IST-121 QuickSeal qPCR CrystalTM Self Adhesive Sealing Film
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Optically clear adhesive film with pressure activated adhesive. Seal is peelable; suitable for qPCR and other imaging techniques including crystallization.
Detail
Overview
Optically clear adhesive film with pressure activated adhesive. Seal is peelable; suitable for qPCR and other imaging techniques including crystallization.
Optically clear seal specifically developed for optical applications, particularly qPCR
It is non sticky when removed from the packaging; this aids handling when wearing gloves
The adhesive is contained within small capsules, allowing light to pass through to ensure the optical clarity of the seal
When the seal is in position, pressure can be applied to burst the capsules, releasing a strong adhesive only where the seal touches the raised well rims of the plate – the rest of the seal area above the wells remains optically clear
For all adhesive seals, the best sealing results are achieved using our Hand Roller or KAPS 500 Auto Sealer
Other Products
HighRanger 1 kb DNA Ladder
Product Info
Document
Product Info
Overview
Ready-to-use
Quantitative
Precise
Highly Stable
Thirteen discrete fragments ranging from 300 bp to 10000 bp
Higher intensity reference band at 5000 bp
The HighRanger 1 kb DNA Ladder is prepared to ensure quality and batch-to-batch consistency. This Ladder contains thirteen discrete fragments ranging from 300 bp to 10,000 bp with a higher intensity reference band at 5000 bp. This Ladder is ideal for quick sizing of PCR products and restriction digests.
Contents 1mL of premixed DNA ladder (0.5µg/10µL) in loading buffer (10mM EDTA, 10% glycerol, 0.015% bromophenol blue, and 0.17% SDS).
HighRanger 1kb DNA Ladder (Cat# 11900) – 100 loads
Ladder Properties: • Thirteen discrete bands, ranging from 300 bp to 10000 bp • Higher intensity band at 5000 bp for easy reference
Fragment
Size (bp)
Mass (ng)
1
10000
58
2
8000
53
3
6000
46
4
5000
67
5
4000
56
6
3000
47
7
2500
41
8
2000
34
9
1500
29
10
1000
18
11
700
13
12
500
23
13
300
14
Recommended Use:
Mix thoroughly. For best results, load 10µL of DNA ladder per well. For precise mass determination with a densitometer, stain gel after electrophoresis using 0.5µg/mL ethidium bromide for 30-40 minutes. The table above shows the size and mass for each band based on 10µL ladder per well.
Storage:
This kit is stable for 2 years after the date of shipment.
This ladder was standardized using 10µL of DNA per lane on a 0.8 cm thick, 13 x 15 cm, 1.0% agarose gel run in TAE buffer.
Propargyl-PEG3-t-butyl ester consists of an alkyne group and a t-butyl ester group. The alkyne groupreacts with azide-bearing compounds or biomolecules in copper catalyzed Click Chemistry reactions. The protected carboxyl group can be deprotected under acidic conditions. Reagent grade, for research purpose. Please contact us for GMP-grade inquiries.
Document
Propargyl-PEG3-t-butyl ester consists of an alkyne group and a t-butyl ester group. The alkyne groupreacts with azide-bearing compounds or biomolecules in copper catalyzed Click Chemistry reactions. The protected carboxyl group can be deprotected under acidic conditions. Reagent grade, for research purpose. Please contact us for GMP-grade inquiries.
The IdPath™ COVID-19 Real-Time RT-PCR Kit is a real-time RT-PCR test intended for the detection of SARS-CoV-2 virus RNA which might be extracted from the respiratory tract specimens. The Kit provides reagents for multiplex real-time RT-PCR to detect SARS-CoV-2 by one-step reaction, specifically targeting the E (Envelope), RdRP (RNA-dependent RNA polymerase) and N (Nucleocapsid protein) gene for SARS-CoV-2 virus.
The Kit contains the RT enzyme mix and qPCR master mix for reverse transcription and real-time PCR of virus RNA. The COVID-19 Control (positive control) and ddWater (negative control) are used as indicators to avoid false negative/positive results across all experimental procedures. The Primers/probes Mix contains multiplex primers and TaqMan probes specific to the N, E/RdRP genes of SARS-CoV-2 and RNase P gene of human, detected by FAM, VIC and ROX channels, respectively.
For Research Use Only
Features
High Sensitivity:5×102 copies/ml (10 copies/rxn)
High Inclusivity : >99% of currently available complete virus genomes for SARS-CoV-2 including Omicron variant
High Accuracy : Clinical validation with 100% accuracy
High Stability : 37/25°C for 2 weeks ; 4°C for 24 weeks ; 10 times of freeze-thaw cycles
High Compatibility : Suitable for most laboratory qPCR machines
Operation Control : Including internal control for quality control of total process
Convenience : Multiplex (E/RdRP and N) detection by one-step reaction
Storage
Aliquot to avoid multiple freeze-thaw cycles
Protect fluorogenic probes from light
-20°C for 12 months
Document
The IdPath™ COVID-19 Real-Time RT-PCR Kit is a real-time RT-PCR test intended for the detection of SARS-CoV-2 virus RNA which might be extracted from the respiratory tract specimens. The Kit provides reagents for multiplex real-time RT-PCR to detect SARS-CoV-2 by one-step reaction, specifically targeting the E (Envelope), RdRP (RNA-dependent RNA polymerase) and N (Nucleocapsid protein) gene for SARS-CoV-2 virus.
The Kit contains the RT enzyme mix and qPCR master mix for reverse transcription and real-time PCR of virus RNA. The COVID-19 Control (positive control) and ddWater (negative control) are used as indicators to avoid false negative/positive results across all experimental procedures. The Primers/probes Mix contains multiplex primers and TaqMan probes specific to the N, E/RdRP genes of SARS-CoV-2 and RNase P gene of human, detected by FAM, VIC and ROX channels, respectively.