For cultivations of yeasts , moulds and acidophilic bacteria.
Principle:
Peptone and yeast extract powder provides carbon and nitrogen sources and trace elements; lactose are fermentable sugars; sodium chloride maintains osmotic equilibrium; the 3rd bile salts and crystal violet inhibiting gram-positive bacteria, especially against Gram positive bacteria and fecal streptococci; neutral red as pH indicator.
Formulation(per liter):
Peptone 5g
Tryptone 5g
Glucose 20g
Final pH 5.7 ± 0.1
How to use:
1.Suspend 30g in 1 L of distilled water , stirring heated to boiling until completely dissolved, dispensing flask, 121 autoclave for 15min.
2.Diluted and treated samples.
Quality control:
Item
The name and number of strain
Growth
Colony Color
1
Aspergillus niger ATCC16404
Good
Spore surface growth
2
Candida albicans ATCC10231
Good
Cloudy broth
3
Escherichia coli ATCC25922
Inhibition
—
Storage: Keep container tightly closed, store in a cool, dry place, away from bright light. Storage period of 3 years.
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Attogene is offering this highly robust test to detect SEB by lateral flow in diverse liquid sample types.
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Isolate all sizes of RNA, including microRNA, irrespective of size or GC content, without bias.
Versatile cell culture media input volumes
No phenol extractions, Proteinase K treatment, nor carrier RNA required
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No precipitation reagents nor overnight incubation required
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