、pharmaceutical factory and laboratory.

2. Brushless DC motor for model Cytoprep-1, free maintenance, no powder pollution, quick in speed up and down.

3. Smooth in operation, low noise and small vibration.

4. Frame is 3 tiers protection steel jacket, and with the stainless steel chamber, inside protective steel lagging which is simple-compact, light weight.

5. Microprocessor control, it can control rotate speed, and relative RCF, digital display which indicates the speed, time, RCF.

6. Automatically electric lid lock, super speed、over temperature protection and imbalance protection.

7. Power: AC 110V/220V, 50/60HZ, speed range:0-2800rpm, speed accuracy:± 20rpm

8. Emergency locks under the bottom of the centrifuge which help you open the lid when power off.

9. The centrifuge body is made of high-quality steel, safe and reliable.

Cytoprep-1 Cytology Technical Parameters:

Introduction

Usages:

 For selective isolation and culture of Pseudomonas aeruginosa.

Principle:

Acetamidase bacteria can use acetamide as a carbon source and acetamide decomposition Alcaligenes; NaCl maintain osmotic balance; dipotassium phosphate and potassium dihydrogenphosphate as buffer; magnesium ions provide necessary growth ; agar medium as coagulant; phenol red as a pH indicator.

Formulation:

Acetamide 10g

NaCl 5g

Dipotassium hydrogen phosphate 1.39g

Potassium dihydrogen phosphate 0.73g

Magnesium sulfate 0.5g

Phenol red 0.012g

Agar 15g

Final pH 7.2 ± 0.2

Storage: Keep container tightly closed, store in a cool, dry place, away from bright light. Storage period of 3 years.

100g

Overview

• Purification and enrichment of intact plasma/serum exosomes for functional studies
• Isolate all sizes of RNA, including microRNA, irrespective of size or GC content, without bias
• Versatile plasma/serum input volume
• No phenol extractions, Proteinase K treatment, nor carrier RNA required
• No time-consuming ultracentrifugation, filtration nor special syringes required
• No precipitation reagents, nor overnight incubation required
• Compatible with plasma/serum from most species
• Pure exosomes are purified and are free from any other RNA-binding proteins
• Purification is based on spin column chromatography that uses Norgen’s proprietary resin
• The purified RNA is suitable for a variety of downstream applications, including Small RNA Sequencing. Find out more information on Norgen’s NGS services

Norgen’s Plasma/Serum Exosome and RNA Isolation Kits constitute all-in-one systems for the purification of exosomes and the sequential isolation of exosomal RNA from different plasma/serum sample volumes. The purification is based on spin column chromatography that employs Norgen’s proprietary resin. These kits are designed to isolate all sizes of RNA, including microRNA, irrespective of size or GC content, without bias. These kits provide a clear advantage over other available kits in that they do not require any special instrumentation, protein precipitation reagents, extension tubes, phenol/chloroform or protease treatments. The RNA isolated from the purified exosomes is free from any protein-bound circulating RNA and is of the highest integrity. The purified RNA can be used in a number of downstream applications including real time PCR, reverse transcription PCR, Northern blotting, RNase protection and primer extension, and expression array assays.

Norgen’s Plasma/Serum Exosome and RNA Isolation Mini Kit

For sample volumes ranging from 50 µL to 1 mL. This kit allows the user to elute into a flexible elution volume ranging from 50 µL to 100 µL.

Norgen’s Plasma/Serum Exosome and RNA Isolation Midi Kit

For sample volumes ranging from 1 mL to 4 mL. This kit allows the user to elute into a flexible elution volume ranging from 50 µL to 100 µL.

Norgen’s Plasma/Serum Exosome and RNA Isolation Maxi Kit

For sample volumes ranging from 4 mL to 10 mL. This kit allows the user to elute into a flexible elution volume ranging from 50 µL to 100 µL.

Details

Supporting Data

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*Please check page 4 of the product insert for the average yields and the common RNA quantification methods.

Storage Conditions and Product Stability
All solutions should be kept tightly sealed and stored at room temperature. This kit is stable for 2 years after the date of shipment. It is recommended to warm Lysis Buffer A for 20 minutes at 60°C if any salt precipitation is observed.

Important Note
This kit is suitable for the purification of exosomes from fresh or frozen serum or plasma prepared from blood collected on either EDTA or Citrate. Plasma samples prepared from blood collected on heparin should not be used as heparin can significantly interfere with many downstream applications such as RT-PCR.