A transparent, optically clear, DMSO resistant, non-tacky film which adheres only when pressure is applied. It is non-pierceable and peelable. Clear plastic, reflective, glossy on the top. Very thin and light and doesn’t crease easily.
Detail
Overview
A transparent, optically clear, DMSO resistant, non-tacky film which adheres only when pressure is applied. It is non-pierceable and peelable. Clear plastic, reflective, glossy on the top. Very thin and light and doesn’t crease easily.
Crystal clear seal specifically developed for optical applications, particularly qPCR
Non-sticky when removed from the packaging aiding with handling when using gloves
When the seal is in position, pressure can be applied to burst the capsules, releasing a strong adhesive only where the seal touches the raised well rims of the plate – the rest of the seal area above the wells remains optically clear
Urine Exfoliated Cell and Bacteria RNA Purification Kit
Product Info
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Product Info
Overview
Purify all sizes of RNA (including microRNA) without the need for phenol
Isolate and detect total RNA from 1 mL and up to 50 mL urine
Provides high-quality RNA for sensitive applications – isolate RNA from as little as 100 cells
Rapid processing time from sampling to downstream testing
Purification is based on spin column chromatography that uses Norgen’s proprietary resin separation matrix
This kit provides a rapid spin column method for the isolation and purification of total RNA (including microRNA) from exfoliated cells that have been shed into the urine from the urinary tract. The kit purifies all sizes of RNA, from large mRNA and ribosomal RNA down to microRNA (miRNA) and small interfering RNA (siRNA). The RNA is preferentially purified from other cellular components such as proteins, as well as from the contaminating species found in urine such as glucose and salts, without the use of phenol or chloroform. The purified RNA is of the highest integrity and can be used in a number of downstream applications including real-time RT-PCR, qRT-PCR, Northern blotting, NGS, and expression array assays. Multiple samples can be processed in 20 minutes.
Background
RNA biomarkers from exfoliated cells can be used as non-invasive tools for a number of diagnostic and research applications including the diagnosis and monitoring of bladder, kidney, or other urinary-tract cancers.
~1μg RNA per 1 x 105 cells (For Exfoliated Cells – Varies due to cell density sample)~0.5μg RNA per 1 x 107 cells(For Bacteria – Varies due to cell density sample)
Storage Conditions and Product Stability All solutions should be kept tightly sealed and stored at room temperature. This kit is stable for 2 years after the date of shipment.
