Virgin Polypropylene 96 Square Well plate with high uniformity from well to well.
Permagen’s MSPU650R is identical to the product shown above, with the exception of the added integrated cushion base which helps aid in robot and consumable labware inconsistencies for precision pipetting
Features include solid aluminum alloy construction and hard coat anodized finish for years of trouble-free use, and compatible with any magnetic beads
MSPU650R
Minimum – 5 µL (PCR Plate)
Maximum – 2 mL (Deep Well Plate)
Permagen’s MSPU650R is identical to the product shown above, with the exception of the added integrated cushion base which helps aid in robot and consumable labware inconsistencies for precision pipetting
N-(Boc-PEG1)-N-bis(PEG2-propargyl) is a click chemistry branched linker. The propargyl groups can react with azide-bearing molecule via copper catalyzed Click Chemistry . The Boc group can be deprotected under acidic conditions to release amine group. Reagent grade, for research purpose. Please contact us for GMP-grade inquiries.
N-(Boc-PEG1)-N-bis(PEG2-propargyl) is a click chemistry branched linker. The propargyl groups can react with azide-bearing molecule via copper catalyzed Click Chemistry . The Boc group can be deprotected under acidic conditions to release amine group. Reagent grade, for research purpose. Please contact us for GMP-grade inquiries.
K-PullG6
SKU: 700004329
100/200 assays per kit
| Content: | 100 / 200 assays per kit |
| Shipping Temperature: | Ambient |
| Storage Temperature: | Short term stability: 2-8oC, Long term stability: See individual component labels |
| Stability: | > 2 years under recommended storage conditions |
| Analyte: | Pullulanase/Limit-Dextrinase |
| Assay Format: | Spectrophotometer |
| Detection Method: | Absorbance |
| Wavelength (nm): | 400 |
| Signal Response: | Increase |
| Limit of Detection: | 0.18 U/mL for pullulanase preparations (50-fold dilution) 0.01 U/g for limit dextrinase in milled malt |
| Reproducibility (%): | ~ 3% |
| Total Assay Time: | ~ 10 min (Pullanase), ~ 30 min (Limit-Dextrinase) |
| Application examples: | Assay of microbial pullulanase preparations. Measurement of limit-dextrinase in malt extracts. |
| Method recognition: | Novel method |
PullG6 assay for the measurement of pullulanase employs a water soluble defined substrate, namely 4,6-O-benzylidene-4-nitrophenyl-63-α-D-maltotriosyl-maltotriose (BPNPG3G3), coupled with the ancillary enzymes α-glucosidase and β-glucosidase. Upon hydrolysis of the substrate at the 1,6-α-linkage by pullulanase or limit-dextrinase, the released 4-nitrophenyl-β-maltotrioside is immediately hydrolysed to glucose and 4-nitrophenol by the concerted action of the α-glucosidase and β-glucosidase enzymes in the reagent mixture. The reaction is terminated and phenolate ions are developed by addition of dilute alkali. The absorbance is read at 400 nm and the value obtained correlates directly with pullulanase activity.
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