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Lactose/Sucrose/D-Glucose Assay Kit

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The Lactose/Sucrose/D-Glucose assay kit is suitable for the measurement and analysis of sucrose, lactose and D-glucose in flour mixtures and other materials.

Detail

K-LACSU

100 assays of each per kit

Content:100 assays of each per kit
Shipping Temperature:Ambient
Storage Temperature:Short term stability: 2-8oC,
Long term stability: See individual component labels
Stability:> 2 years under recommended storage conditions
Analyte:D-Glucose, Lactose, Sucrose
Assay Format:Spectrophotometer
Detection Method:Absorbance
Wavelength (nm):510
Signal Response:Increase
Limit of Detection:100 mg/L
Reaction Time (min):~ 60 min
Application examples:Flours, beverages, dairy products, milk, foodstuffs containing milk, cosmetics, pharmaceuticals and other materials (e.g. biological cultures, samples, etc.).
Method recognition:Used and accepted in food analysis

This product has been discontinued (read more).

The Lactose/Sucrose/D-Glucose assay kit is suitable for the measurement and analysis of sucrose, lactose and D-glucose in flour mixtures and other materials.

Explore our full range of mono/disaccharide assay kits.

Scheme-K-LACSU LACSU Megazyme

Advantages

  • Very competitive price (cost per test) 
  • All reagents stable for > 12 months after preparation 
  • Simple format 
  • Very specific 
  • Rapid reaction 
  • Mega-Calc™ software tool is available from our website for hassle-free raw data processing 
  • Standard included

Other Products

Detergent-Free Total Protein Isolation Kit

Overview

  • Simple protocol that is free of detergents
  • Fast processing time
  • Process a variety of inputs
  • Compatible with mass spectrometry and NMR spectroscopy
  • Purification is based on spin column chromatography that uses Norgen’s proprietary resin separation matrix

This kit provides a fast and simple procedure for the isolation of total proteins from tissue, bacteria, yeast or mammalian cells without the use of SDS, Triton® X-100 and other detergents. Detergents are extensively used to prepare protein samples; however, these detergents have undesirable effects on downstream analysis. These effects include extraneous peaks in mass spectrometry, artifacts with chromatography and electrophoresis, interference with microinjection into cells and interference with protein immunization. 

The Detergent-Free Total Protein Isolation Kit maintains high protein recovery and yields proteins that are 100% detergent-free.  Purification is based on using Norgen’s proprietary resin together with Lysis Solution, followed by protein filtration using a filter column (provided). The purified proteins can be used in a number of downstream applications including mass spectrometry, SDS-PAGE, isoelectric focusing, NMR spectroscopy and more.

Each Lysis Tube is able to process up to 50 mg of tissue, 1010 bacterial cells, 109 yeast cells or 107 mammalian cells. Preparation time for 12 samples is less than 10 minutes. 

Details

Supporting Data

Figure 1 / 1

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Figure 1.  Isolation of Total Proteins
Figure 1.  Isolation of Total Proteins

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Kit Specifications
Maximum Amount Of Starting Material:
Tissues
Animal Cells
Bacteria
Yeast
50 mg
1 x 107 cells
1 x 1010 cells
1 x 109 cells
Time to Process 12 SamplesLess than 10 minutes


Storage Conditions and Product Stability
The Lysis Solution should be kept tightly sealed and stored at room temperature. Once opened, the solution should be stored at 4°C. This kit is stable for 2 years after the date of shipment.

ComponentCat. 30300 (50 preps)
Lysis Solution4 mL
Lysis Tubes25
Filter Columns25
Elution Tubes (1.7 mL)25
Product Insert1

16S V1-V2 Library Preparation Kit for Illumina

Overview

  • Protocol optimized for DNA isolated from a diversity of samples including stool, soil, water, saliva, plant, urine, skin, and more
  • Simple and quick workflow: library could be prepared in less than 5 hours
  • Component of Norgen’s metagenomics workflow
  • A single NGS run can be prepared with up to 384 unique dual-index libraries

Sample type purification kit guide 

The 16S V1-V2 Library Preparation Kit for Illumina consists of the reagents and components required for library preparation of the 16S V1-V2 amplicon libraries to be used for next-generation sequencing on Illumina platforms. All molecular reagents including primers, enzyme mixes, indexes, and buffers are provided. Instructions for PCR clean up with the AMPure XP Magnetic Beads (supplied by customer) are also included for rapid purification of nucleic acid products generated at two steps of the workflow. The library prep workflow could be used for purified DNA inputs from different sources including stool, soil, water, saliva, plant, urine, skin swab, vaginal swab, cheek swab, nasal swab, plasma/serum, tongue swab, gum swab, and others.

The 16S V1-V2 Library Preparation Kit for Illumina has a streamlined procedure that reduces the handling time such that the library prep procedure can be completed in approximately 4 hours (see diagram below). Input DNA is first subjected to targeted PCR to amplify the V1-V2 region of the DNA encoding 16S rRNA. The post-PCR reaction is then cleaned up using AMPure XP beads. Dual index primers are then added using a limited-cycle PCR. The indexed amplicons flanked by 5′ and 3′ barcoded adaptors are then cleaned using AMPure XP beads. The libraries are then ready for quantification, pooling and sequencing.16S Library Prep

Details

Supporting Data

Figure 1 / 3

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Figure 1: 16S V1-V2 PCR1 Amplification
Figure 1: 16S V1-V2 PCR1 Amplification
Figure 2: 16S V1-V2 Indexed Libraries
Figure 3: BioAnalyzer Trace of the 16S V1-V2 Indexed Library

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Minimum amount of starting material:2.5 µL of DNA (5 ng/µL)
Time to complete library preparation:4 hours

Storage Conditions and Product Stability
Norgen’s 16S V1-V2 Library Prep Kit for Illumina is shipped as one kit box (for the 24 prep kit) or two sub-component kits (for the 96 prep kit). All kits should be stored at -20°C upon arrival.

All kit components should remain stable for at least 1 year when stored at the specified storage conditions.

StepComponentCat. 70100 (24 preps)Cat. 70110, 70120, 70130, 70140 (96 preps)
Amplicon PCR (PCR 1)MGX Master Mix330 µL1,320 µL
16S V1-V2 Primer Mix70 µL280 µL
Index PCR (PCR 2)Indexing Master Mix660 µL2 x 1,320 µL
N7xx Index Primer50 µL50 µL
S5xx Index Primer70 µL70 µL
PCR Clean-UpResuspension Buffer2 x 1,250 µL2 x 5,000 µL
Nuclease-free water1,250 µL1 x 6,000 µL

Tetra(3-methoxy-N-(PEG5-prop-2-ynyl)propanamide) Methane

Tetra(3-methoxy-N-(PEG5-prop-2-ynyl)propanamide) Methane is a branched crosslinker with four terminal propargyl groups. The propargyl groups can react with azide compounds or biomolecules via copper catalyzed Click Chemistry to form a stable triazole linkage.