Intended Use For enriching bile-tolerant Gram-negative bacteria in the microbiological examination of pharmaceutical products. Principle and Interpretation Pancreatic digest of gelatin pro……
Detail
Introduction
Intended Use
For enriching bile-tolerant Gram-negative bacteria in the microbiological examination of pharmaceutical products.
Principle and Interpretation
Pancreatic digest of gelatin provide protein, vitamins and amino acids; glucose carbon source; disodium hydrogen phosphate and potassium dihydrogen phosphate as a buffer; ox bile and brilliant green as selective antibacterial agent, inhibiting the growth of non-Enterobacteriaceae.
Formulation
Ingredients
/liter
Pancreatic digest of gelatin
10g
Glucose monohydrate
5g
Dehydrated ox bile
20g
Potassium dihydrogen phosphate
2g
Disodium hydrogen phosphate dihydrate
8g
Brilliant green
15mg
pH7.2±0.2 at 25°C
Preparation
Suspend 45g in 1L of distilled water,stir until completely dissolved and dispense 100 mL into test tubes , heat at 100 °C for 30 min in a waterbath or flowing steam.
Quality Control
The following quality control strains were inoculated and cultured at 30-35℃ for 24h-48h. The results are as follows:
Quality control strains
Inoculum (CFU)
Growth
Escherichia coli ATCC8739
< 100
Good growth
Pseudomonas aeruginosa ATCC9027
Staphylococcus aureus ATCC6538
> 100
Inhibited
Storage and Shelf Life
2-30℃，Keep container tightly closed, avoid direct sunlight.
Use before expiry date on the label.
Precautions
1. When weighing the dehydrated medium, please wear masks to avoid causing respiratory system discomfort
2. Keep container tightly closed after using to prevent clumping.
Waste Disposal
Microbiological contamination was disposed by autoclaving at 121°C for
30 minutes.
Other Products
Staphylococcus Aureus PCR Pathogen Detection Kit For Isothermal Fluorescence Detector
Product Info
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Product Info
Product Description
Product Description:
Staphylococcus Aureus PCR Detection Kit for Isothermal Fluorescence Detector
Amp-future Bio’s Pathogen Detection Kit is a revolutionary breakthrough in infectious disease testing. It provides medical professionals with a comprehensive and rapid solution for the diagnosis of infectious diseases. Our Pathogen Detection Kit can detect a wide variety of infectious agents, including bacteria, viruses, parasites and fungi, within 5-20 minutes. It is designed for use with a variety of sample types, including blood, saliva, and other bodily fluids. Our kit features a powerful molecular diagnostic system that is tailored to detect pathogens quickly and accurately. This system is designed to be used by medical professionals and offers superior accuracy and sensitivity. With our Pathogen Detection Kit, medical professionals can quickly diagnose and treat infectious diseases with confidence. Our kit is also designed for easy storage at -20°C, ensuring reliable results.
Amp-future bio, a leading Chinese manufacturer of detection kits, is proud to introduce its new Pathogen Detection Kit. This innovative swab test kit is specially designed for rapid detection of influenza and other infectious diseases, delivering results in as little as 5-20 minutes. Featuring a storage temperature of -20ºC, this test kit is highly reliable and easy to use. It is ideal for hospitals, medical centers, and other healthcare facilities to quickly and accurately detect pathogens. As a trusted name in the industry, Amp-future bio guarantees the highest quality and safety when it comes to its Pathogen Detection Kit, making it the perfect choice for all your rapid test kits and infectious disease test kits needs.
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Pathogen Detection Kit technical support and services are offered to customers to ensure optimal performance and usability of the product. Technical support is available through our customer service hotline as well as our online customer portal. Our technical support team is available to answer any questions and provide guidance for product installation, troubleshooting, and maintenance. Additionally, our team provides software updates and upgrades, as well as access to our library of technical documents and resources. We also offer training and education services for customers who wish to better understand the product and its capabilities.
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To ensure that our Pathogen Detection Kit arrives to you in perfect condition, we take special care in packaging and shipping.
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Document
Staphylococcus Aureus PCR Detection Kit for Isothermal Fluorescence Detector
Amp-future Bio’s Pathogen Detection Kit is a revolutionary breakthrough in infectious disease testing. It provides medical professionals with a comprehensive and rapid solution for the diagnosis of infectious diseases. Our Pathogen Detection Kit can detect a wide variety of infectious agents, including bacteria, viruses, parasites and fungi, within 5-20 minutes. It is designed for use with a variety of sample types, including blood, saliva, and other bodily fluids. Our kit features a powerful molecular diagnostic system that is tailored to detect pathogens quickly and accurately. This system is designed to be used by medical professionals and offers superior accuracy and sensitivity. With our Pathogen Detection Kit, medical professionals can quickly diagnose and treat infectious diseases with confidence. Our kit is also designed for easy storage at -20°C, ensuring reliable results.
Short term stability: 2-8oC, Long term stability: See individual component labels
Stability:
> 2 years under recommended storage conditions
Analyte:
Digestible Starch, Resistant Starch, Total Starch
Assay Format:
Spectrophotometer
Detection Method:
Absorbance
Wavelength (nm):
510
Signal Response:
Increase
Linear Range:
4 to 100 μg of D-glucose per assay
Limit of Detection:
3.1 g/100 g
Reaction Time (min):
~ 360 min
Application examples:
Plant materials, starch samples and other materials.
The Digestible and Resistant Starch Assay Kit (K-DSTRS) for the determination of digestible, resistant and total starch in starch samples, plant and other materials.
This method is based on the research of Englyst et al. (Ref) with some modifications. Digestion is performed using saturating levels of pancreatic α-amylase (PAA) and amyloglucosidase (AMG), but in stirred containers rather than shaken tubes, to simplify sample removal.
In line with Englyst definitions:
Rapidly digestible starch (RDS) is that starch which is digested within 20 min.
Slowly digestible starch (SDS) is that starch which is digested between 20 and 120 min.
A new term, ‘Total digestible starch (TDS)’ is introduced (and measured) to cover all starch that is digested within 4 h (the average time of residence of food in the human small intestine).
Resistant starch (RS) then, is that starch which is not digested within 4 h.
The incubation conditions parallel those used in AOAC Method 2017.16, a new, rapid integrated procedure for the measurement of total dietary fiber (Megazyme method K-RINTDF). This method is physiologically based and designed to fit the definition of DF announced by Codex Alimentarius in 2009.
The Digestible and Resistant Starch Assay Kit (K-DSTRS) for the determination of digestible, resistant and total starch in starch samples, plant and other materials.
The 16S ribosomal RNA (rRNA) plays a crucial role in bacterial and archaeal ribosomes. This sequence is Highly conserved across bacteria and archaea, it contains variable regions useful for species differentiation and is part of the 30S subunit of prokaryotic ribosomes. 16s rRNA Binds to the Shine-Dalgarno sequence and contributes to the subunit’s structure. Acts as a scaffold for ribosomal proteins.
Because the 16s rRNA sequence is ubiquitous in bacteria and archaea, it can be used to identify a wide diversity of microbes within a single sample and single workflow.
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This kit is sufficient for 150 reactions:
For characterizing cyanobacteria in environmental samples
Use in combination with Attogene Algae DNA isolation kit
Universal 16s PCR primers
Perfect for Environmental DNA (eDNA) Characterization
