5 tests Lateral Flow Assay designed for the detection of Human Anti Mouse Antibodies (HAMA) in human serum.
Detail
Name of Product
HAMA – Human Anti Mouse Antibodies Detection Kit
Catalog Number
MQHM Z
Short Info
Lateral Flow Assay designed for the detection of Human Anti Mouse Antibodies (HAMA) in human serum.
This product is only available in the EU!
Method/Platform
Lateral Flow
Range/Assay Sensivity
97%
Test Principle
The HAMA Detection Kit is an immunoassay designed for qualitative determination of Human Anti Mouse Antibodies in human serum.
HAMA from patient’s sample bind first to mouse antibodies which are conjugated to gold nanoparticles.
In a second step the HAMA will be caught by mouse IgG antibodies, coated on the Test Line (T) of the nitrocellulose membrane.
In the presence of the HAMA-conjugat-complex a band becomes visible at the location of the Test Line (T).
The surplus of gold particles continues to migrate through the membrane and is captured at the Control Line (C) by specific antibodies.
Color’s intensitiy of the test line is directly proportional to the HAMA concentration in the sample.
Brief Instructions
Storage
2-8°C
Components
HAMA Test Unit, Chase Buffer, Evaluation Cards
Other Products
[PS1000] FluoroStain™ Protein Fluorescent Staining Dye (Red, 1,000X), 1 ml
Product Info
Document
Product Info
Description
The FluoroStain™ Protein Fluorescent Staining Dye (Red, 1000×) is designed to substitute the common Coomassie Blue protein staining method, offering greater sensitivity and ease of operation. Unlike Coomassie Blue stain, the FluoroStain Protein Fluorescent Staining Dye binds to protein with high specificity, making destaining process an option rather than a requirement. With further reduction of background signals via destaining process, the FluoroStain™ is capable of achieving detection level parallel to silver staining without specialized imaging equipment, making it one of the most sensitive dyes available. In addition to its remarkable sensitivity, the FluoroStain™ Protein Fluorescent Staining Dye (Red, 1000×) brings a more reliable and safer user experience, since the stained gel can be visualized with blue-light illumination, avoiding the risk of skin/ eye damage caused by UV light. For best result, we suggest using B-BOX™ Blue Light LED epi-illuminator to visualize and analyze the gel stained with FluoroStain Protein Fluorescent Staining Dye (Red, 1000×). The FluoroStain™ Protein Fluorescent Staining Dye is compatible to the analysis of mass spectra, i.e. LC-MS/MS, MALDI-TOF, etc.
Spectral Characteristics
When it is bound with bovine serum albumin (BSA), the fluorescent emission of FluoroStain Protein Fluorescent Staining Dye can be excited by UV and blue light sources, with excitation peaks around 369 and 517 nm and emission at 605 nm. In absence of BSA, FluoroStain Protein Fluorescent Staining Dye shows ignorable fluorescence as compared with protein-bound form, therefore giving a clear background for photographic analysis.
These spectral characteristics made this fluorescent dye compatible with a wide variety of gel reading facilities, including UV/ blue light epi- and transilluminator, argon laser and mercury-arc lamp excitation gel scanners.
Storage
Protected from light -20°C for 24 months
Document
The FluoroStain™ Protein Fluorescent Staining Dye (Red, 1000×) is designed to substitute the common Coomassie Blue protein staining method, offering greater sensitivity and ease of operation. Unlike Coomassie Blue stain, the FluoroStain Protein Fluorescent Staining Dye binds to protein with high specificity, making destaining process an option rather than a requirement. With further reduction of background signals via destaining process, the FluoroStain™ is capable of achieving detection level parallel to silver staining without specialized imaging equipment, making it one of the most sensitive dyes available. In addition to its remarkable sensitivity, the FluoroStain™ Protein Fluorescent Staining Dye (Red, 1000×) brings a more reliable and safer user experience, since the stained gel can be visualized with blue-light illumination, avoiding the risk of skin/ eye damage caused by UV light. For best result, we suggest using B-BOX™ Blue Light LED epi-illuminator to visualize and analyze the gel stained with FluoroStain Protein Fluorescent Staining Dye (Red, 1000×). The FluoroStain™ Protein Fluorescent Staining Dye is compatible to the analysis of mass spectra, i.e. LC-MS/MS, MALDI-TOF, etc.
