Description 

The GetClone™ PCR Cloning Vector II is a positive selection system for high efficiency cloning of blunt end DNA or amplicons. This cloning vector contains a lethal gene which can be disrupted by ligation of a blunt end DNA insert into the cloning site. Only colonies with inserted vectors are able to propagate, eliminating the additional needs of IPTG and X-Gal for blue/white screening. This cloning vector includes ampicillin and kanamycin resistance genes that can meet the needs of most users. 

Features

• Cloning efficiency greater than 90% 
• IPTG and X-Gal are not required 
• Accepts a wide range of insert/vector ratios 0.5:1 to 12:1 
• Accepts insert size from 6 bp to 11 kb
• The phosphorylation of PCR fragments is not required
• Accepts blunt end amplicon or DNA fragment (not for sticky ends)
• Ampicillin and kanamycin selection markers

Storage

-20°C for 24 months

The GetClone™ PCR Cloning Vector II is a positive selection system for high efficiency cloning of blunt end DNA or amplicons. This cloning vector contains a lethal gene which can be disrupted by ligation of a blunt end DNA insert into the cloning site. Only colonies with inserted vectors are able to propagate, eliminating the additional needs of IPTG and X-Gal for blue/white screening. This cloning vector includes ampicillin and kanamycin resistance genes that can meet the needs of most users.

Introduction

HiPure Insect DNA Kits provides a simple and rapid solution for total DNA extraction of insect tissue samples. This kit is based on silica gel column purification technology without toxic phenol chloroform extraction and time-consuming alcohol precipitation. The whole extraction process only takes 30 minutes. HiPure Insect DNA Kit can process tissue samples less than 10mg at a time. Hipure Insect DNA 96 kit can process 96 insect tissue samples at a high throughput. The obtained DNA can be directly used in PCR, Southern blot, viral DNA detection and other experiments.

Details

Specifications

Features	Specifications
Main Functions	Isolation total DNA from insect tissue
Applications	PCR, southern bolt and virus detection, etc
Purification method	96 well DNA plate
Purification technology	Silica technology
Process method	Manual (centrifugation or vacuum)
Sample type	Insect tissue samples
Sample amount
Elution volume
Time per run
Liquid carrying volume per column
Binding yield of column

Principles

This product is based on silica column purification. The sample is lysed and digested with lysate and protease, DNA is released into the lysate. Transfer to an adsorption column. Nucleic acid is adsorbed on the membrane, while protein is not adsorbed and is removed with filtration. After washing proteins and other impurities, Nucleic acid was finally eluted with low-salt buffer (10mm Tris, pH9.0, 0.5mm EDTA).

Advantages

• High purity DNA – can be used in sensitive downstream applications such as multiplex and quantitative pcr
• Good repeatability – suitable for extracting high-yield DNA from insect tissue samples
• Safe – without phenol chloroform extraction and alcohol precipitation

Kit Contents

Contents	D313901	D313902
Purification Times	1 x 96	4 x 96
HiPure DNA Plate	1	4
2.2 ml Collection Plate	1	4
1.6 ml Collection Plate	1	4
0.5ml Collection Plate	1	4
Seal Film	8	32
Buffer ITL	30 ml	120 ml
Buffer IL	30 ml	125 ml
Buffer GW1	44 ml	2 x 110 ml
Buffer GW2	50 ml	3 x 50 ml
Proteinase K	50 mg	200 mg
Protease Dissolve Buffer	6 ml	15 ml
Buffer AE	20 ml	60 ml

Storage and Stability

Proteinase K, RNase A should be stored at 2-8°C upon arrival. However, short-term storage (up to 12 weeks) at room temperature (15-25°C) does not affect their performance. The remaining kit components can be stored at room temperature (15-25°C) and are stable for at least 18 months under these conditions. The entire kit can be stored at 2–8°C, but in thiscase buffers should be redissolved before use. Make sure that all buffers areat room temperature when used.

HiPure Insect DNA Kits provides a simple and rapid solution for total DNA extraction of insect tissue samples. This kit is based on silica gel column purification technology without toxic phenol chloroform extraction and time-consuming alcohol precipitation. The whole extraction process only takes 30 minutes. HiPure Insect DNA Kit can process tissue samples less than 10mg at a time. Hipure Insect DNA 96 kit can process 96 insect tissue samples at a high throughput. The obtained DNA can be directly used in PCR, Southern blot, viral DNA detection and other experiments.

Overview

• Reliable and cost-effective
• Non-invasive, user-friendly, convenient sample collection & preservation in a single tube system
• Preserved exosomes are stable for 2 years at ambient temperature
• Shipping accessories can be purchased separately
• Compatible with Norgen’s Saliva Exosome Purification Kit (Cat. 65300)
• RNA can be isolated from the purified exosomes using Norgen’s Exosome RNA Purification kit (Cat. 58000)

Norgen’s Saliva Exosome Collection and Preservation Kit is an all-in-one solution designed for 1) simple and non-invasive saliva collection; 2) preservation of exosomes in saliva samples at ambient temperature; and 3) isolation of high quality intact exosomes and exosomal RNA within a laboratory setting. The Saliva Exosome Collection, Preservation and Isolation Kit contains 50 Individual Saliva Exosome Collection and Preservation Devices. Each of the 50 individual Saliva Exosome Collection and Preservation Devices consists of 3 components:

• Saliva Collection Funnel and Collection Tube that contains Norgen’s Preservative in a dried format
• Collection Tube Cap
• ID Label

Saliva samples are collected by spitting inside the Collection Funnel which has been assembled with the Collection Tube. After collecting the required volume of saliva the Collection Funnel is removed, the Collection Tube is securely capped with Collection Tube Cap. The collected saliva is mixed by gentle inversion for several times till the saliva colour changes into yellow. The Saliva Collection Tube is subsequently sent to the laboratory for the purification of intact exosomes and for the subsequent isolation and analysis of saliva exosomal RNA. The saliva exosomes in preserved samples are stable for 2 years at room temperature.

Details

Kit Specifications
Volume of Saliva Collected	2 mL
Preservation Temperature	Room Temperature
Preservation time	2 years at room temperature

Shelf Life and Handling

• The Collection Device should be kept tightly sealed and stored at room temperature for up to 2 years without any reduction in kit performance (The collection due date is written on the device label).
• The Collection Tube, the Collection Funnel and the Device Container of each Individual Saliva DNA Collection and Preservation Device are recyclable.

Kit Components	Cat. 65400 (50 Devices)
Individual Saliva Exosome Collection and Preservation Devices	50
Donor Procedure Flowchart	1
Product Insert	1
Individual Saliva Exosome Collection and Preservation Device Contents
Saliva Collection Funnel and Collection Tube	1
Collection Tube Cap	1
Donor Instructions	1