For cultivating of bacteria.And sterility test of drugs and biological products.
Principle:
Tryptone, peptone and yeast extract multivalent powder provides a nitrogen source, vitamins, and growth factors; sodium chloride to maintain osmotic balance; glucose carbon source; dipotassium hydrogen phosphate as a buffering agent.
Formulation(per liter):
Pancreatic Digest of Casein 17g
Papaic Digest of Soybean 3g
Sodium chloride 5g
Dipotassium hydrogen phosphate 2.5g
Glucose Monohydrate 2.5g
Final pH 7.3±0.2
How to use:
1.Suspend 30g in 1L of distilled water , stirring heated to boiling to completely dissolve ,autoclave at 121 for 15 minutes.
2.Diluted and treated samples.
Storage: Keep container tightly closed, store in a cool, dry place, away from bright light. Storage period of 3 years.
Other Products
Mal-amido-PEG5-alkyne
Product Info
Document
Product Info
Mal-amido-PEG5-alkyne is a PEG linker containing a maleimide group and an alkyne. The hydrophilic PEG spacer increases solubility in aqueous media. The alkyne group can react with azide-bearing compounds or biomolecules via copper catalyzed azide-alkyne Click Chemistry to yield a stable triazole linkage. The maleimide group will react with a thiol group to form a covalent bond, enabling the connection of biomolecule with a thiol. Reagent grade, for research purpose. Please contact us for GMP-grade inquiries.
Document
Mal-amido-PEG5-alkyne is a PEG linker containing a maleimide group and an alkyne. The hydrophilic PEG spacer increases solubility in aqueous media. The alkyne group can react with azide-bearing compounds or biomolecules via copper catalyzed azide-alkyne Click Chemistry to yield a stable triazole linkage. The maleimide group will react with a thiol group to form a covalent bond, enabling the connection of biomolecule with a thiol. Reagent grade, for research purpose. Please contact us for GMP-grade inquiries.
[CK1000] Champion™ E. coli Transformation Kit, 200 Rxn
Product Info
Document
Product Info
Description
Champion™ E. coli Transformation Kit provides an easy method for rapid preparation of chemically competent cells with high transformation efficiency from fresh culture, overnight culture, or even directly from bacterial colonies on the plate. The competent cell preparation method eliminates the requirement of time-wasting wash step. In addition, preparation of competent cells from overnight culture or directly from bacterial colonies provides flexibility to cloning experiment. The resultant competent cells can be immediately used or stored at -70°C for one year. This kit includes a specialized SMO-Broth™ medium and a unique Champion™ CC Buffer for culturing and preparing competent cells efficiently. Following the simple and quick competent cell preparation protocol from fresh culture, the transformation efficiency is typically ranged from 108–109 cfu/μg transformants/μg of pUC19 plasmid DNA, but varies depending on the E. coli strains. The resultant competent cells can be further transformed using time-saving transformation protocol, eliminating the requirement of heat-shock and recovery steps.
Features
Flexible– fresh culture, overnight culture, 4°C stored liquid culture or even colonies on agar plate can be used for transformation.
Fast and Easy– only few steps for preparation; suitable for time-saving transformation
High efficiency– up to 109 cfu/μg
Personalization– suitable for most E. coli strains
Kit Contents
Component
Volume
Champion™ CC Buffer
20 ml
SMO-Broth™
100 ml x 2
pUC19 Control Plasmid (10-4 μg/μl)
5 µl
Instruction Manual
1
Champion™ Competent Cell Preparation Card
1
Storage
4°C for 12 months
Document
Champion™ E. coli Transformation Kit provides an easy method for rapid preparation of chemically competent cells with high transformation efficiency from fresh culture, overnight culture, or even directly from bacterial colonies on the plate. The competent cell preparation method eliminates the requirement of time-wasting wash step. In addition, preparation of competent cells from overnight culture or directly from bacterial colonies provides flexibility to cloning experiment. The resultant competent cells can be immediately used or stored at -70°C for one year. This kit includes a specialized SMO-Broth™ medium and a unique Champion™ CC Buffer for culturing and preparing competent cells efficiently. Following the simple and quick competent cell preparation protocol from fresh culture, the transformation efficiency is typically ranged from 108–109 cfu/μg transformants/μg of pUC19 plasmid DNA, but varies depending on the E. coli strains. The resultant competent cells can be further transformed using time-saving transformation protocol, eliminating the requirement of heat-shock and recovery steps.
1. Brushless frequency motor, in great torque, free maintenance, no power pollution, quick in speed up and down.
2. 7” LCD Touch screen display which indicates the program, rotor No, speed, time, RCF and temperature.
3. CFC-free refrigerant, pre-cooling function ,double cycle cooling, cold and hot alternating easily, free environment pollution and precise in temperature control.
4. Microprocessor control , 100 programs in store, 10 kinds of acceleration and deceleration for your choice.
5. Over speed and imbalance protection.
6. Speed, RCF, time, temperature can be edited during running.
7. High quality steel centrifuge body , stainless steel chamber, built-in stainless steel explosion-proof protect sleeve, 3 tiers protection, safe and reliable.
8. Automatic door open & close, as well as anti-pinch function (Optional).
CDL7M Technical Parameter：
Max. Speed
7000rpm
Max. RCF
11650×g
Max. Capacity
6×2400ml (12*500ml blood bag）
Time Range
1~99h59min59s
RPM/RCF Convert
Yes
Noise (dB)
≤ 65
Temperature
-20 ~ 40℃
Acc/Dec
10 Kinds
Speed Accuracy
±20r/min
Temperature Accuracy
±1℃
Voltage(V/Hz)
AC 220V/380V 50HZ/60HZ
Size (W x D x Hmm)
940×890×1000mm
Net Weight(Kg)
570KG
Certificates
CE,ISO & Calibration report are available
Special rotor for blood bag separation
Order No.
Rotor No.
Max Speed (rpm)
Max Volume(ml)
Max. RCF（×g）
05202
Swing Rotor
4000
6×2x1000ml
5210
Document
CDL7M touch screen with special programs for blood bag separation, can get good performance for platelet, red cell, all parts separation of blood. 6x2x1000ml bucket suitable for blood bag separation. Also with automatical lid lock open and close function, also wind shield can open along with door open.
Model:
CDL7M
