Description

Nucleic acid testing (NAT) is the method of choice for detection and quantification of a wide range of micro organisms. Primerdesign manufactures and supplies high quality quantitative real-time PCR kits for the detection and simultaneous quantification of numerous significant pathogens . A copy number standard curve is provided for quantification and an the internal extraction template (DNA or RNA), controls for the quality of the nucleic acid extraction and eliminates false negative results.

The kit is designed with the broadest possible detection profile to ensure that all clinically relevant strains and subtypes are detected. Target sequences are selected by working with data from key opinion leaders in the field. Multiple sequence alignments and unprecedented real-time PCR expertise in design and validation ensure the best possible kit. Details of the target and priming specificity are included in the individual handbooks above.

Packaged, optimised and ready to use. Expect Better Data.

Primer and probe mix (150 reactions)
Reverse Transcription, target specific primers (RNA genome viruses only)
Copy number standard curve (sufficient for multiple standard curves)
Internal extraction control – Read through VIC channel*
Endogenous control (150 tests)
RNAse/DNAse free water
*alternative fluorophores available on request

Introduction

This kit provides a simple and fast solution for the extraction of circulating nuclear acid from serum, plasma, and other cell-free liquid samples. Circulating nucleic acid refers to the free extracellular nucleic acid produced by cell apoptosis, of which fragments are generally below 1KB. The kit is based on silica gel column purification technology, which is no need for toxic phenol chloroform extraction and time-consuming alcohol precipitation during the extraction. The obtained Circulating Nucleic Acid can be directly used for quantitative PCR, liquid or solid phase chip analysis, hybridization, and SNP detection.
HiPure Circulating DNA/RNA Kit adopts a unique solution system and multiple layers of filter membranes with different pore sizes, which can efficiently process large volumes of serum and plasma samples and capture extremely small amounts of free nucleic acids.

Details

Specifications

Features	Specifications
Main Functions	Isolation both Circulating DNA/RNA (include miRNA) from 1-5 ml serum and plasma
Applications	qPCR / RT-PCR, liquid or solid-phasechip analysis, hybridization and SNP detection
Purification method	Midi spin column
Purification technology	Silica technology, DNA filtration technology
Process method	Manual (centrifugation or vacuum)
Sample type	serum, plasma, and other cell-free liquid samples
Sample amount	1-5 ml
Elution volume	≥20μl
Time per run	≤100 minutes
Liquid carrying volume per column	4 ml
Binding yield of column	1 mg

Principle

This kit is based on silica gel column technology. Serum or other liquid samples are lysed and digested in buffer CFL. After adding buffer CFP, the protein is removed by centrifugation to obtain the supernatant. Isopropanol is added to precipitate the total nucleic acid and transferred to the column for filtration. DNA / RNA is adsorbed on the membrane of the column, while the protein is not adsorbed and removed with the filtrate.The column is washed with buffer MGW1 to remove protein and other impurities, and then washed with buffer RW2 to remove salt. Finally, DNA / RNA is eluted by low salt buffer. The eluted DNA / RNA can be directly used for quantitative PCR/ RT-PCR, liquid or solid-phase chip analysis, hybridization and SNP detection.

Advantages

• High yield – most optimized process to obtain maximum free RNA and small RNA
• High concentration – low elution volume (>20μl) to ensure nucleic acid concentration
• High purity – low alcohol combination, completely remove inhibitor and protein pollution
• High recovery – silica gel column technology can recover nucleic acid molecules at the level of PG
• Large volume – 1-2ml serum and plasma samples can be processed at one time

Kit Contents

Contents	R431602	D431603
Purification Times	50 Preps	250 Preps
HiPure RNA Micro Columns	50	5 x 50
HiPure Viral Midi Columns	50	5 x 50
15 ml Collection Tubes	50	5 x 50
2ml Collection Tubes	50	5 x 50
Buffer CFL	150 ml	2 x 375 ml
Buffer CFP	30 ml	150 ml
Buffer MGW1*	100 ml	2 x 250 ml
Buffer RW2*	2 x 50 ml	5 x 100 ml
RNase Free Water	10 ml	50 ml

Storage and Stability

The kit components can be stored at room temperature (15–25°C) and are stable for at least 18 months under these conditions.

This kit provides a simple and fast solution for the extraction of circulating nuclear acid from serum, plasma, and other cell-free liquid samples. Circulating nucleic acid refers to the free extracellular nucleic acid produced by cell apoptosis, of which fragments are generally below 1KB. The kit is based on silica gel column purification technology, which is no need for toxic phenol chloroform extraction and time-consuming alcohol precipitation during the extraction. The obtained Circulating Nucleic Acid can be directly used for quantitative PCR, liquid or solid phase chip analysis, hybridization, and SNP detection.
HiPure Circulating DNA/RNA Kit adopts a unique solution system and multiple layers of filter membranes with different pore sizes, which can efficiently process large volumes of serum and plasma samples and capture extremely small amounts of free nucleic acids.

Introduction

This product is suitable for rapid extraction of DNA from FFPE sample. This kit uses two combination methods. High-salt Bind is conducive to remove pigments or polysaccharides from complex FFPE samples, so as to improve the purity of nucleic acid and avoid blocking aligent 2100. Alcohol mediated adsorption is conducive to improve the nucleic acid yield of high-yield samples.

Details

Specifications

Features	Specifications
Main Functions	Isolation high pure total DNA from FFPE using high bind beads
Applications	PCR and viral DNA detection, etc.
Purification technology	Magnetic beads technology
Process method	Manual or automatic
Sample type	Paraffin embedded tissue samples
Sample amount	1-6 slices of 10-20μm
Elution volume	≥30μl
Time per run	≤60 minutes

Principle

This product is based on the purification method of high binding magnetic particles. The sample is lysed and digested under the action of lysate and Protease. DNA is released into the lysate. After adding magnetic particles and binding solution, DNA will be adsorbed on the surface of magnetic particles, and impurities such as proteins will be removed without adsorption. The adsorbed particles were washed with washing solution to remove proteins and impurities, washed with ethanol to remove salts, and finally DNA was eluted by Elution Buffer.

Advantages

• High yield – most optimal process, recovery up to 90%
• Economy – less than 50% of the price of Qiagen and other imported products
• High purity – OD 260/280 : 1.7-1.9, OD 260/230 : 1.5-2.0

Kit Contents

Contents	D632301D	D632302D
Purification Times	48 Preps	96 Preps
MagPure Particles N	1.1 ml	2.5 ml
RNase A	10 mg	20 mg
Proteinase K	24 mg	48 mg
Protease Dissolve Buffer	3 ml	6 ml
Buffer DPS	60 ml	100 ml
Buffer ATL	15 ml	30 ml
Buffer BST1	30 ml	60 ml
Buffer BW1	13 ml	44 ml
Elution Buffer	15 ml	30 ml

Storage and Stability

Proteinase K, RNase A and MagPure Particles N should be stored at 2-8°C upon arrival. However, short-term storage (up to 12 weeks) at room temperature (15-25°C) does not affect their performance. The remaining kit components can be stored at room temperature (15-25°C) and are stable for at least 18 months under these conditions.

This product is suitable for rapid extraction of DNA from FFPE sample. This kit uses two combination methods. High-salt Bind is conducive to remove pigments or polysaccharides from complex FFPE samples, so as to improve the purity of nucleic acid and avoid blocking aligent 2100. Alcohol mediated adsorption is conducive to improve the nucleic acid yield of high-yield samples.