HCM145 Modified MSRV Agar Base

K-PullG6

SKU: 700004329

100/200 assays per kit

Content:	100 / 200 assays per kit
Shipping Temperature:	Ambient
Storage Temperature:	Short term stability: 2-8oC, Long term stability: See individual component labels
Stability:	> 2 years under recommended storage conditions

Analyte:	Pullulanase/Limit-Dextrinase
Assay Format:	Spectrophotometer
Detection Method:	Absorbance
Wavelength (nm):	400
Signal Response:	Increase
Limit of Detection:	0.18 U/mL for pullulanase preparations (50-fold dilution) 0.01 U/g for limit dextrinase in milled malt
Reproducibility (%):	~ 3%
Total Assay Time:	~ 10 min (Pullanase), ~ 30 min (Limit-Dextrinase)
Application examples:	Assay of microbial pullulanase preparations. Measurement of limit-dextrinase in malt extracts.
Method recognition:	Novel method

PullG6 assay for the measurement of pullulanase employs a water soluble defined substrate, namely 4,6-O-benzylidene-4-nitrophenyl-6³-α-D-maltotriosyl-maltotriose (BPNPG3G3), coupled with the ancillary enzymes α-glucosidase and β-glucosidase. Upon hydrolysis of the substrate at the 1,6-α-linkage by pullulanase or limit-dextrinase, the released 4-nitrophenyl-β-maltotrioside is immediately hydrolysed to glucose and 4-nitrophenol by the concerted action of the α-glucosidase and β-glucosidase enzymes in the reagent mixture. The reaction is terminated and phenolate ions are developed by addition of dilute alkali. The absorbance is read at 400 nm and the value obtained correlates directly with pullulanase activity.

Explore more of our assay kit products for enzyme activity measurement.

Advantages

• High sensitivity 
• Suitable for manual and auto-analyser formats 
• No transglycosylation interference 
• Very cost effective 
• All reagents stable for > 1 year after preparation 
• Very specific
• Simple format
• Standard included

m-PEG4-DBCO is a click chemistry reagents which can enable so called copper-free Click Chemistry with azide to form a stable triazole. Reagent grade, for research purpose. Please contact us for GMP-grade inquiries.

m-PEG4-DBCO is a click chemistry reagents which can enable so called copper-free Click Chemistry with azide to form a stable triazole. Reagent grade, for research purpose. Please contact us for GMP-grade inquiries.

Overview

• Detection kits for Clostridium difficile
• CE-IVD marked version available for in vitro diagnostic use
• Available in TaqMan format for analysis

Clostridium difficile is rod-shaped, gram positive bacterium. It is the main causal agent of antibiotic-associated diarrhea and pseudomembranous colitis. The colonization of intestines by C. difficile is usually associated with the elimination of natural intestinal flora as a result of antibiotic application and is frequently reported in health care centers. While C. difficile infection could be severe and life-threatening, particularly among the elderly, many patients are asymptomatic making diagnosis challenging during outbreaks. The tradition method of detecting C. difficile infection is by cytotoxicity test of the toxin produced by the bacterium, but such protocols usually require extensive time before conclusion can be made.

Clostridium difficile TaqMan PCR Kit, 100 reactions

• Ready to use format, including Master Mix for the target and PCR control to monitor for PCR inhibition and validate the quality
• Specific Primer and Probe mix for the pathogen/virus/viroid of interest
• Primer and Probe mix
• Positive and negative control to confirm the integrity of the kit reagents

Clostridium difficile TaqMan PCR Probe/Primer Set and Controls, 100 reactions

• Specific Primer/Probe mix and Positive Control for the pathogen/virus/viroid of interest
• Nuclease-free water
• Can be used together with Norgen’s PCR Master Mix (#28007) or customer supplied master mix

For research use only and NOT intended for in vitro diagnostics.

Details

Supporting Data

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Storage Conditions and Product Stability
All kit components can be stored for 2 years after the date of production without showing any reduction in performance.

All kit components should be stored at -20°C upon arrival. Repeated thawing and freezing (> 2 x) of the Master Mix and Positive Control should be avoided, as this may affect the performance of the assay. If the reagents are to be used only intermittently, they should be frozen in aliquots.

Component	Cat. TM37150 (100 preps)	Cat. TM37110 (100 preps)
MDx TaqMan 2X PCR Master Mix	2 x 700 μL	–
Clostridium difficile Primer & Probe Mix	280 μL	280 μL
Clostridium difficile Positive Control	150 μL	150 μL
Nuclease-Free Water (Negative Control)	1.25 mL	1.25 mL
Product Insert	1	1