For cultivating and enriching nofastidious bacteria ,and for evaluating the efficacy of disinfection.
Formula (per liter)
Peptone—————10.0g Beef Extract———–3.0g Sodium Chloride—–5.0g Final pH———–7.4 ± 0.2
How to use
1.Suspend 18g in 1L of distilled water , stirring heated to boiling to completely dissolve ,autoclave at 121ºC for 15 minutes. 2.Diluted and treated samples.
Storage
Keep container tightly closed, store in a cool, dry place, away from bright light. Storage period of 3 years.
Other Products
HCM011 Selenite Cystine Broth (SC)
Product Info
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Product Info
Introduction
Usages: For selective enrichment of Salmonella.
Principle: Peptone provide carbon and nitrogen sources to meet the needs of bacterial growth; lactose are fermentable sugars; selenite, sodium hydrogen inhibit Gram-positive bacteria and gram-negative enterobacteria most non-Salmonella; phosphate-buffered agent; L- cystine as a reducing agent.
How to use: 1. Weigh 23g of the product , adding 1 L of distilled or deionized water , heated to boiling stirring until completely dissolved, dispensing flask, cooled to room temperature . 2. Pipette 10mL of pre-enrichment sample broth or 25mL and transferred species in the liquid sample flask in a sterile environment. 3.Place into incubator, cultured at 36 ± 1 for 18-24h. 4. Observe the results.
Quality control: Quality control strains were inoculated ,and cultured at 36 ± 1 for 18-24h ,results show as follows: strain name strain code growth feature Salmonella typhi CMCC (B) 50071 good red, cloudy Salmonella typhimurium CMCC (B) 50115 good red, cloudy Escherichia coli ATCC25922 — remain unchanged
Storage: Store in a dark, cool and dry place, tighten the cap immediately after use. Storage period of three years.
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