Usages: For differentiating enteric bacteria based on urease activity by adding 40% sterile urea solution.
Principle: Peptone provides the carbon and nitrogen; maintain a balanced osmotic sodium chloride; potassium dihydrogen phosphate is buffers; decomposing bacteria urease urea medium, produce large amounts of ammonia, agar as medium coagulant.
Formulation (per liter): Peptone 1g Sodium chloride 5g Glucose 1g Ppotassium dihydrogen phosphate 2g Phenol red 0.012g Agar 12g Final pH7.2 ± 0.2
How to use: 1.Suspend 21g in 1L of distilled or deionized water. Heat with frequent agitation and boil to completely dissolve the powder. Distribute into flasks. Autoclave at 121 for 15 minutes. cooling to 50-55 and adding 40% urea solution. 2.Diluted and treated samples.
Storage: Store in a dark, cool and dry place, tighten the cap immediately after use. Storage period of three years.
2,5-Dioxopyrrolidin-1-yl 2-(prop-2-yn-1-yloxy)acetate is a Click Chemistry reagent with a propargyl group and an NHS ester group. The propargyl group can react with biomolecules containing azide group via copper catalyzed Click Chemistry reaction. The NHS ester is an amine reactive group which can be used for derivatizing peptides, antibodies, amine coated surfaces, etc. Reagent grade, for research use only.
Document
2,5-Dioxopyrrolidin-1-yl 2-(prop-2-yn-1-yloxy)acetate is a Click Chemistry reagent with a propargyl group and an NHS ester group. The propargyl group can react with biomolecules containing azide group via copper catalyzed Click Chemistry reaction. The NHS ester is an amine reactive group which can be used for derivatizing peptides, antibodies, amine coated surfaces, etc. Reagent grade, for research use only.
This kit is suitable for extracting total pathogen nucleic acid from a variety of clinical samples such as blood, serum, plasma, swab soaking solution, fluid accumulation and homogenate solution. This kit is designed to remove host cells background nucleic acid and enrich pathogen nucleic acid (including viral/bacterial/fungal DNA/RNA) from the sample. Purified DNA/RNA is ready for downstream applications such as PCR, virus detection, tNGS and other related experiments.
Details
Specifications
Features
Specifications
Main Functions
Extract Pathogen RNA/DNA from 0.5-1.5ml whole blood, plasma, serum, body fluid, homogenate suspension, culture solution, cell suspension, soaking solution or concentrate pathogen solution for tNGS application, remove host background nucleic acid.
Applications
Real Time PCR, biochip analysis, NGS
Products
Pathogen DNA / RNA
Purification method
Polydisperse magnetic beads
Purification technology
Magnetic beads technology
Process method
Manual or automatic
Sample type
whole blood, plasma, serum, body fluid, homogenate suspension, culture solution, cell suspension, soaking solution or concentrate pathogen solution
Sample amount
0.5 – 1.5 ml
Adaptive instrument
Nucleic acid extractor, pipetting workstation
Principle
This product is based on the purification method of high binding magnetic particles. The sample is lysed and digested under the action of lysate and Protease. After adding magnetic particles and binding solution, DNA/RNA will be adsorbed on the surface of magnetic particles, and impurities such as proteins will be removed without adsorption. The adsorbed particles were washed with washing solution to remove proteins and impurities, washed with ethanol to remove salts, and finally DNA/RNA was eluted by Buffer NFW.
Kit Contents
Contents
R667200C
R667202C
Purification Times
24 Preps
96 Preps
2ml Bead Tube (0.4g)
24
96
DNase I (Powder)
10 mg
15 mg
DNase Buffer
5 ml
20 ml
Protease Dissolve Buffer
3 ml
8 ml
Lysis Buffer LBX1
40 ml
180 ml
Buffer TL
5 ml
20 ml
Proteinase K
24 mg
120 mg
MagBind Particles N9
1.2 ml
5 ml
Buffer MLB
30 ml
120 ml
Buffer MW1*
13 ml
110 ml
Buffer MW2*
10 ml
50 ml
Buffer AVE
10 ml
20 ml
Storage and Stability
Proteinase K, DNase I powder and MagPure Particles N9 should be stored at 2–8°C upon arrival. However, short-term storage (up to 8 weeks) at room temperature (15–25°C) does not affect their performance. The remaining kit components can be stored at room temperature (15–25°C) and are stable for at least 18 months under these conditions.
Document
This kit is suitable for extracting total pathogen nucleic acid from a variety of clinical samples such as blood, serum, plasma, swab soaking solution, fluid accumulation and homogenate solution. This kit is designed to remove host cells background nucleic acid and enrich pathogen nucleic acid (including viral/bacterial/fungal DNA/RNA) from the sample. Purified DNA/RNA is ready for downstream applications such as PCR, virus detection, tNGS and other related experiments.
