
Introduction
Discover Huankai’s Modified MSRV Agar Base, designed for the selective detection and isolation of motile Salmonella species, ensuring reliable food and environmental testing.
Discover Huankai’s Modified MSRV Agar Base, designed for the selective detection and isolation of motile Salmonella species, ensuring reliable food and environmental testing.
Discover Huankai’s Modified MSRV Agar Base, designed for the selective detection and isolation of motile Salmonella species, ensuring reliable food and environmental testing.
Usages:
Determination of coliform and fecal coliform for multiple tube fermentation.
Principle:
Tryptone provide carbon and nitrogen sources to meet the needs of bacterial growth; sodium chloride osmotic pressure balance can be maintained; Lactose is a coliform fermentable sugars; potassium dihydrogen phosphate and dipotassium phosphate is a buffer; lauryl sodium can inhibit the growth of non-coliform bacteria.
Formulation (per liter):
Trypticase:20g
Sodium chloride: 5g
Lactose:5g
Potassium dihydrogen phosphate: 2.75g
Dipotassium hydrogen phosphate :2.75g
Sodium lauryl sulfate 0.1g
Final pH6.8 ± 0.2
How to use:
1. Suspend 35.6g of the product, adding 1 L of distilled or deionized water, heated to boiling stirring until completely dissolved, packed in a test tube with a small down tube, 121 ℃ autoclave 15min, leave to cool to room temperature, standby .
2.Sample handling and dilution.
3.Selected three consecutive dilution, each dilution was inoculated three LST broth tubes, each tube was inoculated 1mL.
4. Put the tubes in an incubator 36 ± 1 ℃ cultured for 48 ± 2h.
5. Observe the results. if all LST broth don’t pruduse gas, can be reported as negative for E. coli, if gas production then will have to make further confirmed by experiments.
Quality control:
Quality control strains were inoculated and culuture at 36 ± 1 ℃ for 24h ,results are as follows:
Bacterial Name Bacterial No. Growth Status Gassing
Escherichia coli ATCC25922 good +
Salmonella typhimurium CMCC (B) 50115 good —
Staphylococcus aureus ATCC6538 inhibited —
Storage: Store in a dark, cool and dry place, tighten the cap immediately after use. Storage period of three years.
Specifications: 250g/bottle
250g
Norgen’s NGS Normalization 96-Well Kit allows for high-throughput NGS library PCR amplicon clean-up and normalization of NGS library PCR product concentration. The clean-up removes all PCR by-products including primers, dimers, enzymes and unincorporated nucleotides during the library prep PCR step. Purification is based on 96-well column chromatography using Norgen’s proprietary resin as the separation matrix. The amount of Norgen’s matrix in each 96 well is optimized to have a limited binding capacity, thus each well can elute an equal concentration of PCR product. The process involves first adding 3 volumes of Buffer SK to the PCR product, and the mixture is then loaded onto the 96-Well Normalization Plate. The bound PCR amplicon is then washed with the provided Wash Solution A in order to remove any remaining impurities, and the purified PCR amplicon is eluted with Elution Buffer B. The purified and normalized library PCR amplicon can then be used in NGS workflows and other sequencing applications.
Figure 1 / 3
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Storage Conditions
All solutions should be kept tightly sealed and stored at room temperature. This kit is stable for 2 years after the date of shipment.
Component | Cat. 61900 (192 preps) |
---|---|
Buffer SK | 30 mL |
Wash Solution A | 2 x 38 mL |
Elution Buffer B | 30 mL |
96-Well Normalization Plate | 2 |
96-Well Collection Plate | 2 |
96-Well Elution Plate | 2 |
Adhesive Tape | 1 |
Product Insert | 1 |
K-GLUT
SKU: 700004301
60 assays (manual) / 600 assays (microplate) / 700 assays (auto-analyser)
Content: | 60 assays (manual) / 600 assays (microplate) / 700 assays (auto-analyser) |
Shipping Temperature: | Ambient |
Storage Temperature: | Short term stability: 2-8oC, Long term stability: See individual component labels |
Stability: | > 1 year under recommended storage conditions |
Analyte: | L-Glutamic Acid, MSG |
Assay Format: | Spectrophotometer, Microplate, Auto-analyser |
Detection Method: | Absorbance |
Wavelength (nm): | 492 |
Signal Response: | Increase |
Linear Range: | 0.4 to 20 µg of L-glutamic acid per assay |
Limit of Detection: | 0.21 mg/L |
Reaction Time (min): | ~ 8 min |
Application examples: | Fruit and vegetables (e.g. tomato), processed fruit and vegetables (e.g. tomato puree / juice, ketchup, soy sauce), condiments, processed meat products (e.g. extracts, bouillon and sausages), soup, pharmaceuticals and other materials (e.g. biological cultures, samples, etc.). |
Method recognition: | Methods based on this principle have been accepted by ISO, GOST and NMKL |
The L-Glutamic Acid test kit is a simple, reliable, rapid and accurate method for the measurement and analysis of L-glutamate (MSG) in foodstuffs.
Note for Content: The number of manual tests per kit can be doubled if all volumes are halved. This can be readily accommodated using the MegaQuantTM Wave Spectrophotometer (D-MQWAVE).
Explore more organic acid test kits.
Advantages
The L-Glutamic Acid test kit is a simple, reliable, rapid and accurate method for the measurement and analysis of L-glutamate (MSG) in foodstuffs.
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