HCM145 Modified MSRV Agar Base

Overview

• Versatile urine input ranges
• No phenol extractions
• Concentrate circulating RNA and exosomal RNA into flexible elution volumes
• Purified RNA is suitable for a variety of downstream applications, including Small RNA Sequencing. Find out more information on Norgen’s NGS Services
• EXTRAClean resin separation matrix provides highly purified RNA.

Norgen’s EXTRAClean Urine Cell-Free Circulating RNA Purification Kits provide a fast, reliable, reproducible, and simple procedure for isolating circulating RNA and exosomal RNA from various urine inputs ranging from 250 μL and up to 30 mL. The purified RNA is of the highest integrity, and can be used in a number of downstream applications including real-time PCR, reverse transcription PCR, Northern blotting, RNase protection and primer extensions, and expression array assays.

EXTRAClean Cell-Free Circulating RNA Purification Mini Kit:
For sample volumes ranging from 250 μL to 2 mL

EXTRAClean Cell-Free Circulating RNA Purification Midi Kit:
For sample volumes ranging from 2mL to 10mL. The first column will handle the large volume input of urine that is followed by a concentration on a mini column for a final elution of 50 μL to 100 μL

EXTRAClean Cell-Free Circulating RNA Purification Maxi Kit:
For sample volumes ranging from 10 mL to 30 mL. The first column will handle the large volume input of urine that is followed by a concentration on a mini column for a final elution of 50 μL to 100 μL.

Details

Supporting Data

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Kit Specifications
Sample Type	Fresh or frozen urine
Sample Volume Range	2 to 10 μL
Minimum Elution Volume	50 μL
Maximum Elution Volume	100 μL
Time to Complete 10 Purifications	40–45 minutes
Size of RNA Purified	All sizes, including miRNA and small RNA (< 200 nt)
Average Yields ¥	Variable depending on specimen

^¥Please check page 6 of the protocol for average urine yields and common RNA quantification methods.

Storage Conditions and Product Stability

All buffers should be kept tightly sealed and stored at room temperature. These kits are stable for 2 years after the date of shipment. It is recommended to warm Lysis Buffer A for 20 minutes at 60°C if any salt precipitation is observed.

Component	Mini (Cat. 72900)	Midi (Cat. 72910)	Maxi (Cat. 72920)
Binding Solution K	25 mL	75 mL	1 x 75 mL 1 x 25 mL
Lysis Buffer A	30 mL	20 mL	20 mL
Wash Solution A	18 mL	18 mL	18 mL
Elution Solution A	6 mL	6 mL	6 mL
Collection Tubes	50	20	10
Elution Tubes (1.7 mL)	50	20	10
EXTRAClean Mini Spin Columns	50	20	10
EXTRAClean Midi Spin Columns	–	20	–
EXTRAClean Maxi Spin Columns	–	–	10
Product Insert	1	1	1