Internal Lane Standard (60bp – 600bp, ROX) for ABI Genetic Analyzer
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Detail
Overview
Ready-to-use
Highly stable
Precise
22 discrete bands from 60 bp to 600 bp
High quality ready-to-use double-stranded DNA ladder derived by recombinant technology for precise size and mass determination in various fluorescence-detection instruments including different models of Applied Biosystems® PRISM® and Genetic Analyzers
22 discrete bands, ranging from 60 bp to 600 bp
The ladder is asymmetrically labelled with ROX that can be detected by excitation at 576 nm and fluorescent emission at 597 nm
Compatible with products for fragment analysis including Promega PowerPlex® 16 System
Ladder Properties: • 22 discrete bands, ranging from 60 bp to 600 bp
Storage: • Store at -20°C in small aliquots. For longer term storage, -70°C is recommended. • The product is light-sensitive. Minimize light exposure.
Other Products
C13110 HiPure DNA Mini Column II
Product Info
Document
Product Info
Introduction
Magen’s HiPure columns are prepared by high quality glass fiber filter membrane as raw materials through membrane cutting, membrane release, ring release, ring pressing, gland, weighing and other processes. HiPure nucleic acid adsorption columns have the characteristics of long-term stability and high binding capacity. Experiments show that the highest binding capacity and binding efficiency of HiPure nucleic acid adsorption columns are basically unchanged when stored at room temperature for 4 years.
The series of nucleic acid columns produced by Magen Biotech are based on carefully selected imported glass fiber membranes (GF/B, GF/D, GF/F). Columns production processes such as polypropylene injection molding materials, injection molding process, and downstream membrane packing and compression rings are strictly controlled. This is to ensure that the column has extremely high adsorption capacity and long-term stability. Compared with conventional products on the market, Magen’s columns are with varieties, and binding rate will not change when stored at room temperature for 4 years.
Details
Specifications
Features
Specifications
Recommended application
Plasmid Mini Preparation,gDNA/ RNA Extraction, DNA/RNA Clean Up
Preservation conditions
Room temperature
Stability
Up to 4 years
Filter membrane
High quality glass fiber filter GF/B, 4 layers
Membrane aperture
1.0μm
Maximum binding yield of plasmid
35 μg
Maximum yield of alcohol mediated Binding
200 μg
Single liquid carrying capacity of column
800 μl
Minimum elution volume
50 μl
Withstand centrifugal force
16,000 x g
Centrifuge
Small high speed centrifuge (2ml)
Adsorption Mechanism
Based on the negatively charged DNA skeleton, it has a high affinity for positively charged glass fibers. In high salt and ethanol solutions, DNA/RNA binds to glass fiber and interacts with hydrophilic matrix on silica through hydrogen bond. DNA/RNA is tightly bound. All pollutants can be removed by washing solution. At high salt concentration, nucleic acids selectively bind to silicagel membrane, while other pollutants, mainly proteins, are removed by membrane washing.
Ordering information
CAT.No.
Product Name
Package
C13110
HiPure DNA Mini Column II (4 x GF/B)with 2ml Collection Tubes
1000/Bag
Purchase Guide
Item No.
Product Name
Membrane type/number of layers
Collection tubes
Plasmid DNA binding capacity (Physical adsorption)
Note: GF/B pore size is for 1.0μM glass fiber membrane; GF/F pore size is for 0.7μm glass fiber membrane.
Document
Magen’s HiPure columns are prepared by high quality glass fiber filter membrane as raw materials through membrane cutting, membrane release, ring release, ring pressing, gland, weighing and other processes. HiPure nucleic acid adsorption columns have the characteristics of long-term stability and high binding capacity. Experiments show that the highest binding capacity and binding efficiency of HiPure nucleic acid adsorption columns are basically unchanged when stored at room temperature for 4 years.
The HiPure Minipreps system provides a fast, simple, and cost-effective plasmid DNA miniprep method for routine molecular biology laboratory applications. HiPure Miniprep Kits use silica membrane technology to eliminate the cumbersome steps associated with loose resins or slurries. Plasmid DNA purified with Mini Kits is immediately ready for use. Phenol extraction and ethanol precipitation are not required, and high quality plasmid DNA is eluted in a small volume of Tris buffer or water.
Details
Specifications
Features
Specifications
Main Functions
Isolation up to 30μg endotoxin-free plasmid DNA from 1-5ml bacterial culture using 96-well bind plate
Applications
Cell transfection, animal injection, etc.
Purification method
96 well plate
Purification technology
Silica technology
Process method
Manual (centrifugation or vacuum)
Sample type
Low copy plasmid vector
Sample amount
1-5ml LB（x96）
Yield
30μg
Elution volume
≥75μl
Time per run
≤60 minutes
Liquid carrying volume per column
800μl
Binding yield of column
70μg
Principle
The HiPure Plasmid procedure is based on alkaline lysis of bacterial cells followed by adsorption of DNA onto silica in the presence of high salt. The unique silica membrane used in the kit completely replaces glass or silica slurries for plasmid DNA minipreps. The procedure consists of 3 basic steps: Preparation and clearing of a bacterial lysate by alkaline method，then transfer the supernatant to column to bind DNA. After washing proteins and other impurities, nucleic acid was finally eluted with low-salt buffer (10mm Tris, pH9.0, 0.5mm EDTA).
Advantages
1. High throughput: processing 96 samples at once; 2. High yield: The purified plasmid yield can reach up to 30μg, which is greater than the yield of the magnetic bead method kit; 3. Detoxification: endotoxin content <0.1EU/μg; 4. Fast: The entire extraction can be completed in 60 minutes without the need for time-consuming alcohol precipitation;
Kit Contents
Contents
P115701
P115702
P115703
Purification Times
1 x 96 Preps
4 x 96 Preps
20 x 96 Preps
RNase A
10 mg
20 mg
100 mg
Buffer P1
30 ml
120 ml
550 ml
Buffer P2
30 ml
120 ml
550 ml
Buffer LEN3
15 ml
60 ml
300 ml
Buffer LN4
80 ml
270 ml
2 x 700 ml
Buffer LN5
40 ml
220 ml
1100 ml
Buffer PW1
40 ml
220 ml
1100 ml
Buffer PW2
25 ml
100 ml
2 x 200 ml
Elution Buffer
30 ml
60 ml
250 ml
Lysate Clear Plate
1
4
20
HiPure DNA Plate
1
4
20
1.6ml Collection Plate
2
8
40
0.5ml Collection Plate
1
4
20
Storage and Stability
The kit components can be stored dry at room temperature (15-25°C) and are stable for at least 18 months under these conditions. If any precipitates form in the buffers, warm at 37℃ to dissolve. After addition of RNase A，Buffer P1 is stable for 6 months when stored at 2-8°C.
The HiPure Minipreps system provides a fast, simple, and cost-effective plasmid DNA miniprep method for routine molecular biology laboratory applications. HiPure Miniprep Kits use silica membrane technology to eliminate the cumbersome steps associated with loose resins or slurries. Plasmid DNA purified with Mini Kits is immediately ready for use. Phenol extraction and ethanol precipitation are not required, and high quality plasmid DNA is eluted in a small volume of Tris buffer or water.
Animal Disease Kit is a diagnostic kit designed for animal detection. It is suitable for the detection of various diseases, including cat and dog test kits, in animals. The kit is composed of various components such as primers, probes, reagents, buffers and other materials necessary for the detection of diseases, which can be used for test strips and other detection methods. The kit can be stored at -20°C for long-term use. With this Animal Disease Kit, you can easily detect diseases in animals and protect them from harm.
The Animal Disease Kit includes multiple components, such as primers, probes, reagents, buffers, etc., to detect diseases. The kit offers various detection methods, including test strips, making it suitable for a broad range of applications in animal disease detection.
Animal Disease Kit is widely used in cattle veterinary medicine, animal disease detection, and animal feed. The kit has been proved to be reliable and efficient in the detection of animal diseases. It helps to diagnose diseases quickly, providing an accurate basis for subsequent treatments.
Support and Services:
We are committed to providing quality products and services to our customers. Our Animal Disease Kit is designed for veterinary professionals to diagnose and treat medical conditions in animals.
1.Technical Support
Our technical support staff are available to answer any questions you may have about the Animal Disease Kit. We provide assistance with product installation, troubleshooting, and any software or hardware issues you may encounter. We also offer technical advice on how to use the kit and its features.
2.Customer Service
Our customer service team is available to help you with any inquiries you may have about the Animal Disease Kit. We assist with product returns, replacement parts, and warranty information. We also provide product information and advice on how to get the most out of your Animal Disease Kit.
FAQ:
Q1: What is the “Animal Disease Kit” product?
A1: The “Animal Disease Kit” is a comprehensive toolkit for the diagnosis of animal diseases.
Q2: What does the Animal Disease Kit include?
A2: The Animal Disease Kit includes Primers, Probes, Reagents, Buffers, Etc.
Q3: How do I use the Animal Disease Kit?
A3: The Animal Disease Kit is designed for easy and convenient use. You can refer to the instructions manual for detailed instructions on how to use the product.
Q4: How accurate is the Animal Disease Kit?
A4: The Animal Disease Kit is designed for accurate and reliable results. It has been tested and certified by qualified professionals for accuracy and reliability.
Q5: Is the Animal Disease Kit safe to use?
A5: Yes, the Animal Disease Kit is safe to use. All components of the kit are made with non-toxic and non-irritating materials, and all reagents are safe to use.
Document
Animal Disease Kit is a diagnostic kit designed for animal detection. It is suitable for the detection of various diseases, including cat and dog test kits, in animals. The kit is composed of various components such as primers, probes, reagents, buffers and other materials necessary for the detection of diseases, which can be used for test strips and other detection methods. The kit can be stored at -20°C for long-term use. With this Animal Disease Kit, you can easily detect diseases in animals and protect them from harm.
