For cultivating of bacteria.And sterility test of drugs and biological products.
Principle:
Tryptone, peptone and yeast extract multivalent powder provides a nitrogen source, vitamins, and growth factors; sodium chloride to maintain osmotic balance; glucose carbon source; dipotassium hydrogen phosphate as a buffering agent.
Formulation(per liter):
Pancreatic Digest of Casein 17g
Papaic Digest of Soybean 3g
Sodium chloride 5g
Dipotassium hydrogen phosphate 2.5g
Glucose Monohydrate 2.5g
Final pH 7.3±0.2
How to use:
1.Suspend 30g in 1L of distilled water , stirring heated to boiling to completely dissolve ,autoclave at 121 for 15 minutes.
2.Diluted and treated samples.
Storage: Keep container tightly closed, store in a cool, dry place, away from bright light. Storage period of 3 years.
Other Products
IVD4173 HiPure Viral DNA/RNA Kit
Product Info
Document
Product Info
Introduction
This kit is used for extracting total viral nucleic acid from non-cell/low cell content biological samples such as body fluid, serum, plasma, immersion solution, tissue homogenate supernatant, culture supernatant, etc., the extracted products can be used for clinical in vitro detection.
Details
Specifications
Features
Specifications
Main Functions
Extract viral RNA/DNA from 200μl plasma/serum samples
Applications
RT-PCR，PCR，NGS
Products
Viral total nucleic acid, body cell total nucleic acid, negative bacterial DNA
Purification method
Mini spin column
Purification technology
Silica technology
Process method
Manual (centrifugation or vacuum)
Sample type
Whole blood, plasma, serum, soaking solution and tissue homogenate supernatant
Sample amount
200μl
Yield
2-10μg
Elution volume
≥30μl
Time per run
≤30 minutes
Liquid carrying volume per column
800μl
Binding yield of column
100μg
Principle
Hipure silica gel column is based on glass fiber filter membrane with high binding force. Under the condition of high concentration of ionizing agent (such as guanidine hydrochloride or guanidine isothiocyanate), the filter membrane can adsorb nucleic acid through hydrogen bond and electrostatic, while protein and other impurities are not adsorbed and removed. The filter membrane adsorbed with nucleic acid is washed to remove the residual protein and salt. Finally, the nucleic acid adsorbed on the filter membrane can be washed out with low salt buffer (such as buffer TE) or water. The obtained nucleic acid has high purity and can be directly used in various downstream experiments.
Advantages
Fast – several samples can be extracted in 20 minutes by column method
High quality – high purity total RNA can be directly used in various sensitive downstream applications
Safe – no phenol chloroform extraction required
Sensitive – DNA/RNA can be recovered at the level of PG
High yield – carrier RNA contained in the product maximize the recovery of trace nucleic acid
Kit Contents
Contents
IVD4173
Purification Times
100 Preps
HiPure Viral Mini Column
100
2ml Collection Tubes
200
PK/Carrier RNA
50 mg
Protease Dissolve Buffer
5 ml
Buffer AL
30 ml
Buffer MW1
44 ml
Buffer MW2
50 ml
RNase Free Water
15 ml
Storage and Stability
This kit is shipped and stored at room temperature and is valid for 12 months.
Experiment Data
Document
This kit is used for extracting total viral nucleic acid from non-cell/low cell content biological samples such as body fluid, serum, plasma, immersion solution, tissue homogenate supernatant, culture supernatant, etc., the extracted products can be used for clinical in vitro detection.
Reagent technology of protein/enzyme system: freeze-dried powder, freeze-dried microspheres
Document
This kit is based on a rapid nucleic acid amplification technology at room temperature and constant temperature: at room temperature and constant temperature (generally 39ºC~42ºC), with the help of auxiliary proteins and single-strand binding proteins,the recombinase and primers form a complex; Source search and combine the target homology domain, at this time, a D-loop region is formed at the homology position and strand exchange begins;along with the dissociation of the recombinase from the complex,the polymerase also binds to the 3′ end of the primer and begins chain extension.It is suitable for laboratory-level DNA amplification and DNA amplification for other detection purposes.
Usages: For determination of total bacterial count.
Principle: Tryptone provide carbon and nitrogen; yeast extract powder provides B vitamins; glucose to provide energy, agar is the solidifying agent.
Formulation（per liter）: Tryptone 5.0g Yeast extract powder 2.5g Glucose 1.0g Agar 15.0g Final pH 7.0 ± 0.2
How to use： 1.Suspend 23.5g in 1 L of distilled water , stirring heated to boiling until completely dissolved, dispensing flask, 121 ℃ autoclave for 15min. 2.Diluted and treated samples.
Quality control：
Item
The name and number of strain
Growth
Colony
1
E.Coli ATCC8739
Good
white
2
Staphylococcus aureus ATCC6538
Good
yellow
Storage: Keep container tightly closed, store in a cool, dry place, away from bright light. Storage period of 3 years.
