Primer and probe mix (150 reactions)
Reverse Transcription, target specific primers (RNA genome viruses only)
Copy number standard curve (sufficient for multiple standard curves)
Internal extraction control – Read through VIC channel*
Endogenous control (150 tests)
RNAse/DNAse free water
*alternative fluorophores available on request
Nucleic acid testing (NAT) is the method of choice for detection and quantification of a wide range of micro organisms. Primerdesign manufactures and supplies high quality quantitative real-time PCR kits for the detection and simultaneous quantification of numerous significant pathogens . A copy number standard curve is provided for quantification and an the internal extraction template (DNA or RNA), controls for the quality of the nucleic acid extraction and eliminates false negative results.
The kit is designed with the broadest possible detection profile to ensure that all clinically relevant strains and subtypes are detected. Target sequences are selected by working with data from key opinion leaders in the field. Multiple sequence alignments and unprecedented real-time PCR expertise in design and validation ensure the best possible kit. Details of the target and priming specificity are included in the individual handbooks above.
Packaged, optimised and ready to use. Expect Better Data.
Cluster of differentiation 15 (CD15) is a carbohydrate adhesion molecule. Positive staining for CD15 and negative staining for leukocyte common antigen or other B- or T-cell lineage markers helps recognize Reed Sternberg cells (RSC) in Classical Hodgkin’s Lymphoma (CHL), and distinguishes it from Hodgkin-like neoplasms. CD15 does not stain mesotheliomas and is therefore most useful for distinguishing epithelial mesothelioma from adenocarcinoma.
CE-IVD marked in accordance with EU Directive 98/79/EC
Ideal for use in in vitro diagnostic workflows
Fixative-free preservative, no cross-linking of DNA
Preserve cf-DNA/ct-DNA for 30 days at ambient temperature and for up to 8 days at 37°C.
Preserve cf-RNA for 30 days at ambient temperature
Preserve Circulating Tumour Cells (CTCs) for 14 days at ambient temperature
No plasma volume loss after shipping/transportation
Prevent hemolysis allowing better separation of plasma
Prevent apoptosis of blood cells and fragmentation of genomic DNA
Produce high quality/quantity of plasma cf-DNA/ct-DNA/cf-RNA
Vacuumed to draw 8.4 mL of blood in 10 mL tubes
The procedure for plasma isolation using this kit is compliant with ISO 20186-3:2019
Norgen’s cf-DNA/cf-RNA Preservative Tubes Dx are closed, evacuated plastic tubes for the collection and the preservation of cf-DNA, circulating tumor DNA, cf-RNA and circulating tumor cells in human whole blood samples during storage and shipping. Using these tubes in combination with Norgen’s Plasma/Serum Cell-Free Circulating DNA Purification Kits or Norgen’s Plasma/Serum RNA Purification Kits enables the purification and concentration of an inhibitor-free cf-DNA/ct-DNA/cf-RNA in a very small elution volume.
These tubes are ideal for collecting and preserving cf-DNA and cf-RNA samples for in vitro diagnostic use for medical purposes.
Performance
Norgen’s cf-DNA/cf-RNA Preservative Tubes Dx abridge the collection/processing of whole blood for the subsequent purification of cf-DNA and/or cf-RNA. The cf-DNA/cf-RNA Preservative Tubes Dx preserve and inhibit the programmed cell-death (apoptosis) of the blood, hence preventing the release of intracellular DNA/RNA into plasma. Whole blood samples can be shipped and stored at room temperature for the subsequent processing of plasma. The cf/ct DNA and cf-RNA levels are stable for up to 30 days at room temperature. The cf-DNA is also stable for up to 8 days at 37°C.
Workflow
NOTE: This product is not available for sale in the United States. For the research use only version, see Cat. 63950
Up to 30 days at room temperature (15-25°C) Up to 8 days at 37°C
Length of cf-RNA Preservation
Up to 30 days at room temperature (15-25°C)
Length of CTCs Preservation
Up to 14 days at room temperature (15-25°C)
Storage Conditions and Product Stability
When stored at 15-30°C, unfilled Norgen cf-DNA/cf-RNA Preservative Tubes Dx are stable through the expiration date. See tube label for expiry date.
Short-term storage/shipping at -20°C to 37°C for up to 10 days is acceptable for unfilled Norgen cf-DNA/cf-RNA Preservative Tubes Dx.
Do not freeze unfilled Norgen cf-DNA/cf-RNA Preservative Tubes Dx for long term storage over 10 days. Freezing unfilled tubes may lead to loss of vacuum and precipitation may occur.
Cell-free DNA in blood samples collected in Norgen’s cf-DNA/cf-RNA Preservative Tubes Dx is stable for up to 30 days when stored at room temperature (15-25°C)
Cell-free DNA in blood samples collected in Norgen’s cf-DNA/cf-RNA Preservative Tubes Dx is stable for up to 8 days when stored at 37ºC.
Cell-free RNA in blood samples collected in Norgen’s cf-DNA/cf-RNA Preservative Tubes Dx is stable for up to 30 days when stored at room temperature (15-25°C)
Circulating tumor cells (CTCs) in blood samples collected in Norgen’s cf-DNA/cf-RNA Preservative Tubes Dx are stable for up to 14 days at room temperature (15-25ºC).
Suspend 111 g in 1 L of distilled or deionized water. Heat with frequent agitation and boil to completely dissolve the powder. Distribute into flasks. Autoclave at 121°C for 15 minutes. Cool to 50°C and pour into sterile Petri dishes.
Formula (per liter)
Pancreatic digest of casein—————5g Pancreatic digest of animal tissue——5g Beef extract————————————1g D-mannitol————————————-10g Sodium chloride—————————–75g Agar———————————————15g Phenolred——————————–0.025g Final pH———————————–7.4±0.2
Storage
Keep container tightly closed, store in a cool, dry place, away from bright light. Storage period of 3 years.
