Introduction

This Kit is designed forpurification of high quality circulating DNA (cfDNA) from 1-6ml cell-free bodyfluids (such as plasma, serum). The purified DNA is suitable for direct use indownstream applications such as PCR, real-time PCR, Biochip analysis and NGS.

Details

Specifications

Features	Specifications
Main Functions	Isolation circulating DNA from 1-6ml plasma, serum, body fluids
Applications	qPCR, NGS, etc.
Purification technology	Magnetic beads technology
Process method	Manual or automatic
Sample type	Serum, plasma
Sample amount	1-6ml
Elution volume	≥50μl
Time per run	≤60 minutes

Principle

This product is based on the purification method of high binding magnetic particles. The sample is lysed and digested under the action of lysate and protease. DNA is released into the lysate. After adding magnetic particles and binding solution, DNA will be adsorbed on the surface of magnetic particles, and impurities such as proteins will be removed without adsorption. The adsorbed particles were washed with washing solution to remove proteins and impurities, washed with ethanol to remove salts, and finally DNA was eluted by elution buffer.

Advantages

• Economy – less than 50% of the price of Qiagen and other imported products
• Automatic – without labour
• High purity – OD 260 / 280 : 1.7-1.9, OD 260 / 230 :1.5-2.0

Kit Contents

Contents	IVD5435
Purification Times	50
MagPure Particles F	14 ml
Carrier RNA	310 μg
Proteinase K	240 mg
Protease Dissolve Buffer	15 ml
Buffer SDS	15 ml
Buffer MLK	500 ml
Buffer MAW1	250 ml
Buffer MW2*	50 ml
Elution Buffer	60 ml

Storage and Stability

Proteinase K, Carrier RNA and MagPure Particles F should be stored at 2–8°C upon arrival. However, short-term storage (up to 12 weeks) at room temperature (15–25°C) does not affect their performance.Theremaining kit components can be stored dry at room temperature (15–25°C) and are stable for at least 18 months under these conditions.The entire kit can be stored at 2–8°C, but in this case buffers should beredissolved before use. Make sure that all buffers are at room temperature when used.

Experiment Data

This Kit is designed forpurification of high quality circulating DNA (cfDNA) from 1-6ml cell-free bodyfluids (such as plasma, serum). The purified DNA is suitable for direct use indownstream applications such as PCR, real-time PCR, Biochip analysis and NGS.

Product advantages

1. The instantaneous centrifugal function rises to the maximum speed for about 6 seconds, allowing for rapid detachment of hanging droplets from the wall;

2. Faster acceleration and deceleration rate, required to complete the experiment in a shorter time;

3. Door cover protection, overspeed and imbalance detection system, capable of real-time monitoring of  the centrifugal process, ensuring the safe operation    of the  instrument; When the operation ends, an error occurs, or an imbalance occurs, the sound signal prompts, and the operation stops at the same time. The LCD displays the result code

Host Parameters

Product model	Mini-3
Input power supply	DC24V/2.75A
Input power	55W
Motor/Drive Method	DC24V brushless DC variable frequency motor
Display method	High brightness LCD screen
Effective centrifugal time	1-99min.1-59sec
speed	300~3000±15rpm
Speed step increase	10rpm
Maximum capacity	2 *96 well PCR plates/ELISA plates
MAX. RCF	608 xg
Misoperation/alarm failure	Sound prompt+display code
weight	3.9Kg
Noise	60dB (A)
Fastest acceleration time	<6s
Fastest deceleration time	<5s
Permissible ambient temperature/relative temperature	+5-40°C/80%

The micro porous plate centrifuge adopts international advanced design concepts and manufacturing technology, with a smooth and beautiful appearance, compact and stable structure, adhering to the advantages of high universality and easy operation. It also has the functional characteristics of “smooth start” and “smooth braking”; This micro porous plate centrifuge is very suitable for 96 or 384 well and small capacity micro porous plates, as well as various standard PCR micro porous plates with or without skirts .

Permagen’s MSPU650R is identical to the product shown above, with the exception of the added integrated cushion base which helps aid in robot and consumable labware inconsistencies for precision pipetting  

Features include solid aluminum alloy construction and hard coat anodized finish for years of trouble-free use, and compatible with any magnetic beads

SKU #

MSPU650R

Features

• SBS/ SLAS footprint
• Solid Aluminum alloy design
• Precision manufactured for more consistent results
• Fast magnetic bead separations
• Integrated corner brackets to help keep microplate in place
• Cushion base allowing tips to come in contact with well bottoms
• Made in USA 
• For Research use only

Compatibility

• Round bottom plates 
• Pyramid bottom plates 
• Flat Bottom plates
• Deep well plates
• PCR Plates (Full-skirt)
• Any magnetic beads
• Any common liquid handlers i.e. Tecan®, Opentrons®, Hamilton®, Agilent®, Beckman®, etc.

Volumes

Minimum – 5 µL (PCR Plate)

Maximum – 2 mL (Deep Well Plate)

Permagen’s MSPU650R is identical to the product shown above, with the exception of the added integrated cushion base which helps aid in robot and consumable labware inconsistencies for precision pipetting