Exceptional value for money
Rapid detection of all clinically relevant subtypes
Positive copy number standard curve for quantification
Highly specific detection profile
High priming efficiency
Broad dynamic detection range (>6 logs)
Sensitive to < 100 copies of target
Accurate controls to confirm findings
150 reactions
Nucleic acid testing (NAT) is the method of choice for detection and quantification of a wide range of micro organisms. Primerdesign manufactures and supplies high quality quantitative real-time PCR kits for the detection and simultaneous quantification of numerous significant pathogens . A copy number standard curve is provided for quantification and an the internal extraction template (DNA or RNA), controls for the quality of the nucleic acid extraction and eliminates false negative results.
The kit is designed with the broadest possible detection profile to ensure that all clinically relevant strains and subtypes are detected. Target sequences are selected by working with data from key opinion leaders in the field. Multiple sequence alignments and unprecedented real-time PCR expertise in design and validation ensure the best possible kit. Details of the target and priming specificity are included in the individual handbooks above.
Packaged, optimised and ready to use. Expect Better Data.
Other Products
32 X 0.2 mL PCR Strip Magnetic Separator
Product Info
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Product Info
Permagen’s 32-tube PCR Separation rack features all of the same aspects of our MSR812 rack above for easy protocol transition for labs wishing to scale up to four PCR strips at a time, or up to 32 individual PCR tubes.
Features include solid aluminum alloy design with hard coat finish for years of trouble-free use, rubber feet to help prevent slipping on work bench, less tippy than common plastic products, and fast separations using any magnetic beads
Permagen’s 32-tube PCR Separation rack features all of the same aspects of our MSR812 rack above for easy protocol transition for labs wishing to scale up to four PCR strips at a time, or up to 32 individual PCR tubes.
The 16S V1-V2 Library Preparation Kit for Illumina consists of the reagents and components required for library preparation of the 16S V1-V2 amplicon libraries to be used for next-generation sequencing on Illumina platforms. All molecular reagents including primers, enzyme mixes, indexes, and buffers are provided. Instructions for PCR clean up with the AMPure XP Magnetic Beads (supplied by customer) are also included for rapid purification of nucleic acid products generated at two steps of the workflow. The library prep workflow could be used for purified DNA inputs from different sources including stool, soil, water, saliva, plant, urine, skin swab, vaginal swab, cheek swab, nasal swab, plasma/serum, tongue swab, gum swab, and others.
The 16S V1-V2 Library Preparation Kit for Illumina has a streamlined procedure that reduces the handling time such that the library prep procedure can be completed in approximately 4 hours (see diagram below). Input DNA is first subjected to targeted PCR to amplify the V1-V2 region of the DNA encoding 16S rRNA. The post-PCR reaction is then cleaned up using AMPure XP beads. Dual index primers are then added using a limited-cycle PCR. The indexed amplicons flanked by 5′ and 3′ barcoded adaptors are then cleaned using AMPure XP beads. The libraries are then ready for quantification, pooling and sequencing.
Storage Conditions and Product Stability Norgen’s 16S V1-V2 Library Prep Kit for Illumina is shipped as one kit box (for the 24 prep kit) or two sub-component kits (for the 96 prep kit). All kits should be stored at -20°C upon arrival.
All kit components should remain stable for at least 1 year when stored at the specified storage conditions.
Advanced performance at ultra-low reaction volumes, delivering consistent, cost-effective PCR genotyping for high-throughput, miniaturised and automated workflows.
About
Step into the future of high-throughput PCR genotyping with PACE Nano! Backed by decades of expertise in high-throughput genotyping reagent manufacturing, our groundbreaking product is engineered specifically to excel in low and ultra-low volume reactions. Say goodbye to performance variability as PACE Nano Genotyping Master Mix and PACE Genotyping Assays raise the bar for reliability and performance in the field.
With superior cost-effectiveness and versatile compatibility, PACE Nano Genotyping Master Mix ensures consistent results across diverse platforms, samples, and markers, including 384- and 1536-well PCR, Array Tape™, SNPline™ and Gene Mathematica from HC Scientific, available from 3CR Bioscience, supporting reaction volumes as low as 0.8 µL. Join the revolution today and elevate your research with PACE Nano.
Cost-Effective: Produce more high-quality data faster and at a much lower cost compared to competitors, reducing reagent expenses without compromising on quality.
Optimised for Low and Ultra-Low Volume PCR Genotyping: PACE Nano Genotyping Master Mix is specifically tailored for enhanced performance in miniaturised PCR genotyping, for reaction volumes as low as 0.8 µL and for high-throughput and automated processing with accurate, reliable results.
Consistent Data Across Assays: PACE Nano reduces variability in assay performance, enhancing the robustness and efficiency of high throughput, miniaturised and automated workflows.
Backed by 20 Years of Expertise: Designed and manufactured with 20 years of experience in high-throughput genotyping reagents, ensuring reliability and quality.
Scalability: Our PACE® genotyping reagents are platform agnostic, so you can adapt to changing throughput requirements effortlessly, ensuring flexibility and efficiency at every stage of your genotyping workflow.
PACE Nano Global Product Support: Benefit from a stable supply chain and unparalleled global product support, tailored to your needs regardless of size or throughput. Supported by the highest quality-assurance processes, ensuring reliability and consistency.
REQUIRED COMPONENTS
qPCR machine or Thermocycler + Fluorescent plate reader
PCR plate or equivalent and appropriate optically clear seal
Template DNA
PCR-grade water
Genotyping assays
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qPCR machine or Thermocycler + Fluorescent plate reader
PCR plate or equivalent and appropriate optically clear seal
Template DNA
PCR-grade water
Genotyping assays
