IST-104 PeelASeal FoilTM Heat Sealing Film

Description 

AccuBand™ 100 bp DNA Ladder II is composed of 10 individual DNA fragments, presenting 1k, 900, 800, 700 600, 500, 400, 300, 200 and 100 bp sharp bands respectively. This product contains 1 enhanced band (500 bp) for easy identification of bands. AccuBand™ 100 bp DNA Ladder II is ready-to-use, containing loading buffer with dual color tracking dyes (orange G and Xylene cyanol FF). AccuBand™ 100 bp DNA Ladder II provides a sufficient amount of DNA for clear observation of all DNA bands ranging from 100 bp to 1 kb, either in agarose gel or in polyacrylamide gel electrophoresis. 

Features

• Sharp bands
• Suitable for polyacrylamide gel electrophoresis
• Quick reference— enhanced bands 
• Ready-to-use— premixed with loading dye for direct loading
• Stable— room temperature storage over 6 months

Source 

Phenol extracted PCR products and dsDNA digested with specific restriction enzymes, equilibrated in 10 mM Tris-HCl (pH 8.0) and 10 mM EDTA.

Range 

100 ~ 1,000 bp 

Concentration 

50 µg/ 500 µl 

Recommended loading volume 

5 µl/ well

Storage

Room temperature for 6 months
4°C for 12 months 
-20°C for 36 months 

N-(Propargyl-PEG2)-DBCO-PEG3-Amine, TFA salt enables formation of triazole linkage with azide-bearing compound via copper catalyzed Click Chemistry. The amine group is reactive with carboxylic acids, activated NHS esters, carbonyls (ketone, aldehyde), etc. The propargyl group can be linked to azide-containing biomolecules via Click Chemistry. Reagent grade, for research purpose.

N-(Propargyl-PEG2)-DBCO-PEG3-Amine, TFA salt enables formation of triazole linkage with azide-bearing compound via copper catalyzed Click Chemistry. The amine group is reactive with carboxylic acids, activated NHS esters, carbonyls (ketone, aldehyde), etc. The propargyl group can be linked to azide-containing biomolecules via Click Chemistry. Reagent grade, for research purpose.

Overview

• Sample collection is non-invasive and painless
• Fast and easy processing using a magnetic bead system
• Isolate high quality genomic DNA
• This kit is also compatible with Norgen’s Saliva DNA Collection and Preservation Devices
• Also available in a 96-well format that can be integrated with a robotic automation system

Norgen’s Saliva DNA Isolation Kit (Magnetic Bead System) provides a fast and reproducible method for isolating genomic DNA from saliva samples collected and preserved using Norgen’s Saliva DNA Collection and Preservation Devices, as well as fresh saliva.  Saliva DNA purified using Norgen’s kit is of the highest quality, and is compatible with a number of downstream research applications including PCR, Southern Blot analysis, sequencing and microarray analysis.

Norgen’s Saliva DNA Isolation Kit (Magnetic Bead System) is also available in a 96-well format for high throughput applications. Purification with the 96-well plates can be integrated with a robotic automation system.

Details

Supporting Data

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Kit Specifications
Maximum Saliva Input	0.5 mL preserved saliva 0.25 mL fresh saliva
Average Yield from 0.25 mL of Saliva*	2-5 μg
Average Purity (OD260/280)	1.6 – 1.8
Time to Complete 10 Purifications	30 minutes (Cat. RU55400) 45 minutes (Cat. RU62900)

* Average DNA yield will vary depending on the donor

Storage Conditions and Product Stability
The kit contains ready-to-use Proteinase K which is dissolved in a specially prepared storage buffer. The Proteinase K should be stored at room temperature or 4°C. All other solutions should be kept tightly sealed and stored at room temperature (15 – 25°C). These reagents should remain stable for at least 2 years in their unopened containers.

Component	Cat. RU55400 (50 preps)	Cat. RU62900 (192 preps)
Lysis Buffer F	30 mL	2 x 30 mL
Proteinase K in Storage Buffer	1.2 mL	4 mL
Magnetic Bead Suspension	2 x 1.1 mL	8.5 mL
Elution Buffer B	8 mL	30 mL
Elution Tubes (1.7 mL)	50	–
96-Well Plate	–	2
96-Well Elution Plate	–	2
Adhesive Tape	–	2
Product Insert	1	1