High Purity DNA Purification Kit Fast Extract Isolate Nucleic Acids
Facebook
X
Pinterest
Email
Detail
High Purity DNA Purification Kit
,
12 Months DNA Purification Kit
,
Nucleic Acid dna isolation kit
Product Description
Rapid Nucleic Acid Release Reagent (DNA) – Type II
High Purity DNA Purification Kit Fast Extract Isolate Nucleic Acids
Product parameters:
Reagent component (WLD8201-ES,2Tubes/box)
Component
Specification
Quantity
LD-1
800μL＝0.8mL
1 Tube
LD-2
200μL＝1.2mL
1 Tube
Product features and advantages:
Product features
Simplicity
The operation is simple, and the DNA template for amplification can be obtained in just one step (conventional 5min)
Security
Non-toxic environmental protection, room temperature transport and storage
Universality
The lysate can be used for isothermal nucleic acid amplification
Product application:
It is used for pre-processing and nucleic acid release of swab samples and plant tissue samples, rapidly cracking all kinds of common samples without purification; The sample lysate can be directly used as a template for amplification reaction.
Tips: Its reverse combined with our constant temperature amplification reagent for nucleic acid detection.
Sample processing type
Liquid sample
Samples of plants or tissues
Serum, blood, saliva, ascites and other samples, swab leach solution
Brain, muscle, viscera, leaves, fruit and other samples, take liquid supernatant
Other Products
024020P1 Mannitol Salt Agar
Product Info
Document
Product Info
Introduction
Usage:
For selective isolation and culture of Staphylococcus aureus.
Principle:
Peptone and beef extract powder provides carbon, nitrogen, vitamins and minerals; D- mannitol to fermentable sugars; higher levels of sodium chloride to provide a higher osmotic pressure, suppress most non-staphylococcal microorganisms ; phenolsulfonphthalein as pH indicator; agar is medium coagulant. Typical pathogenic staphylococci (coagulase positive) D- mannitol produce acid fermentation and produce yellow colonies with a yellow halo, typically non-pathogenic Staphylococcus unfermented D- mannitol to form red colonies.
Formulation(per liter): Pancreatic digest of casein 5.0g Pancreatic digest of animal tissue 5.0g Beef Extract 1.0g Sodium Chloride 75.0g Mannitol 10.0g Phenol Red 0.025g Agar 15.0g Final PH 7.4±0.2
How to use： 1.Suspend 111g in 1L of distilled water , stirring heated to boiling to completely dissolve ,autoclave at 121℃ for 15 minutes. 2.Diluted and treated samples.
Quality control：
Item
The name and number of strain
Growth
Colony Color
1
Staphylococcus aureus CMCC (B) 26003
Good
Golden yellow
2
Staphylococcus epidermidis CMCC (B) 26069
Good
Red
3
Escherichia coli CMCC (B) 44102
Inhibition
—
Storage: Keep container tightly closed, store in a cool, dry place, away from bright light. Storage period of 3 years.
Dietary fiber can generally be described as the carbohydrate content of food that is not digested in the human small intestine. It passes into the large intestine where it is partially or fully fermented. These characteristics of dietary fiber are associated with its numerous well documented health benefits.
Dietary Fiber is a mixture of complex organic substances, including hydrophilic compounds, such as soluble and insoluble polysaccharides and non-digestable oligosaccharides, as well as a range of non-swellable, more or less hydrophobic, compounds such as cutins, suberins and lignins. The procedures for the determination and analysis of total dietary fiber as outlined in our assay protocol are based on the methods of Lee et al.1 and Prosky et al.2,3 (AOAC 991.43, AOAC 985.29, AACC 32-07.01 and AACC 32-05.01). However, the enzymes in the Megazyme Total Dietary Fiber Kit can also be used in other dietary fiber analytical methods such as AACC Method 32-21.01 and AACC Method 32-06.01.
1. Association of Official Analytical Chemists. (1985). Official Methods of Analysis, 14th ed., 1st suppl. Secs. 43, A14-43, A20, p.399. 2. Association of Official Analytical Chemists. (1986). Changes in methods. J. Assoc. Off. Anal. Chem., 69, 370. 3. Association of Official Analytical Chemists. (1987). Changes in methods. J. Assoc. Off. Anal. Chem., 70, 393.
See General Referee Reports: Journal of AOAC INTERNATIONAL, Vol. 81, No. 1, 1998.
Two separate methods are described in the associated assay protocol:
METHOD 1: DETERMINATION OF TOTAL, SOLUBLE AND INSOLUBLE DIETARY FIBER Based on AOAC Method 991.43 “Total, Soluble, and Insoluble Dietary Fiber in Foods” (First Action 1991) and AACC Method 32-07.01 “Determination of Soluble, Insoluble, and Total Dietary Fiber in Foods and Food Products” (Final Approval 10-16-91).
METHOD 2: DETERMINATION OF TOTAL DIETARY FIBER Based on AACC method 32-05.01 and AOAC Method 985.29.
Note that a letter of endorsement from the original method developer, Dr. Leon Prosky, is included in the Documents Tab.
Peelable heat sealing foil which is suitable for low temperature storage, high temperature uses and PCR.
Heat sealing offers a 100% effective method of plate sealing, for complete seal integrity, as well as being quick and cost effective
Our PeelASeal Foil is a laminate seal compatible with polypropylene plates
It can be removed from polypropylene plates by peeling, even with a plate which has been removed directly from -80 °C storage
PeelASeal Foil forms a complete seal to a plate enabling very low temperature uses, including very low temperature storage, and high temperature uses, such as PCR (when used with a pressurized heated lid)
The seal demonstrates moderate solvent resistance and can be utilized for short term compound storage at room temperature
The seal is available as sheets, for use with manual and semi-automated sealers, such as our HeatASeal 500 Sealing Machine
Also available in multiple roll formats compatible with specified automated heat sealers, such as our Wasp or Chameleon XT
Document
Peelable heat sealing foil which is suitable for low temperature storage, high temperature uses and PCR.
