Peptone, beef extract powder and yeast extract powder provide nitrogen, vitamins, minerals; lactose, dextrose into fermentable sugars, which produce acid when measured by the phenol red indicator, acid yellow, basic red; thiosulfate sodium can be reduced to some bacteria to hydrogen sulfide, to produce a black iron sulfide and iron salts; sodium chloride to maintain osmotic equilibrium; agar as medium coagulant.
Formulation(per liter):
Peptone 20g
Beef extract powder 3g
Yeast extract powder 3g
Lactose 10g
Dextrose 1g
Sodium chloride 5g
Ferric ammonium citrate 0.5g
Sodium thiosulfate 0.5g
Agar 12g
Phenol red 0.05g
Final pH 7.4 ± 0.2
How to use:
1.Suspend 54.5g in 1L of distilled water , stirring heated to boiling ,autoclave at 121 for 15 minutes.
2.Diluted and treated samples.
Quality control:
Item
The name and number of strain
Growth
Colony Color
1
Escherichia coli ATCC25922
Good
A/A
2
Proteus CMCC (B) 49027
Good
K/A
3
Salmonella typhimurium CMCC (B) 50115
Good
K/A
“A”Acid , “K” Alkality
Storage: Keep container tightly closed, store in a cool, dry place, away from bright light. Storage period of 3 years.
Other Products
D3148 HiPure Microbiome DNA Kit
Product Info
Document
Product Info
Introduction
This product provides an easy-to-use workflow for selective isolation of bacterial DNA from samples that are intrinsically rich in host DNA, such as body fluids or swabs. The method is specific for the identification of intact bacteria so it prevents false results due to nucleic acids from dead bacteria. The Kit allows isolation of enriched bacterial DNA suitable for a variety of applications, including qPCR and whole metagenome or 16S rRNA gene sequencing.
Details
Specifications
Features
Specifications
Main Functions
Isolation gDNA from biological sample and remove host DNA
Applications
PCR, southern blot and enzyme digestion, etc.
Purification method
Mini spin column
Purification technology
Silica technology
Process method
Manual (centrifugation or vacuum)
Sample type
Culture medium, swab, parasitic blood, tissue, sputum, etc.
This product is based on silica Column purification. This product efficiently depletes human and animal host DNA and yields enriched bacterial DNA. An optimized combination of mechanical and chemical lysis allows efficient disruption of bacterial cells. Target DNA is adsorbed on the membrane, while protein is not adsorbed and is removed with filtration. After washing proteins and other impurities, nucleic acid was finally eluted with low-salt buffer (10mm Tris, pH9.0, 0.5mm EDTA).
Advantages
High concentration – the host DNA is digested by dnase, without contamination of host DNA
High recovery – DNA can be recovered at the level of PG
Good repeatability – silica technology can obtain ideal results every time
Wide applicability – it can be used in blood, tissue, intestinal contents and other samples
Kit Contents
Contents
D314802
D314803
Purification Times
50 Preps
250 Preps
Hipure DNA Mini Columns I
50
2 x 125
2ml Collection Tubes
50
2 x 125
2ml beads Tubes
50
250
Buffer DRB
15 ml
60 ml
Buffer ES
6 ml
30 ml
Reagent DX
0.5 ml
1 ml
Buffer DL
30 ml
120 ml
Buffer GW1
22 ml
110 ml
Buffer GW2
12 ml
50 ml
DNase I (Powder)
6 mg
30 mg
Proteinase K
24 mg
120 mg
Protease Dissolve Buffer
5 ml
15 ml
Buffer AE
15 ml
60 ml
Storage and Stability
DNase I and Proteinase K should be stored at 2–8°C upon arrival. However, short-term storage (up to 12 weeks) at room temperature (15–25°C) does not affect their performance. The remaining kit components can be stored dry at room temperature (15–25°C) and are stable for at least 18 months under these conditions. The entire kit can be stored at 2–8°C, but in this case buffers should be redissolved before use. Make sure that all buffers are at room temperature when used.
Document
This product provides an easy-to-use workflow for selective isolation of bacterial DNA from samples that are intrinsically rich in host DNA, such as body fluids or swabs. The method is specific for the identification of intact bacteria so it prevents false results due to nucleic acids from dead bacteria. The Kit allows isolation of enriched bacterial DNA suitable for a variety of applications, including qPCR and whole metagenome or 16S rRNA gene sequencing.
The Kit is designed for purification of total RNA, including miRNA and other small RNA molecules (18nt), from cultured cells and various animal and human tissues, including difficult-to-lyse tissues samples.
Details
Specifications
Features
Specifications
Main Functions
Isolation total RNA and miRNA from cell and tissue without MagZol reagent
Applications
RT-PCR, Northern Blot, poly A+purification, nucleic acid protection and in vitro translation
Purification method
Mini spin column
Purification technology
Silica technology
Process method
Manual (centrifugation or vacuum)
Sample type
Cell, Animal tissue, Plant tissue
Sample amount
Cells: ≤ 5 x 10^6, Animal tissue:<10mg
Elution volume
≥30μl
Time per run
≤40 minutes
Liquid carrying volume per column
800µl
Binding yield of column
100µg
Principle
Biological samples are first lysed and homogenized in a highly denaturing guanidine isothiocyanate-containing buffer, which immediately inactivates DNases and RNases to ensure isolation of intact DNA and RNA. The lysate is then passed through a Mini spin column. This column, in combination with the high-salt buffer, allows selective and efficient binding of genomic DNA. Flow-through from the column is digested by Proteinase K in the presence of ethanol. This optimized digestion, together with the subsequent addition of further ethanol, allows appropriate binding of total RNA, including miRNA, to the column. Contaminants are efficiently washed away and high-quality RNA is eluted.
Advantages
High quality – one step RNA extraction reagent combined with silica gel column can obtain the highest concentration
Fast – several samples can be extracted in 40 minutes
High applicability – samples including animals, plants, bacteria, cells, etc.
High concentration – efficiently remove macromolecular RNA, enrich small RNA and improve sensitivity
Safe – no phenol chloroform extraction and no use of Trizol reagent
Kit Contents
Contents
R431102
R431103
Purification Times
50 Preps
250 Preps
HiPure RNA Mini Columns
100
2 x 250
2ml Collection Tubes
100
2 x 250
Proteinase K
48 mg
240 mg
Protease Dissolve Buffer
5 ml
15 ml
Buffer RLC
40 ml
200 ml
Buffer RWC
20 ml
80 ml
Buffer RW2*
20 ml
2 x 50 ml
RNase Free Water
10 ml
60 ml
Storage and Stability
Proteinase K should be stored at 2-8°C upon arrival. However, short-term storage (up to 12 weeks) at room temperature (15-25°C) does not affect their performance. The remaining kit components can be stored at room temperature (15-25°C) and are stable for at least 18 months under these conditions.
Document
The Kit is designed for purification of total RNA, including miRNA and other small RNA molecules (18nt), from cultured cells and various animal and human tissues, including difficult-to-lyse tissues samples.
endo-BCN-PEG4-acid is a click chemistry reagent with a BCN group witha terminal carboxylic acid (CO2H). The terminal carboxylic acid is reactive with primary amine groups in the presence of activators (e.g. EDC, or HATU) to form a stable amide bond. The BCN group can react with azide -tagged compound or biomolecules. The hydrophilic PEG spacer increases solubility in aqueous media. Reagent grade, for research purpose. Please contact us for GMP-grade inquiries.
Document
endo-BCN-PEG4-acid is a click chemistry reagent with a BCN group witha terminal carboxylic acid (CO2H). The terminal carboxylic acid is reactive with primary amine groups in the presence of activators (e.g. EDC, or HATU) to form a stable amide bond. The BCN group can react with azide -tagged compound or biomolecules. The hydrophilic PEG spacer increases solubility in aqueous media. Reagent grade, for research purpose. Please contact us for GMP-grade inquiries.
