HIT – Heparin-Induced Thrombocytopenia Test (20)

K-FRUC

SKU: 700004285

100 assays per kit

Content:	100 assays per kit
Shipping Temperature:	Ambient
Storage Temperature:	Short term stability: 2-8oC, Long term stability: See individual component labels
Stability:	> 2 years under recommended storage conditions

Analyte:	Fructan
Assay Format:	Spectrophotometer
Detection Method:	Absorbance
Wavelength (nm):	410
Signal Response:	Increase
Linear Range:	2.3 to 55 µg of D-fructose or D-glucose per assay
Limit of Detection:	0.16 g/100 g
Total Assay Time:	~ 90 min
Application examples:	Flours, infant formula, animal feed, pet foods, plant materials (e.g. onion), food products and other materials
Method recognition:	AACC Method 32-32.01, AOAC Method 999.03, AOAC Method 2016.14, AOAC Method 2018.07 and CODEX Method Type III

The Fructan Assay Kit is suitable for the specific measurement of fructan in plant extracts, animal feed and food products containing starch, sucrose and other sugars. It is used in three validated methods for the determination of fructan: AOAC method 999.03 (foods), AOAC method 2018.07 (Animal Feed) and AOAC method 2016.14 (infant formula and adult nutritionals).

New, improved procedure.

In the most recent development, a recombinant endo-levanase has been incorporated into the fructanase mixture, extending the use of the method to the measurement of levan-type fructans as are present in grasses such as timothy, cocksfoot, ryegrass and red fescue.

The method described in this booklet employs ultra-pure, recombinant enzymes and specifically measures fructans including inulin-type fructans from chicory, dahlia, jerusalem artichoke; highly branched fructans from onion and wheat stems and leaves; and levan-type fructans from pasture grasses such as timothy grass. The enzymes employed are completely devoid of contaminating enzymes active on β-glucan or gluco-oligosaccharides.

Browse our full range of polysaccharide assay kits.

Validation of Methods

Advantages

• Very cost effective 
• All kit reagents stable for > 2 years after preparation 
• Unaffected by high sucrose / reducing sugar concentrations  
• Fructan kits are only available from Megazyme 
• Simple format 
• Mega-Calc™ software tool is available from our website for hassle-free raw data processing 
• Standard included

The Fructan Assay Kit is suitable for the specific measurement of fructan in plant extracts, animal feed and food products containing starch, sucrose and other sugars. It is used in three validated methods for the determination of fructan: AOAC method 999.03 (foods), AOAC method 2018.07 (Animal Feed) and AOAC method 2016.14 (infant formula and adult nutritionals).

TGL20 Technical Parameters
Max Speed	21000r/min	Max Volume	6×100ml
Timer	1~99h59min	Dimension	570×620×380mm
Speed Accuracy	±20r/min	Max RCF	30910×g
Noise	≤58dBA	Net Weight	84KG
Power supply	AC 220V, 50HZ 10A	Temperature Range	-20℃~40℃
Temperature Accuracy	±1℃

Matched Rotors for TGL20
Order no	Rotor	Max Speed(r/min)	Volume(ml)	Max RCF(*g)
G20-1	Fixed Rotor	16000	4×8PCR	15760
G20-2	Fixed Rotor	15000	6×8PCR	21420
G20-3	Fixed Rotor	16000	8×8PCR	17480
G20-4	Fixed Rotor	15000	12×8PCR	22930
G20-5	Fixed Rotor	21000	12×1.5/2ml	30910
G20-6	Fixed Rotor	15000	40×0.5ml	22920
G20-7	Fixed Rotor	17000	24×1.5/2ml	26460
G20-8	Fixed Rotor	13500	30×1.5/2ml	19340
G20-9	Fixed Rotor	13000	48×1.5/2ml	17930
G20-10	Fixed Rotor	16000	16×5ml	22020
G20-11	Fixed Rotor	16000	6×10ml	21500
G20-12	Fixed Rotor	15000	12×10ml	22680
G20-13	Fixed Rotor	16000	12×7ml	21380
G20-14	Fixed Rotor	13000	16×10ml	19490
G20-15	Fixed Rotor	10000	12×15ml	11840
G20-16	Fixed Rotor	13000	8×15ml(falcon)	17790
G20-17	Fixed Rotor	5000	24×15ml	3500
G20-18	Fixed Rotor	15000	8×20ml	22680
G20-19	Fixed Rotor	5000	30×15ml	3830
G20-20	Fixed Rotor	14000	6×30ml	19060
G20-21	Fixed Rotor	13000	6×50ml(falcon)	18840
G20-22	Fixed Rotor	13000	6×50ml(round)	18730
G20-23	Fixed Rotor	13000	4×85ml	18940
G20-24	Fixed Rotor	12000	6×70ml	15570
G20-25	Fixed Rotor	12000	4×100ml	14850
G20-26	Fixed Rotor	12000	6×100ml	16390
G20-27	Fixed Rotor	12000	24 pieces capillary vessel	15800
G20-28	Fixed Rotor	18000	30×0.5ml	26660
G20-29	Swing Rotor	13000	4×5ml	14960
G20-30	Vertical Rotor	16000	16×5ml	16540
G20-31	Vertical Rotor	14000	8×30ml	16450
G20-32	Microplate Rotor	4000	2×3×48（well）	2300

Features:
1. Digital display indicates the speed,time,temperature and RCF.
2. Brushless DC motor in great torque, free maintenance,no powder pollution.quick in speed up and down.
3. There are many kinds of rotors for choice.
4. Automatically electric lid lock,super speed,over temperature protection and imbalance protection.
5. The centrifuge body is made of high-quality steel,safe and reliable.

Overview

• Detection kits for Cryptococcus neoformans
• Available in TaqMan format for analysis

Cryptococcus neoformans is an encapsulated yeast. Infection with C. neoformans is known as cryptococcosis and is the cause of the most common life-threatening meningitis in patients with weakened immune systems, particularly in advanced HIV/AIDS. C. neoformans is commonly found in soil throughout the world. Human infection of C. neoformans occurs via inhalation of aerosolized spores. From the lungs, C. neoformans is spread hematogenously to the Central Nervous System (CNS), resulting in meningoencephalitis. Although the availability of antiretroviral therapy in the developed world has reduced the incidents of cryptococcosis, it is still a major problem in developing countries and is one of the leading causes of death in patients with HIV/AIDS. In fact, one of the major challenges in treating cryptococcosis is that many patients with cryptococcal CNS disease are asymptomatic in terms of cryptococcal pneumonia, making it difficult for early detection.

Cryptococcus neoformans TaqMan PCR Kit, 100 reactions

• Ready to use format, including Master Mix for the target and PCR control to monitor for PCR inhibition and validate the quality
• Specific Primer and Probe mix for the pathogen/virus/viroid of interest
• Primer and Probe mix
• Positive and negative control to confirm the integrity of the kit reagents

Cryptococcus neoformans TaqMan PCR Probe/Primer Set and Controls, 100 reactions

• Specific Primer/Probe mix and Positive Control for the pathogen/virus/viroid of interest
• Nuclease-free water
• Can be used together with Norgen’s PCR Master Mix (#28007) or customer supplied master mix

Details

Supporting Data

Figure 1 / 3

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Storage Conditions and Product Stability
All kit components can be stored for 2 years after the date of production without showing any reduction in performance.

All kit components should be stored at -20°C upon arrival. Repeated thawing and freezing (> 2 x) of the Master Mix and Positive Control should be avoided, as this may affect the performance of the assay. If the reagents are to be used only intermittently, they should be frozen in aliquots.

Component	Cat. TM42750 (100 preps)	Cat. TM42710 (100 preps)
MDx TaqMan 2X PCR Master Mix	2 x 700 μL	–
Cryptococcus neoformans Primer & Probe Mix	280 μL	280 μL
Cryptococcus neoformans Positive Control	150 μL	150 μL
Nuclease-Free Water (Negative Control)	1.25 mL	1.25 mL
Product Insert	1	1