Lactose/Sucrose/D-Glucose Assay Kit

Everything you need to run a trial PACE Genotyping Reaction on your existing lab equipment. Each PACE Trial Kit includes Test DNA samples, PACE Genotyping Assays, PACE Master Mix and a comprehensive PACE Genotyping Trial Kit Manual.

About

Everything you need to run a trial PACE® allele-specific PCR Genotyping Reaction on your existing lab equipment. Each PACE Trial Kit includes Test DNA samples, PACE Genotyping Assays, PACE Master Mix and a comprehensive PACE Genotyping Trial Kit Manual.

WHO IS THIS TRIAL KIT FOR?

Anyone who wants to try PACE genotyping reagents in their lab for the first time with a set of validated DNA samples, SNP assays and PACE Master Mix.

TRIAL KIT OVERVIEW

Step 1. Dispense each of the three trial DNA samples (DNA 1, 2 and 3) plus water (No Template Control) in triplicate onto a PCR plate using the suggested volumes.

Step 2. Combine appropriate volumes of PACE Genotyping Master Mix with PACE Genotyping Assay in a tube, as directed, then mix.

Step 3. Dispense the combined mixtures into each of the wells containing DNA using volumes indicated. Each test now contains a complete PACE Genotyping Reaction.

Step 4. Seal your PCR plate with an optically clear seal and centrifuge to ensure all components are at the bottom of the wells.

Step 5.Thermally cycle the reaction plate using the thermal cycling conditions provided.

Step 6. Read the plate and compare data produced with the expected results provided in the manual. Simple!

PACE MECHANISM

More information on the PACE genotyping chemistry and how it works can be found here: www.3crbio.com/#pace. PACE allele-specific PCR is used for the detection of SNPs, Indels and other sequence variants.

REQUIRED COMPONENTS

• qPCR machine or Thermocycler + Fluorescent plate reader
• PCR plate or equivalent and appropriate optically clear seal
• PCR-grade water

qPCR machine or Thermocycler + Fluorescent plate reader

PCR plate or equivalent and appropriate optically clear seal

PCR-grade water

DBCO-PEG12-acid is an analog of DBCO-Acid with hydrophilic PEG linker and a DBCO group. The DBCO groups is commonly used for Click Chemistry reactions. The hydrophilic PEG chain allows for increased water solubility of compounds in aqueous media. The terminal carboxylic acid can react with primary amine groups in the presence of activators (e.g. EDC, or HATU) to form a stable amide bond. Reagent grade, for research purpose. Please contact us for GMP-grade inquiries.

DBCO-PEG12-acid is an analog of DBCO-Acid with hydrophilic PEG linker and a DBCO group. The DBCO groups is commonly used for Click Chemistry reactions. The hydrophilic PEG chain allows for increased water solubility of compounds in aqueous media. The terminal carboxylic acid can react with primary amine groups in the presence of activators (e.g. EDC, or HATU) to form a stable amide bond. Reagent grade, for research purpose. Please contact us for GMP-grade inquiries.

DBCO-PEG8-PFP ester is a PEG reagent comprising a DBCO group which can react with azides for copper-free Click Chemistry reactions. The PFP ester is an active ester which can be used to react with amine groups. PFP esters have been are stable compounds and are less susceptible to undergo hydrolysis. PEG8 linker increases the water solubility of a compound in aqueous media. Reagent grade, for research purpose. Please contact us for GMP-grade inquiries.

DBCO-PEG8-PFP ester is a PEG reagent comprising a DBCO group which can react with azides for copper-free Click Chemistry reactions. The PFP ester is an active ester which can be used to react with amine groups. PFP esters have been are stable compounds and are less susceptible to undergo hydrolysis. PEG8 linker increases the water solubility of a compound in aqueous media. Reagent grade, for research purpose. Please contact us for GMP-grade inquiries.