endo-BCN-PEG2-Acrylate is a PEG linker containing a BCN group and an acrylate group. The BCN group enables copper free click chemitry with azide-tagged molecules. The acrylate group enables Michael addition reactions. The hydrophilic PEG spacer increases solubility in aqueous media. Reagent grade, for research use only.

endo-BCN-PEG2-Acrylate is a PEG linker containing a BCN group and an acrylate group. The BCN group enables copper free click chemitry with azide-tagged molecules. The acrylate group enables Michael addition reactions. The hydrophilic PEG spacer increases solubility in aqueous media. Reagent grade, for research use only.

Introduction

MagPure Stool DNA Kit is specially designed for high throughput DNA extraction from stool samples. It can get high purity microbial DNA from stool samples (≤200mg). This kit is based on magnetic beads purification and unique inhibiting factors adsorption technology, no phenol-chloroform extraction or alcohol precipitation. It can adsorb humic acid and other inhibiting factors in the solution efficiently. DNA can be directly used for downstream applications such as PCR, Viral DNA testing, bacterial DNA testing, etc.

Details

Specifications

Features	Specifications
Main Functions	Isolation total DNA from 100-150mg stool samples
Applications	PCR, southern blot and enzyme digestion, etc.
Purification technology	Magnetic beads technology
Process method	Manual or automatic
Sample type	Stool
Sample amount	100-150 mg
Elution volume
Time per run

Principle

This product is based on the purification method of high binding magnetic particles. The sample is lysed and digested under the action of lysate and Lysozyme. DNA is released into the lysate. After adding magnetic particles and binding solution, DNA will be adsorbed on the surface of magnetic particles, and impurities such as proteins will be removed without adsorption. The adsorbed particles were washed with washing solution to remove proteins and impurities, washed with ethanol to remove salts, and finally DNA was eluted by Elution Buffer.

Advantages

• Fast – several samples can be extracted in 60 minutes (after digestion)
• High purity – unique adsorbent can completely remove inhibitory factors
• High recovery – DNA can be recovered at the level of PG

Kit Contents

Contents	D636401	D636402	D636404
Purification Times	48 Preps	96 Preps	400 Preps
MagPure Particles N	1.7 ml	3.4 ml	14 ml
2ml Bead Tubes	48	96	400
RNase A	10 mg	20 mg	75 mg
Proteinase K	24 mg	48 mg	180 mg
Protease Dissolve Buffer	3 ml	5 ml	20 ml
Buffer ATL	60 ml	110 ml	300 ml
PVP-10	1.2 g	2.2 g	6 g
Buffer PCI	50 ml	100 ml	300 ml
Buffer MLE	30 ml	60 ml	180 ml
Buffer GW1*	22 ml	44 ml	132 ml
Elution Buffer	20 ml	20 ml	60 ml

Storage and Stability

MagPure Particles, RNase A and Proteinase K should be stored at 2-8°C upon arrival. However, short-term storage (up to 24 weeks) at room temperature (15-25°C) does not affect their performance. The remaining kit components can be stored dry at room temperature (15-25°C) and are stable for at least 18 months under these conditions.

MagPure Stool DNA Kit is specially designed for high throughput DNA extraction from stool samples. It can get high purity microbial DNA from stool samples (≤200mg). This kit is based on magnetic beads purification and unique inhibiting factors adsorption technology, no phenol-chloroform extraction or alcohol precipitation. It can adsorb humic acid and other inhibiting factors in the solution efficiently. DNA can be directly used for downstream applications such as PCR, Viral DNA testing, bacterial DNA testing, etc.

Overview

• CE-IVDR marked in accordance with the European Commission Regulation (EU) No. 2017/746.
• Available for sale in Canada (Dx17200, Dx24300)
• Ideal for use in in vitro diagnostic workflows
• Extract high quality & quantity total RNA including miRNA
• No phenol step required
• Bind & elute all RNA irrespective of size or GC content, without bias
• Very sensitive & linear down to a few cells without the need for carrier RNA
• Convenient & fast spin column format
• Available in 96-Well and 96-Well (Deep Well) formats for high throughput automation
• Isolate from a wide variety of specimens
• Purified RNA is suitable for a variety of downstream applications
• Purification is based on spin column chromatography that uses Norgen’s proprietary resin separation matrix

Norgen’s Total RNA Purification Kits Dx provide rapid methods for the isolation and purification of total RNA from tissue samples, blood, plasma, serum, saliva, bacteria, yeast, fungi and viruses for subsequent in vitro diagnostic use. Extract high quality and purity RNA with excellent RIN values and A260/A280 suitable for downstream applications including qRT-PCR, RT-PCR, microarrays, NGS and more.

Total RNA Purification Kit Dx (Spin Column)

This kit is designed to be used with any downstream application employing enzymatic amplification or other enzymatic modifications of RNA followed by signal detection or amplification. Any diagnostic results generated using the RNA isolated with Norgen’s Total RNA Purification Kit Dx in conjunction with an in vitro diagnostic assay should be interpreted with regard to other clinical or laboratory findings.

Total RNA Purification 96-Well Kits Dx (High Throughput and High Throughput Deep Well)

Thess 96-well kits provide a rapid method for the high-throughput isolation and purification of total RNA in 30 minutes using vacuum manifold, plate centrifuge, or liquid handlers with vacuum capabilities. Total RNA can be isolated from a broad range of sample sources including cultured cells, tissues, blood, serum, plasma, bacteria, yeast, fungi, and viruses.

Intended Use

To minimize irregularities in diagnostic results, suitable controls for downstream applications should be used. Norgen’s Total RNA Purification Kits Dx are intended for use by professional users such as technicians, physicians and biologists experienced and trained in molecular biological techniques. Norgen’s Total RNA Purification Kits Dx do not provide a diagnostic result. It is the sole responsibility of the user to use and validate the kit in conjunction with a downstream in vitro diagnostic assay.

NOTE: This product is not available for sale in the United States.

Details

Supporting Data

Figure 1 / 13

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Kit Specifications
Maximum Binding Capacity Per Well	50 μg
Maximum Loading Volume Per Well	500 μL
Size of RNA Purified	All sizes, including small RNA < 200 nt
Time to Complete 96 Purifications	30 minutes
Average Yield HeLa Cells (1 x 106 Cells) E. coli (1 x 109 cells) Brain (10 mg) Liver (10 mg) Blood (50 μL, Hamster)	15 μg 50 μg 9 μg 26 μg 1 μg
Maximum Amount of Starting Material:  Whole Blood Plasma/Serum Bacteria Yeast Fungi Plant Tissue	100 µL150 µL1 x 109 cells1 x 108 cells 40 mg 40 mg

Storage Conditions and Product Stability
All solutions should be kept tightly sealed and stored at room temperature.  These reagents should remain stable for at least 1 year in their unopened containers.

Component	Cat. Dx17200 (50 preps)	Cat. Dx24300 (192 preps)	Cat. Dx24350 (192 preps)	Cat. Dx24380 (576 preps)
Lysis Solution	40 mL	–	–	–
Buffer RL	–	2 x 40 mL	2 x 40 mL	350 mL
Wash Solution	22 mL	–	–	–
Wash Solution A	22 mL	2 x 38 mL	2 x 38 mL	1 x 148 mL 1 x 74 mL
Elution Solution	6 mL	–	–	–
Elution Solution A	–	2 x 20 mL	2 x 20 mL	60 mL
Mini Spin Columns	50	–	–	–
96-Well Isolation Plate	–	2	2	–
96-Well Isolation Plate (Deep Well)	–	–	–	6
Collection Tubes	50	–	–	–
96-Well Collection Plate	–	2	2	–
96-Well Collection Plate (Deep Well)	–	–	–	6
Elution Tubes (1.7 mL)	50	–	–	–
96-Well Elution Plate	–	2	2	–
96-Well Elution Plate (Deep Well)	–	–	–	6
Adhesive Tape	–	4	4	12
Product Insert	1	1	1	1