Principle: Peptone provide carbon and nitrogen sources to meet the needs of bacterial growth; lactose are fermentable sugars; selenite, sodium hydrogen inhibit Gram-positive bacteria and gram-negative enterobacteria most non-Salmonella; phosphate-buffered agent; L- cystine as a reducing agent.
How to use: 1. Weigh 23g of the product , adding 1 L of distilled or deionized water , heated to boiling stirring until completely dissolved, dispensing flask, cooled to room temperature . 2. Pipette 10mL of pre-enrichment sample broth or 25mL and transferred species in the liquid sample flask in a sterile environment. 3.Place into incubator, cultured at 36 ± 1 for 18-24h. 4. Observe the results.
Quality control: Quality control strains were inoculated ,and cultured at 36 ± 1 for 18-24h ,results show as follows: strain name strain code growth feature Salmonella typhi CMCC (B) 50071 good red, cloudy Salmonella typhimurium CMCC (B) 50115 good red, cloudy Escherichia coli ATCC25922 — remain unchanged
Storage: Store in a dark, cool and dry place, tighten the cap immediately after use. Storage period of three years.
Other Products
Plasma/Serum Circulating DNA Purification Kits (Slurry Format)
Product Info
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Product Info
Overview
Isolate all sizes of circulating DNA from plasma and serum samples
Isolate Viral and Bacterial DNA
Versatile plasma and serum input volumes
Concentrate circulating DNA into small elution volumes
Different elution strategies depending on the downstream application
Isolate inhibitor-free circulating DNA for any application including PCR, qPCR, methylation-sensitive PCR
Purification is based on Norgen’s proprietary Silicon Carbide resin matrix
Norgen’s Plasma/Serum Circulating DNA Purification Kits (Slurry Format) provide efficient methods for the purification of fragmented free-circulating DNA from human plasma or serum. The kit is able to isolate all sizes of circulating DNA. The slurry format provides an advantage over other available kits in that it does not require extension tubes for the purification of free-circulating DNA from large sample volumes. DNA can be isolated from either fresh or frozen samples using this kit. The kit will also isolate viral and bacterial DNA from plasma/serum. Typical yields of purified free-circulating DNA will vary depending on the input sample (1-100ng/mL circulating DNA in human plasma), with more concentrated samples tending to yield more free-circulating nucleic acids. The purified, inhibitor-free plasma/serum free-circulating DNA is eluted in an elution solution that is compatible with PCR, qPCR, methylation-sensitive PCR and Southern Blot analysis.
Plasma/Serum Circulating DNA Purification Mini Kit (Slurry Format)
Norgen’s Plasma/Serum Circulating DNA Purification Mini Kit (Slurry Format) provides a fast, reliable and simple procedure for isolating circulating DNA from various amounts of plasma/serum ranging from 50 μL to 400 μL. Preparation time for a single sample is less than 30 minutes.
Plasma/Serum Circulating DNA Purification Midi Kit (Slurry Format)
Norgen’s Plasma/Serum Circulating DNA Purification Midi Kit (Slurry Format) provides a fast, reliable and simple procedure for isolating circulating DNA from various amounts of plasma/serum ranging from 400 μL to 2 mL. Preparation time for a single sample is less than 45 minutes.
Plasma/Serum Circulating DNA Purification Maxi Kit (Slurry Format)
Norgen’s Plasma/Serum Circulating DNA Purification Maxi Kit (Slurry Format) provides a fast, reliable and simple procedure for isolating circulating DNA from various amounts of plasma/serum ranging from 2 mL to 10 mL. Preparation time for a single sample is less than 45 minutes.
Propargyl-PEG4-amine has a propargyl group and an amine group. The amine group is reactive with carboxylic acids, activated NHS esters, carbonyls, etc. The propargyl group can form triazole linkage with azide-bearing biomolecules. Reagent grade, for research purpose. Please contact us for GMP-grade inquiries.
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Propargyl-PEG4-amine has a propargyl group and an amine group. The amine group is reactive with carboxylic acids, activated NHS esters, carbonyls, etc. The propargyl group can form triazole linkage with azide-bearing biomolecules. Reagent grade, for research purpose. Please contact us for GMP-grade inquiries.
For the cultivation of E. coli in fermentation and molecular genetic studies.
Principle and Interpretation
Peptone provides nitrogen . Vitamins (including B vitamins) and certain trace elements are provided by yeast extract. Glucose provides carbon.Sodium ions for transport and osmotic balance are provided by sodium chloride. Agar is the solidifying agent.
Formulation
Ingredients
/liter
Peptone
10.0g
Yeast extract
5.0g
Sodium chloride
5.0g
Glucose
1.0g
Agar
15.0g
pH7.0±0.2 at 25°C
Preparation
Suspend 36g in 1 litre of distilled water. Bring to a boil to dissolve completely. Sterilize by autoclaving at 121°C for 15minutes.
Quality Control
Cultural characteristics observed after incubation at 35-37°C for 24 hours
Quality control strains
Growth
Escherichia coli ATCC25922
good
Storage and Shelf Life
2-30℃,Keep container tightly closed, avoid direct sunlight.
Use before expiry date on the label.
Precautions
1. When weighing the dehydrated medium, please wear masks to avoid causing respiratory system discomfort
2. Keep container tightly closed after using to prevent clumping.
Waste Disposal
Microbiological contamination was disposed by autoclaving at 121°C for 30 minutes.